The preprotein translocase of the inner mitochondrial membrane: evolutionary conservation of targeting and assembly of Tim17.

abstract：:The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA compl...

journal_title：Journal of molecular biology

authors： Gerdes K,Helin K,Christensen OW,Løbner-Olesen A

更新日期：1988-09-05 00:00:00

abstract：:Stochastic fluctuations, termed "noise," in the level of biological molecules can greatly impact cellular functions. While biological noise can sometimes be detrimental, recent studies have provided an increasing number of examples in which biological noise can be functionally beneficial. Rather than provide an exhaus...

journal_title：Journal of molecular biology

authors： Harton MD,Batchelor E

更新日期：2017-04-21 00:00:00

abstract：:The expression of xylose utilization in Bacillus subtilis is regulated at the level of transcription by xylose dependent Xyl repressor-xyl operator interaction. We have structurally and functionally characterized the binding sites of Xyl repressor in the xyl regulatory region. Methylation and hydroxyl radical protecti...

journal_title：Journal of molecular biology

authors： Dahl MK,Degenkolb J,Hillen W

更新日期：1994-10-28 00:00:00

abstract：:RNA helicases play fundamental roles in modulating RNA structures and facilitating RNA-protein (RNP) complex assembly in vivo. Previously, our laboratory demonstrated that the DEAD-box RNA helicase Dbp2 in Saccharomyces cerevisiae is required to promote efficient assembly of the co-transcriptionally associated mRNA-bi...

journal_title：Journal of molecular biology

authors： Ma WK,Paudel BP,Xing Z,Sabath IG,Rueda D,Tran EJ

更新日期：2016-03-27 00:00:00

abstract：:The Fv fragment from an anti-dansyl antibody was optimally crystallized into two crystal forms having slightly different lattice dimensions at pH 5.25 and 6.75. The two crystal structures were determined and refined at high resolution at 112 K (at 1.45 A for the crystal at pH 5.25 and at 1.55 A for that at pH 6.75). I...

journal_title：Journal of molecular biology

authors： Nakasako M,Takahashi H,Shimba N,Shimada I,Arata Y

更新日期：1999-08-06 00:00:00

abstract：:During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic pathways: deformylation of the N-terminal methionine by the enzyme peptide deformylase (PDF), followed by methionine excision ...

journal_title：Journal of molecular biology

authors： Bhakta S,Akbar S,Sengupta J

更新日期：2019-03-29 00:00:00

abstract：:The X-ray crystal structure of a complex of a modified recombinant kringle-2 domain of human plasminogen, K2Pg[C4G/E56D/L72Y] (mK2Pg), containing an upregulated lysine-binding site, bound to a functional 30 residue internal peptide (VEK-30) from an M-type protein of a group A Streptococcus surface protein, has been de...

journal_title：Journal of molecular biology

authors： Rios-Steiner JL,Schenone M,Mochalkin I,Tulinsky A,Castellino FJ

更新日期：2001-05-11 00:00:00

abstract：:Group II intron retrohoming occurs by a mechanism in which the intron RNA reverse splices directly into one strand of a double-stranded DNA target site, while the intron-encoded reverse transcriptase uses a C-terminal DNA endonuclease activity to cleave the opposite strand and then uses the cleaved 3' end as a primer ...

journal_title：Journal of molecular biology

authors： San Filippo J,Lambowitz AM

更新日期：2002-12-13 00:00:00

abstract：:Anthracycline antibiotics daunomycin and adriamycin are among the most widely used in cancer chemotherapy and DNA is believed to be the primary target of their biological action. The crystal structure of a morpholino derivative of adriamycin bound to the DNA hexamer d(CGTACG) has been determined at 1.5 A resolution. T...

journal_title：Journal of molecular biology

authors： Cirilli M,Bachechi F,Ughetto G,Colonna FP,Capobianco ML

更新日期：1993-04-05 00:00:00

abstract：:We describe a selection procedure for construction of very high affinity variants of human growth hormone (hGH) for binding to the extra cellular domain of its receptor (called the hGHbp). Five different libraries of mutated hGH genes (each containing approximately 2 x 10(5) protein variants) were created by randomly ...

journal_title：Journal of molecular biology

authors： Lowman HB,Wells JA

更新日期：1993-12-05 00:00:00

abstract：:The active site of influenza virus neuraminidase (NA) is formed by 11 universally conserved residues. A guanidino group incorporated into two unrelated NA inhibitors was previously reported to occupy different negatively charged sites in the NA active site, A new inhibitor containing two guanidino groups was synthesiz...

journal_title：Journal of molecular biology

authors： Sudbeck EA,Jedrzejas MJ,Singh S,Brouillette WJ,Air GM,Laver WG,Babu YS,Bantia S,Chand P,Chu N,Montgomery JA,Walsh DA,Luo M

更新日期：1997-04-04 00:00:00

abstract：:TWINKLE is the helicase at the mitochondrial DNA (mtDNA) replication fork in mammalian cells. Mutations in the PEO1 gene, which encodes TWINKLE, cause autosomal dominant progressive external ophthalmoplegia (AdPEO), a disorder associated with deletions in mtDNA. Here, we characterized seven different AdPEO-causing mut...

journal_title：Journal of molecular biology

authors： Korhonen JA,Pande V,Holmlund T,Farge G,Pham XH,Nilsson L,Falkenberg M

更新日期：2008-03-28 00:00:00

abstract：:The decrease in conformational stability, delta(delta G), has been measured for 72 aliphatic side-chain mutants from four proteins in which a larger side-chain is replaced by a smaller side-chain so that steric effects are minimal. When these delta(delta G) values are corrected to the same accessibility, namely 100% b...

journal_title：Journal of molecular biology

authors： Pace CN

更新日期：1992-07-05 00:00:00

abstract：:We have used nuclear magnetic resonance (NMR) to obtain the structure of an RNA "kissing" hairpin complex formed between the HIV-2 TAR hairpin loop and a hairpin with a complementary loop sequence. Kissing hairpins are important in natural antisense reactions; their complex is a specific target for protein binding. Th...

journal_title：Journal of molecular biology

authors： Chang KY,Tinoco I Jr

更新日期：1997-05-30 00:00:00

abstract：:Many medically relevant Gram-negative bacteria use the type III secretion system (T3SS) to translocate effector proteins into the host for their invasion and intracellular survival. A multi-protein complex located at the cytosolic interface of the T3SS is proposed to act as a sorting platform by selecting and targetin...

journal_title：Journal of molecular biology

authors： Bernal I,Börnicke J,Heidemann J,Svergun D,Horstmann JA,Erhardt M,Tuukkanen A,Uetrecht C,Kolbe M

更新日期：2019-09-06 00:00:00

abstract：:The leghaemoglobins have oxygen affinities 11 to 24 times higher than that of sperm whale myoglobin, due mainly to higher rates of association. To find out why, we have determined the structures of deoxy- and oxy-leghaemoglobin II of the lupin at 1.7 A resolution. Results confirm the general features found in previous...

journal_title：Journal of molecular biology

authors： Harutyunyan EH,Safonova TN,Kuranova IP,Popov AN,Teplyakov AV,Obmolova GV,Rusakov AA,Vainshtein BK,Dodson GG,Wilson JC

更新日期：1995-08-04 00:00:00

abstract：:Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first ...

journal_title：Journal of molecular biology

authors： Tian F,Cross TA

更新日期：1999-02-05 00:00:00

abstract：:Tumor suppressor p53 plays a central role in tumor suppression. p53 is the most frequently mutated gene in human cancer, and over half of human cancers contain p53 mutations. Majority of p53 mutations in cancer are missense mutations, leading to the expression of full-length mutant p53 (mutp53) protein. While the crit...

journal_title：Journal of molecular biology

authors： Yue X,Zhao Y,Xu Y,Zheng M,Feng Z,Hu W

更新日期：2017-06-02 00:00:00

abstract：:Meiotic clade AAA ATPases (ATPases associated with diverse cellular activities), which were initially grouped on the basis of phylogenetic classification of their AAA ATPase cassette, include four relatively well characterized family members, Vps4, spastin, katanin and fidgetin. These enzymes all function to disassemb...

journal_title：Journal of molecular biology

authors： Monroe N,Hill CP

更新日期：2016-05-08 00:00:00

abstract：:The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints ...

journal_title：Journal of molecular biology

authors： Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

更新日期：1988-09-05 00:00:00

abstract：:Here, we show that in the chicken genome, the domain of alpha-globin genes is preceded by a CpG island of which the downstream part ( approximately 0.65 kbp) is heavily methylated in lymphoid cells; it is either non-methylated or undermethylated in erythroid cells. Recombinant plasmids were constructed with the corres...

journal_title：Journal of molecular biology

authors： Razin SV,Ioudinkova ES,Scherrer K

更新日期：2000-06-16 00:00:00

abstract：:The MA (matrix) domain of the retroviral Gag polyprotein plays several critical roles during virus assembly. Although best known for targeting the Gag polyprotein to the inner leaflet of the plasma membrane for virus budding, recent studies have revealed that MA also contributes to selective packaging of the genomic R...

journal_title：Journal of molecular biology

authors： Parent LJ,Gudleski N

更新日期：2011-07-22 00:00:00

abstract：:Mutational experiments show how changes in the hydrophobic cores of proteins affect their stabilities. Here, we estimate these effects computationally, using four-body likelihood potentials obtained by simplicial neighborhood analysis of protein packing (SNAPP). In this procedure, the volume of a known protein structu...

journal_title：Journal of molecular biology

authors： Carter CW Jr,LeFebvre BC,Cammer SA,Tropsha A,Edgell MH

更新日期：2001-08-24 00:00:00

abstract：:IL-22 is a class 2 alpha-helical cytokine involved in the generation of inflammatory responses. These activities require IL-22 to engage the cell surface receptors IL-22R1 and the low-affinity signaling molecule IL-10R2. IL-10R2 also interacts with five other class 2 cytokines: IL-10, IL-26, and the interferon-like cy...

journal_title：Journal of molecular biology

authors： Logsdon NJ,Jones BC,Allman JC,Izotova L,Schwartz B,Pestka S,Walter MR

更新日期：2004-09-10 00:00:00

abstract：:The backbone dynamics of the cytokine interleukin-8, a symmetric homodimer of overall molecular mass 16 kDa, has been investigated at pH 5.2 by means of 15N relaxation measurements using heteronuclear two-dimensional inverse detected 1H-15N spectroscopy. 15N T1, T2 and NOE data were obtained for 66 out of a total of 6...

journal_title：Journal of molecular biology

authors： Grasberger BL,Gronenborn AM,Clore GM

更新日期：1993-03-20 00:00:00

abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

journal_title：Journal of molecular biology

authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

更新日期：1993-10-20 00:00:00

abstract：:Single sodium-driven rotors from a bacterial ATP synthase were embedded into a lipid membrane and observed in buffer solution at subnanometer resolution using atomic force microscopy (AFM). Time-lapse AFM topographs show the movement of single proteins within the membrane. Subsequent analysis of their individual traje...

journal_title：Journal of molecular biology

authors： Müller DJ,Engel A,Matthey U,Meier T,Dimroth P,Suda K

更新日期：2003-04-11 00:00:00

abstract：:Human islet amyloid polypeptide (hIAPP), which is considered the primary culprit for beta-cell loss in type 2 diabetes mellitus patients, is synthesized in beta-cells of the pancreas from its precursor pro-islet amyloid polypeptide (proIAPP), which may be important in early intracellular amyloid formation as well. We ...

journal_title：Journal of molecular biology

authors： Jha S,Sellin D,Seidel R,Winter R

更新日期：2009-06-26 00:00:00

abstract：:Interaction of the leader RNA with the unphosphorylated P-protein has been proposed to play a key role in the transcription-replication transition of Chandipura virus, a model rhabdovirus. Electrophoretic mobility shift assay with the leader RNA and the unphosphorylated P-protein demonstrated existence of two distinct...

journal_title：Journal of molecular biology

authors： Basak S,Polley S,Basu M,Chattopadhyay D,Roy S

更新日期：2004-06-18 00:00:00

abstract：:Integration host factor (IHF) of Escherichia coli is a histone-like protein that is involved both in site-specific recombination and in regulating the expression of a number of phage and bacterial genes. We have shown previously that transcription of the ilvGMEDA operon in E. coli is greatly reduced in IHF mutants. We...

journal_title：Journal of molecular biology

authors： Tsui P,Freundlich M

更新日期：1988-10-05 00:00:00