The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach.

abstract：:The structure and activities of the recombination-promoting P22 Erf protein were examined in vitro. Treatment of the protein with elastase produces a stable amino-terminal fragment, consisting of amino acid residues 1 to (approximately) 136. We have purified this fragment, designated fragment B, to apparent homogeneit...

journal_title：Journal of molecular biology

authors： Poteete AR,Sauer RT,Hendrix RW

更新日期：1983-12-25 00:00:00

abstract：:The mammalian Sec61 complex forms a protein translocation channel whose function depends upon its interaction with the ribosome and with membrane proteins of the endoplasmic reticulum (ER). To study these interactions, we determined structures of "native" ribosome-channel complexes derived from ER membranes. We find t...

journal_title：Journal of molecular biology

authors： Ménétret JF,Hegde RS,Heinrich SU,Chandramouli P,Ludtke SJ,Rapoport TA,Akey CW

更新日期：2005-04-29 00:00:00

abstract：:We have isolated and characterized the murine genomic and complementary DNAs encoding erythropoietin (Epo) receptor from Epo-responsive and unresponsive mouse erythroleukemia cells. Two classes of Epo receptor cDNAs were isolated from Epo-responsive cells. One is a 55,000 Mr membrane-bound Epo receptor, and the other ...

journal_title：Journal of molecular biology

authors： Kuramochi S,Ikawa Y,Todokoro K

更新日期：1990-12-05 00:00:00

abstract：:Tat (trans-activator) proteins are early RNA binding proteins regulating lentiviral transcription. These proteins are necessary components in the life cycle of all known lentiviruses, such as the human immunodeficiency viruses (HIV) or the equine infectious anemia virus (EIAV). Tat proteins are thus ideal targets for ...

journal_title：Journal of molecular biology

authors： Bayer P,Kraft M,Ejchart A,Westendorp M,Frank R,Rösch P

更新日期：1995-04-07 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion. Cys11, His185, Glu222 and Asp233 are the other metal ligands. Wild...

journal_title：Journal of molecular biology

authors： Wang J,Duewel HS,Stuckey JA,Woodard RW,Gatti DL

更新日期：2002-11-22 00:00:00

abstract：:Single crystals of a multienzyme complex isolated from spinach leaves, and containing RUBISCO bound to the substrate RuBP have been grown and characterized. The crystals belong to the orthorhombic space group P2(1)2(1)2 with a = 173 A, b = 134 A and c = 112 A, and contain two enzyme complex molecules in the unit cell....

journal_title：Journal of molecular biology

authors： Hosur MV,Sainis JK,Kannan KK

更新日期：1993-12-20 00:00:00

abstract：:We quantify the degree to which folding occurs along a complex landscape with structurally distinct pathways using psi-analysis in combination with a protein engineering method that identifies native, non-covalent polypeptide interactions and their relative populations at the rate-limiting step. By probing the proximi...

journal_title：Journal of molecular biology

authors： Krantz BA,Dothager RS,Sosnick TR

更新日期：2004-03-19 00:00:00

abstract：:Selenophosphate synthetase (SPS) catalyzes the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential reactive selenium donor for the formation of selenocysteine (Sec) and 2-selenouridine residues in proteins and RNAs, respectively. Many SPS are themselves Sec-containing...

journal_title：Journal of molecular biology

authors： Itoh Y,Sekine S,Matsumoto E,Akasaka R,Takemoto C,Shirouzu M,Yokoyama S

更新日期：2009-02-06 00:00:00

abstract：:The rotavirus double-layered particle (DLP) is a molecular machine that transcribes 11 genomic segments of double-stranded RNA into full-length mRNA segments during viral replication. DLPs from the human Wa strain of virus, belonging to subgroup II (SG II), possess a significantly reduced level of transcriptase activi...

journal_title：Journal of molecular biology

authors： Greig SL,Berriman JA,O'Brien JA,Taylor JA,Bellamy AR,Yeager MJ,Mitra AK

更新日期：2006-02-10 00:00:00

abstract：:Huntington's disease (HD) is an inherited progressive neurodegenerative disorder caused by a pathological CAG trinucleotide repeat expansion in the large multi-exon gene, huntingtin (HTT). Although multiple pathogenic mechanisms have been proposed for HD, there is increasing interest in the RNA processing of the HTT g...

journal_title：Journal of molecular biology

authors： Hughes AC,Mort M,Elliston L,Thomas RM,Brooks SP,Dunnett SB,Jones L

更新日期：2014-04-03 00:00:00

abstract：:The MotA protein of Escherichia coli is a component of the flagella that functions together with the MotB protein in transmembrane proton conduction. It is an integral membrane protein, with four hydrophobic segments that might traverse the membrane and two short segments that are predicted to be in the periplasm. In ...

journal_title：Journal of molecular biology

authors： Zhou J,Fazzio RT,Blair DF

更新日期：1995-08-11 00:00:00

abstract：:Many proteins are composed of independently-folded domains connected by flexible linkers. The primary sequence and length of such linkers can set the effective concentration for the tethered domains, which impacts rates of association and enzyme activity. The length of such linkers can be sensitive to environmental co...

journal_title：Journal of molecular biology

authors： Basak S,Sakia N,Dougherty L,Guo Z,Wu F,Mindlin F,Lary JW,Cole JL,Ding F,Bowen ME

更新日期：2021-01-01 00:00:00

abstract：:An analysis by electron microscopy in amorphous ice and X-ray diffraction of four types of three-dimensional crystals of adenovirus fibre is presented. Fibre from adenovirus type 2 (Ad2) crystallizes in two forms depending on whether it is native or cleaved near the N terminus at Tyr17. Fibre from Ad5 also crystallize...

journal_title：Journal of molecular biology

authors： Devaux C,Adrian M,Berthet-Colominas C,Cusack S,Jacrot B

更新日期：1990-10-20 00:00:00

abstract：:Initiation of transcription at malEp and malKp, two divergent Escherichia coli promoters, depends on the presence of both CRP, a pleiotropic activator, and MalT, the maltose regulon activator. We carried out in vivo genetic and functional analysis of these promoters and characterized their interaction with MalT and CR...

journal_title：Journal of molecular biology

authors： Raibaud O,Vidal-Ingigliardi D,Richet E

更新日期：1989-02-05 00:00:00

abstract：:The preparation of hybrid histone octamers with wheat histone H2A variants replacing chicken H2A in the chicken octamer is described. The fidelity of the reconstituted hybrid octamers was confirmed by dimethyl suberimidate cross-linking. Polyglutamic-acid-mediated assembly of these octamers on long DNA and subsequent ...

journal_title：Journal of molecular biology

authors： Lindsey GG,Orgeig S,Thompson P,Davies N,Maeder DL

更新日期：1991-04-20 00:00:00

abstract：:The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzy...

journal_title：Journal of molecular biology

authors： Lario PI,Sampson N,Vrielink A

更新日期：2003-03-07 00:00:00

abstract：:The hydrolytic endoribonuclease RNase E, which is widely distributed in bacteria and plants, plays key roles in mRNA degradation and RNA processing in Escherichia coli. The enzymatic activity of RNase E is contained within the conserved amino-terminal half of the 118 kDa protein, and the carboxy-terminal half organize...

journal_title：Journal of molecular biology

authors： Callaghan AJ,Aurikko JP,Ilag LL,Günter Grossmann J,Chandran V,Kühnel K,Poljak L,Carpousis AJ,Robinson CV,Symmons MF,Luisi BF

更新日期：2004-07-23 00:00:00

abstract：:Twenty-four structures of pyridoxal-5'-phosphate (PLP)-dependent enzymes that represent five different folds are shown to share a common recognition pattern for the phosphate group of their PLP-ligands. All atoms that interact with the phosphate group of PLP in these proteins are organized within a two-layer structure...

journal_title：Journal of molecular biology

authors： Denesyuk AI,Denessiouk KA,Korpela T,Johnson MS

更新日期：2002-02-08 00:00:00

abstract：:Set8 is the only mammalian monomethyltransferase responsible for H4K20me1, a methyl mark critical for genomic integrity of eukaryotic cells. We present here a structural model for how Set8 uses multivalent interactions to bind and methylate the nucleosome based on crystallographic and solution studies of the Set8/nucl...

journal_title：Journal of molecular biology

authors： Girish TS,McGinty RK,Tan S

更新日期：2016-04-24 00:00:00

abstract：:Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In aff...

journal_title：Journal of molecular biology

authors： Grobosky CL,Lopez JB,Rennie S,Skopelitis DJ,Wiest AT,Bingman CA,Bitto E

更新日期：2012-11-02 00:00:00

abstract：:Understanding gene regulation and its adaptive significance requires not only a detailed knowledge of individual molecular interactions that give rise to changes in gene expression but also an overview of complete genetic networks and the ways in which components within them interact. Increasingly, such studies are be...

journal_title：Journal of molecular biology

authors： Burton NA,Johnson MD,Antczak P,Robinson A,Lund PA

更新日期：2010-09-03 00:00:00

abstract：:Junonia coenia densovirus (JcDNV) belongs to the densovirus genus of the Parvoviridae family and infects the larvae of the Common Buckeye butterfly. Its capsid is icosahedral and consists of viral proteins VP1 (88 kDa), VP2 (58 kDa), VP3 (52 kDa) and VP4 (47 kDa). Each viral protein has the same C terminus but differs...

journal_title：Journal of molecular biology

authors： Bruemmer A,Scholari F,Lopez-Ferber M,Conway JF,Hewat EA

更新日期：2005-04-08 00:00:00

abstract：:The yeast a1 and alpha 2 proteins are examples of homeodomain proteins that display cell-type-specific expression. They are co-expressed in only one type of cell, the a/alpha cell, where they repress the expression of a group of target genes. Using purified proteins, we demonstrate that a1 and alpha 2 form a highly sp...

journal_title：Journal of molecular biology

authors： Goutte C,Johnson AD

更新日期：1993-10-05 00:00:00

abstract：:The structure of the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase in complex with cognate DNA was determined at 1.89 A resolution in the presence of S-adenosyl-L-homocysteine. DNA recognition and the dynamics of base-flipping were studied by site-directed mutagenesis, DNA methylation kinetics and fluorescen...

journal_title：Journal of molecular biology

authors： Horton JR,Liebert K,Bekes M,Jeltsch A,Cheng X

更新日期：2006-04-28 00:00:00

abstract：:Modulator of drug activity B (MdaB) is a putative member of the DT-diaphorase family of NAD(P)H:oxidoreductases that afford cellular protection against quinonoid compounds. While there have been extensive investigations of mammalian homologues, putative prokaryotic members of this enzyme family have received little at...

journal_title：Journal of molecular biology

authors： Adams MA,Jia Z

更新日期：2006-06-02 00:00:00

abstract：:The genes for alpha-fetoprotein and albumin arose by duplication of an ancestral gene that contained three genetic domains. These domains were generated by the triplication of a primordial genetic domain composed of five exons or subdomains. That the primordial domain itself arose by amplification of a simpler sequenc...

journal_title：Journal of molecular biology

authors： Alexander F,Young PR,Tilghman SM

更新日期：1984-02-25 00:00:00

abstract：:In the presence of AMP-PCP (beta, gamma-methyleneadenosine 5'-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arra...

journal_title：Journal of molecular biology

authors： Avolio J,Lebduska S,Satir P

更新日期：1984-03-05 00:00:00

abstract：:The kink turn (K-turn) is a common motif in RNA structure, found in many RNA species important in translation, RNA modification and splicing, and the control of gene expression. In general the K-turn comprises a three nucleotide bulge followed by trans sugar-Hoogsteen G.A pairs. The RNA adopts a tightly kinked conform...

journal_title：Journal of molecular biology

authors： Turner B,Lilley DM

更新日期：2008-08-29 00:00:00

abstract：:The conversion of specific proteins or protein fragments into insoluble, ordered fibrillar aggregates is a fundamental process in protein chemistry, biology, medicine and biotechnology. As this structural conversion seems to be a property shared by many proteins, understanding the mechanism of this process will be of ...

journal_title：Journal of molecular biology

authors： Campioni S,Mossuto MF,Torrassa S,Calloni G,de Laureto PP,Relini A,Fontana A,Chiti F

更新日期：2008-06-06 00:00:00

abstract：:Large rigid-body domain movements are critical to GroEL-mediated protein folding, especially apical domain elevation and twist associated with the formation of a folding chamber upon binding ATP and co-chaperonin GroES. Here, we have modeled the anisotropic displacements of GroEL domains from various crystallized stat...

journal_title：Journal of molecular biology

authors： Chaudhry C,Horwich AL,Brunger AT,Adams PD

更新日期：2004-09-03 00:00:00