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Increased production of a knotted form of plasmid pBR322 DNA in Escherichia coli DNA topoisomerase mutants.

Abstract：

:Plasmid pBR322 prepared from Escherichia coli strains carrying deletion of the DNA topoisomerase I gene (delta topA) with a compensatory mutation of the DNA gyrase gene (gyrA or gyrB) and from their TopA+ transductants was analyzed by agarose gel electrophoresis followed by electron microscopy, and compared with that from isogenic wild-type strains. It was found that about 1% of the plasmid DNA molecules was a knotted species in the topA+ gyr+ strains W3110 and DM4100, while strains DM750 (delta topA gyrA224), DM800 (delta topA gyrB225), SD275 (topA+ gyrA224) and SD108 (topA+ gyrB225) produced six to ten times as much knotted DNA as the topA+ gyr+ controls. The results suggest that the increased production of knotted pBR322 DNA is closely related to mutations of the gyrase genes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Shishido K,Komiyama N,Ikawa S

doi

10.1016/0022-2836(87)90338-x

subject

Has Abstract

pub_date

1987-05-05 00:00:00

pages

215-8

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90338-X

journal_volume

195

pub_type

杂志文章

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