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A novel photoactive GAF domain of cyanobacteriochrome AnPixJ that shows reversible green/red photoconversion.

Abstract：

:We report the discovery of a novel cyanobacteriochrome, the green/red photoreceptor AnPixJ (All1069), isolated from the heterocyst-forming cyanobacterium Anabaena (Nostoc) sp. PCC 7120. Cyanobacteriochromes are a recently emerging tetrapyrrole-based photoreceptor superfamily that are distantly related to the conventional red/far-red photoreceptor phytochromes (Phys). The chromophore-binding domains of AnPixJ produced in cyanobacterial and Escherichia coli cells both showed a reversible and full photoconversion between a green-absorbing form (lambda(max)=543 nm) and a red-absorbing form (lambda(max)=648 nm). Denaturation analysis revealed that the green-absorbing form and the red-absorbing form covalently ligated phycocyanobilin with E-configuration and Z-configuration at the C15C16 double bond, respectively. Time-resolved spectral analysis showed the formation of the first intermediate state peaking at 680 nm from the dark-stable red-absorbing form. This step resembles the first photoconversion step from the red-absorbing form to the red-shifted lumi-R intermediate state of the Phys. These results suggest that the Pr of AnPixJ is almost equivalent to that of the Phys and starts a primary photoreaction with Z-to-E isomerization in a mechanism similar to that in the Phys, but is finally photoconverted to the unique green-absorbing form.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Narikawa R,Fukushima Y,Ishizuka T,Itoh S,Ikeuchi M

doi

10.1016/j.jmb.2008.05.035

subject

Has Abstract

pub_date

2008-07-25 00:00:00

pages

844-55

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(08)00601-3

journal_volume

380

pub_type

杂志文章

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