Preliminary crystallographic analysis of bovine enterovirus.

abstract：:NHP6A is a non-sequence-specific DNA-binding protein from Saccharomyces cerevisiae which belongs to the HMGB protein family. Previously, we have solved the structure of NHP6A in the absence of DNA and modeled its interaction with DNA. Here, we present the refined solution structures of the NHP6A-DNA complex as well as...

journal_title：Journal of molecular biology

authors： Masse JE,Wong B,Yen YM,Allain FH,Johnson RC,Feigon J

更新日期：2002-10-18 00:00:00

abstract：:We present a refined model of the membrane-associated Torpedo acetylcholine (ACh) receptor at 4A resolution. An improved experimental density map was obtained from 342 electron images of helical tubes, and the refined structure was derived to an R-factor of 36.7% (R(free) 37.9%) by standard crystallographic methods, a...

journal_title：Journal of molecular biology

authors： Unwin N

更新日期：2005-03-04 00:00:00

abstract：:The molecular mechanisms underlying transcription elongation and their role in gene regulation are poorly characterized in eukaryotes. A number of genes, however, have been proposed to be regulated at the level of transcription elongation, including c-myc, c-fos and c-myb. Here, we analyze the control of transcription...

journal_title：Journal of molecular biology

authors： Pinaud S,Mirkovitch J

更新日期：1998-07-31 00:00:00

abstract：:Protein tyrosine phosphatase PTPN13, also known as PTP-BL in mice, represents a large multi-domain non-transmembrane scaffolding protein that contains five consecutive PDZ domains. Here, we report the solution structures of the extended murine PTPN13 PDZ3 domain in its apo form and in complex with its physiological li...

journal_title：Journal of molecular biology

authors： Kock G,Dicks M,Yip KT,Kohl B,Pütz S,Heumann R,Erdmann KS,Stoll R

更新日期：2018-10-19 00:00:00

abstract：:DDX3X is a conserved DEAD-box RNA helicase involved in translation initiation and other processes of RNA metabolism. Mutations in human DDX3X and deregulation of its expression are linked to tumorigenesis and intellectual disability. The protein is also targeted by diverse viruses. Previous studies demonstrated helica...

journal_title：Journal of molecular biology

authors： Sharma D,Putnam AA,Jankowsky E

更新日期：2017-11-24 00:00:00

abstract：:Structures of glycoconjugate N-glycans and glycolipids of invertebrates show significant differences from those of vertebrates. These differences are due largely to the vertebrate beta1,4-galactosyltransferase-1 (beta4Gal-T1), which is found as a beta1,4-N-acetylgalactosaminyltransferase (beta4GalNAc-T1) in invertebra...

journal_title：Journal of molecular biology

authors： Ramakrishnan B,Qasba PK

更新日期：2007-01-19 00:00:00

abstract：:Three viral proteins participate directly in transcription of bacteriophage T4 late genes: the sigma-family protein gp55 provides promoter recognition, gp33 is the co-activator, and gp45 is the activator of transcription; gp33 also represses transcription in the absence of gp45. Transcriptional activation by gp45, the...

journal_title：Journal of molecular biology

authors： Kolesky SE,Ouhammouch M,Geiduschek EP

更新日期：2002-08-30 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:The Rev-erb family is an orphan nuclear receptor acting as a negative regulator of transcription. Rev-erbalpha and Rev-erbbeta are crucial components of the circadian clock and involved in various lipid homeostasis. They are unique nuclear receptors that lack the activation function 2 helix (AF2-helix) required for li...

journal_title：Journal of molecular biology

authors： Woo EJ,Jeong DG,Lim MY,Jun Kim S,Kim KJ,Yoon SM,Park BC,Ryu SE

更新日期：2007-10-26 00:00:00

abstract：:The viral RNA-dependent RNA polymerases show a conserved structure where the fingers domain interacts with the top of the thumb domain to create a tunnel through which nucleotide triphosphates reach the active site. We have solved the crystal structures of poliovirus polymerase (3D(pol)) in complex with all four NTPs,...

journal_title：Journal of molecular biology

authors： Thompson AA,Albertini RA,Peersen OB

更新日期：2007-03-09 00:00:00

abstract：:The heterogeneous nuclear ribonucleoprotein K protein is an RNA-binding protein found in several subcellular compartments where it is thought to be involved in signaling multiple processes that compose gene expression. K protein contains three K homology (KH) domains that mediate RNA-binding. We used a serial analysis...

journal_title：Journal of molecular biology

authors： Klimek-Tomczak K,Wyrwicz LS,Jain S,Bomsztyk K,Ostrowski J

更新日期：2004-09-24 00:00:00

abstract：:Here, we report a new computational method, called sheetminer, for mining beta-sheets in the density maps at intermediate resolutions of 6 to 10A. The method employs a multi-step ad hoc morphological analysis of density maps to identify the unique characteristics of beta-sheets. It was tested on density maps from 12 p...

journal_title：Journal of molecular biology

authors： Kong Y,Ma J

更新日期：2003-09-12 00:00:00

abstract：:A repetitive DNA sequence family from Xenopus laevis that has an unusual genomic organization has been identified. It has been shown by blot-hybridization that this sequence occurs in clusters containing variable numbers of the 388 base-pair repeating unit. There are approximately 500 such clusters in the genome, and ...

journal_title：Journal of molecular biology

authors： Lam BS,Carroll D

更新日期：1983-04-25 00:00:00

abstract：:Reverse transcriptases (RTs) encoded by a wide range of mobile retroelements have had a major impact on the structure and function of genomes. Among the most abundant elements in eukaryotes are the non long terminal repeat (LTR) retrotransposons. Here we compare the dNTP concentration requirements and error rates of t...

journal_title：Journal of molecular biology

authors： Jamburuthugoda VK,Eickbush TH

更新日期：2011-04-15 00:00:00

abstract：:Signaling photoreceptors mediate diverse organismal adaptations in response to light. As light-gated protein switches, signaling photoreceptors provide the basis for optogenetics, a term that refers to the control of organismal physiology and behavior by light. We establish as novel optogenetic tools the plasmids pDus...

journal_title：Journal of molecular biology

authors： Ohlendorf R,Vidavski RR,Eldar A,Moffat K,Möglich A

更新日期：2012-03-02 00:00:00

abstract：:gp41 is the protein responsible for the process of membrane fusion that allows primate lentiviruses (HIV and SIV) to enter into their host cells. gp41 ectodomain contains an N-terminal and a C-terminal heptad repeat region (NHR and CHR) connected by an immunodominant loop. In the absence of membranes, the NHR and CHR ...

journal_title：Journal of molecular biology

authors： Peisajovich SG,Blank L,Epand RF,Epand RM,Shai Y

更新日期：2003-03-07 00:00:00

abstract：:The regulation of disulphide stress in actinomycetes such as Streptomyces coelicolor is known to involve the zinc-containing anti-sigma factor RsrA that binds and inactivates the redox-regulated sigma factor sigmaR. However, it is not known how RsrA senses disulphide stress nor what role the metal ion plays. Using in ...

journal_title：Journal of molecular biology

authors： Li W,Bottrill AR,Bibb MJ,Buttner MJ,Paget MS,Kleanthous C

更新日期：2003-10-17 00:00:00

abstract：:It is generally believed that DNA replication in most eukaryotes proceeds according to a precise program in which there is a defined temporal order by which each chromosomal region is duplicated. However, the regularity of this program at the level of individual chromosomes, in terms of both the relative timing and th...

journal_title：Journal of molecular biology

authors： Czajkowsky DM,Liu J,Hamlin JL,Shao Z

更新日期：2008-01-04 00:00:00

abstract：:We have collected Laue diffraction data from crystals of tomato bushy stunt virus using the full white X-ray spectrum from the wiggler magnet of the Synchrotron Radiation Source at Daresbury, U.K. A single 24 second exposure of a crystal soaked in EDTA yielded a data set that was 90% complete between 6 and 3.5 A resol...

journal_title：Journal of molecular biology

authors： Campbell JW,Clifton IJ,Greenhough TJ,Hajdu J,Harrison SC,Liddington RC,Shrive AK

更新日期：1990-08-05 00:00:00

abstract：:Interactions within the decoding center of the 30 S ribosomal subunit have been investigated by constructing all 15 possible mutations at nucleotides C1402 and A1500 in helix 44 of 16 S rRNA. As expected, most of the mutations resulted in highly deleterious phenotypes, consistent with the high degree of conservation o...

journal_title：Journal of molecular biology

authors： Vila-Sanjurjo A,Dahlberg AE

更新日期：2001-05-04 00:00:00

abstract：:The cobalamin-independent methionine synthase enzyme catalyzes a challenging reaction: the direct transfer of a methyl from 5-methyl-tetrahydrofolate-glutamate3 to the l-homocysteine thiol. The enzyme has a dual (βα)8 TIM barrel structure that binds, activates and brings the reactants into reaction proximity by confor...

journal_title：Journal of molecular biology

authors： Ubhi DK,Robertus JD

更新日期：2015-02-27 00:00:00

abstract：:The folded 10 S monomer conformation of smooth muscle myosin traps the hydrolysis products ADP and Pi in its active sites. To test the significance of this, we have searched for equivalent trapping in other conformational and assembly states of avian gizzard and brush border myosins, using formycin triphosphate (FTP) ...

journal_title：Journal of molecular biology

authors： Cross RA,Jackson AP,Citi S,Kendrick-Jones J,Bagshaw CR

更新日期：1988-09-05 00:00:00

abstract：:During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic pathways: deformylation of the N-terminal methionine by the enzyme peptide deformylase (PDF), followed by methionine excision ...

journal_title：Journal of molecular biology

authors： Bhakta S,Akbar S,Sengupta J

更新日期：2019-03-29 00:00:00

abstract：:Ras proteins regulate a wide range of biological processes by interacting with a variety of effector proteins. In addition to the known role in tumorigensis, the activated form of Ras exhibits growth-inhibitory effects by unknown mechanisms. Several Ras effector proteins identified as mediators of apoptosis and cell-c...

journal_title：Journal of molecular biology

authors： Kumari G,Singhal PK,Rao MR,Mahalingam S

更新日期：2007-04-13 00:00:00

abstract：:Natural cellulose exists as a composite of different forms, which have historically been broadly characterized as "crystalline" or "amorphous". The recognition of both of these forms of cellulose by the carbohydrate-binding modules (CBM) of microbial glycoside hydrolases is central to natural and efficient biotechnolo...

journal_title：Journal of molecular biology

authors： Jamal S,Nurizzo D,Boraston AB,Davies GJ

更新日期：2004-05-28 00:00:00

abstract：:During the late phase of human immunodeficiency virus type-1 (HIV-1) replication, newly synthesized retroviral Gag proteins are targeted to lipid raft regions of specific cellular membranes, where they assemble and bud to form new virus particles. Gag binds preferentially to the plasma membrane (PM) of most hematopoie...

journal_title：Journal of molecular biology

authors： Saad JS,Loeliger E,Luncsford P,Liriano M,Tai J,Kim A,Miller J,Joshi A,Freed EO,Summers MF

更新日期：2007-02-16 00:00:00

abstract：:DNase I digestion of unlabelled chromatin has been used to determine the orientation of nucleosomes in the higher-order structure of chromatin. DNA digestion patterns were analysed quantitatively and compared with simulation curves that were generated with the experimentally obtained rate constants for cutting inside ...

journal_title：Journal of molecular biology

authors： Staynov DZ,Proykova YG

更新日期：1998-05-29 00:00:00

abstract：:The crystal structure of human sex hormone-binding globulin (SHBG) has revealed how 5alpha-dihydrotestosterone intercalates between the two seven-stranded beta-sheets of its amino-terminal laminin G-like domain. However, a region of disorder (residues 130 to 135 of SHBG) was identified together with a zinc-binding sit...

journal_title：Journal of molecular biology

authors： Grishkovskaya I,Avvakumov GV,Hammond GL,Muller YA

更新日期：2002-05-03 00:00:00

abstract：:Spherulin 3a (S3a) from Physarum polycephalum represents the only known single-domain member of the superfamily of beta gamma eye-lens crystallins. It shares the typical two Greek-key motif and is stabilized by dimerization and Ca(2+)-binding. The temperature and denaturant-induced unfolding of S3a in the absence and ...

journal_title：Journal of molecular biology

authors： Kretschmar M,Jaenicke R

更新日期：1999-09-03 00:00:00

abstract：:We have determined the X-ray crystallographic structure of trout Hb I in both the deoxy and carbonmonoxy forms to resolution limits of 2.3 angstroms and 2.5 angstroms, respectively. The overall fold of the molecule is highly similar to that of human HbA despite the low level of sequence identity between these proteins...

journal_title：Journal of molecular biology

authors： Tame JR,Wilson JC,Weber RE

更新日期：1996-06-21 00:00:00