当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Extended C-terminal tail of wheat histone H2A interacts with DNA of the "linker" region.

Extended C-terminal tail of wheat histone H2A interacts with DNA of the "linker" region.

Abstract：

:The preparation of hybrid histone octamers with wheat histone H2A variants replacing chicken H2A in the chicken octamer is described. The fidelity of the reconstituted hybrid octamers was confirmed by dimethyl suberimidate cross-linking. Polyglutamic-acid-mediated assembly of these octamers on long DNA and subsequent micrococcal nuclease (MNase) digestion demonstrated that, whereas chicken octamers protected 167 base-pairs (representing 2 full turns of DNA), hybrid histone octamers containing wheat histone H2A(1) with its 19 amino acid residue C-terminal extension protected an additional 16 base pairs of DNA against nuclease digestion. The protection observed by hybrid histone octamers containing wheat histone H2A(3) with both a 15 residue N-terminal and a 19 residue C-terminal extension was identical with that observed with H2A(1)-containing hybrid histone octamers with only the 19 residue C-terminal extension. These results suggest that the role of the C-terminal extension is to bind to DNA of the "linker" region. The thermal denaturation of chicken and hybrid core particles was identical in 10 mM-Tris.HCl.20 mM-NaCl, 0.1 mM-EDTA, confirming that there was no interaction between the basic C-terminal extension and DNA of the core particle. Denaturation in EDTA, however, showed that hybrid core particles had enhanced stability, suggesting that the known conformational change of core particles at very low ionic strength allows the C-terminal extension to bind to core particle DNA under these conditions. A model accounting for the observed MNase protection is presented.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lindsey GG,Orgeig S,Thompson P,Davies N,Maeder DL

doi

10.1016/0022-2836(91)90268-b

subject

Has Abstract

pub_date

1991-04-20 00:00:00

pages

805-13

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)90268-B

journal_volume

218

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Crystal structure of Hsc20, a J-type Co-chaperone from Escherichia coli.

    abstract：:Hsc20 is a 20 kDa J-protein that regulates the ATPase activity and peptide-binding specificity of Hsc66, an hsp70-class molecular chaperone. We report herein the crystal structure of Hsc20 from Escherichia coli determined to a resolution of 1.8 A using a combination of single isomorphous replacement (SIR) and multi-wa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4252

    authors： Cupp-Vickery JR,Vickery LE

    更新日期：2000-12-15 00:00:00

  • Contribution of the hydrophobic effect to globular protein stability.

    abstract：:The decrease in conformational stability, delta(delta G), has been measured for 72 aliphatic side-chain mutants from four proteins in which a larger side-chain is replaced by a smaller side-chain so that steric effects are minimal. When these delta(delta G) values are corrected to the same accessibility, namely 100% b...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90121-y

    authors： Pace CN

    更新日期：1992-07-05 00:00:00

  • Modifying Chromatin by Histone Tail Clipping.

    abstract：:Post-translational modifications (PTMs) of histone proteins play a crucial role in the regulation of chromatin structure and functions. Studies in the last few decades have revealed the significance of histone PTMs in key cellular processes including DNA replication, repair, transcription, apoptosis and cell cycle reg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.07.013

    authors： Azad GK,Swagatika S,Kumawat M,Kumawat R,Tomar RS

    更新日期：2018-09-14 00:00:00

  • ncEP: A Manually Curated Database for Experimentally Validated ncRNA-encoded Proteins or Peptides.

    abstract：:Noncoding RNAs (ncRNAs), such as lncRNAs, circRNAs and pri-miRNAs, play important roles in physiological and pathological processes. Recently, it was demonstrated that they could encode proteins or peptides. However, relevant information is scattered across numerous published articles, which is inconvenient for the ex...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.02.022

    authors： Liu H,Zhou X,Yuan M,Zhou S,Huang YE,Hou F,Song X,Wang L,Jiang W

    更新日期：2020-05-15 00:00:00

  • The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex.

    abstract：:Cre recombinase uses two pairs of sequential cleavage and religation reactions to exchange homologous DNA strands between 34 base-pair (bp) LoxP recognition sequences. In the oligomeric recombination complex, a switch between "cleaving" and "non-cleaving" subunit conformations regulates the number, order, and regio-sp...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00246-2

    authors： Martin SS,Pulido E,Chu VC,Lechner TS,Baldwin EP

    更新日期：2002-05-24 00:00:00

  • Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain.

    abstract：:ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anth...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.11.013

    authors： Waterman DG,Ortiz-Lombardía M,Fogg MJ,Koonin EV,Antson AA

    更新日期：2006-02-10 00:00:00

  • Are the molten globule and the unfolded states of apo-alpha-lactalbumin enthalpically equivalent?

    abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1364

    authors： Xie D,Bhakuni V,Freire E

    更新日期：1993-07-05 00:00:00

  • Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy.

    abstract：:Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality. Here, we have identified a protein encoded in the Arabidopsis genome ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.12.066

    authors： Grasser M,Kane CM,Merkle T,Melzer M,Emmersen J,Grasser KD

    更新日期：2009-02-27 00:00:00

  • Direct Homologous dsDNA-dsDNA Pairing: How, Where, and Why?

    abstract：:The ability of homologous chromosomes (or selected chromosomal loci) to pair specifically in the apparent absence of DNA breakage and recombination represents a prominent feature of eukaryotic biology. The mechanism of homology recognition at the basis of such recombination-independent pairing has remained elusive. A ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.11.005

    authors： Mazur AK,Nguyen TS,Gladyshev E

    更新日期：2020-02-07 00:00:00

  • X-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution.

    abstract：:The rotavirus inner capsid particle, known as the "double-layered particle" (DLP), is the "payload" delivered into a cell in the process of viral infection. Its inner and outer protein layers, composed of viral protein (VP) 2 and VP6, respectively, package the 11 segments of the double-stranded RNA (dsRNA) of the vira...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.01.055

    authors： McClain B,Settembre E,Temple BR,Bellamy AR,Harrison SC

    更新日期：2010-03-26 00:00:00

  • Structure, evolutionary conservation, and conformational dynamics of Homo sapiens fascin-1, an F-actin crosslinking protein.

    abstract：:Eukaryotes have several highly conserved actin-binding proteins that crosslink filamentous actin into compact ordered bundles present in distinct cytoskeletal processes, including microvilli, stereocilia and filopodia. Fascin is an actin-binding protein that is present predominantly in filopodia, which are believed to...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.04.043

    authors： Sedeh RS,Fedorov AA,Fedorov EV,Ono S,Matsumura F,Almo SC,Bathe M

    更新日期：2010-07-16 00:00:00

  • Context-dependent remodeling of structure in two large protein fragments.

    abstract：:Protein folding involves the formation of secondary structural elements from the primary sequence and their association with tertiary assemblies. The relation of this primary sequence to a specific folded protein structure remains a central question in structural biology. An increasing body of evidence suggests that v...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.08.020

    authors： Schellenberg MJ,Ritchie DB,Wu T,Markin CJ,Spyracopoulos L,MacMillan AM

    更新日期：2010-10-01 00:00:00

  • Recycling of ribosomal complexes stalled at the step of elongation in Escherichia coli.

    abstract：:Translating ribosomes often stall during elongation. The stalled ribosomes are known to be recycled by tmRNA (SsrA)-mediated trans-translation. Another process that recycles the stalled ribosomes is characterized by peptidyl-tRNA release. However, the mechanism of peptidyl-tRNA release from the stalled ribosomes is no...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.033

    authors： Singh NS,Ahmad R,Sangeetha R,Varshney U

    更新日期：2008-07-11 00:00:00

  • Preliminary crystallographic analysis of the breakage-reunion domain of the Escherichia coli DNA gyrase A protein.

    abstract：:The 64 x 10(3) Mr N-terminal breakage-reunion domain of the Escherichia coli DNA gyrase A protein was purified from an over-expressing strain. When complexed with the gyrase B protein, this truncated A protein has all of the enzymic properties of the full-length counterpart, although with reduced efficiency in some ca...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(05)80162-7

    authors： Reece RJ,Dauter Z,Wilson KS,Maxwell A,Wigley DB

    更新日期：1990-10-20 00:00:00

  • Weak and strong states of kinesin and ncd.

    abstract：:Kinesin superfamily molecular motors step along microtubules (MTs) via a cycle of conformational changes which is coupled to ATP turnover. To probe the coupling mechanism, we titrated the effects of various nucleotides on MT binding by two superfamily members; MT plus-end-directed kinesin and MT minus-end-directed non...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0147

    authors： Crevel IM,Lockhart A,Cross RA

    更新日期：1996-03-22 00:00:00

  • Functional analysis of Box II mutations in yeast site-specific recombinases Flp and R. Significance of amino acid conservation within the Int family and the yeast sub-family.

    abstract：:The site-specific recombinases Flp and R from Saccharomyces cerevisiae and Zygosaccharomyces rouxii, respectively, are related proteins that share approximately 30% amino acid matches. They exhibit a common reaction mechanism that appears to be conserved within the larger Integrase family of site-specific recombinases...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90317-d

    authors： Lee J,Serre MC,Yang SH,Whang I,Araki H,Oshima Y,Jayaram M

    更新日期：1992-12-20 00:00:00

  • Inter-domain motions of the N-domain of the KdpFABC complex, a P-type ATPase, are not driven by ATP-induced conformational changes.

    abstract：:P-type ATPases are involved in the active transport of ions across biological membranes. The KdpFABC complex (P-type ATPase) of Escherichia coli is a high-affinity K+ uptake system that operates only when the cell experiences osmotic stress or K+ limitation. Here, we present the solution structure of the nucleotide bi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.07.060

    authors： Haupt M,Bramkamp M,Coles M,Altendorf K,Kessler H

    更新日期：2004-10-01 00:00:00

  • Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity.

    abstract：:Deoxyribonucleoside kinases (dNKs) catalyze the transfer of a phosphoryl group from ATP to a deoxyribonucleoside (dN), a key step in DNA precursor synthesis. Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known. Here we report the structure of such ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.12.010

    authors： Welin M,Wang L,Eriksson S,Eklund H

    更新日期：2007-03-09 00:00:00

  • Rescuing a destabilized protein fold through backbone cyclization.

    abstract：:We describe the physicochemical characterization of various circular and linear forms of the approximately 60 residue N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein. Structural, dynamic, thermodynamic, kinetic and biochemical studies reveal that backbone circularization does not prevent t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4631

    authors： Camarero JA,Fushman D,Sato S,Giriat I,Cowburn D,Raleigh DP,Muir TW

    更新日期：2001-05-18 00:00:00

  • Refined crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.0 A resolution.

    abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90527-d

    authors： Herzberg O

    更新日期：1991-02-20 00:00:00

  • Differential expression of two clusters of mouse histone genes.

    abstract：:The mouse histone mRNAs coded for by three different cloned DNA fragments have been characterized. Two of these cloned DNA fragments, MM221 and MM291, located on chromosome 13, code for H3, H2b and H2a histone mRNAs, which are expressed at low levels in cultured mouse cells and fetal mice. The other DNA fragment, MM61...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90211-6

    authors： Graves RA,Wellman SE,Chiu IM,Marzluff WF

    更新日期：1985-05-25 00:00:00

  • Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII.

    abstract：:We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.02.074

    authors： Townson SA,Samuelson JC,Vanamee ES,Edwards TA,Escalante CR,Xu SY,Aggarwal AK

    更新日期：2004-05-07 00:00:00

  • Crystallization and preliminary crystallographic data of a Streptomyces scabies extracellular esterase.

    abstract：:X-ray quality single crystals of an extracellular esterase from pathogenic Streptomyces scabies were obtained by the hanging drop method. The crystals are monoclinic (space group C2, a = 161.1 A, b = 51.2 A, c = 124.2 A, beta = 100.6 degrees) with two molecules related by a noncrystallographic dyad in the asymmetric u...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90908-3

    authors： Green R,Schottel JL,Swenson L,Wei Y,Derewenda ZS

    更新日期：1992-09-20 00:00:00

  • Structural changes in GroEL effected by binding a denatured protein substrate.

    abstract：:In the absence of nucleotides or cofactors, the Escherichia coli chaperonin GroEL binds select proteins in non-native conformations, such as denatured glutamine synthetase (GS) monomers, preventing their aggregation and spontaneous renaturation. The nature of the GroEL-GS complexes thus formed, specifically the effect...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4613

    authors： Falke S,Fisher MT,Gogol EP

    更新日期：2001-05-11 00:00:00

  • Two amyloid States of the prion protein display significantly different folding patterns.

    abstract：:It has been well established that a single amino acid sequence can give rise to several conformationally distinct amyloid states. The extent to which amyloid structures formed within the same sequence are different, however, remains unclear. To address this question, we studied two amyloid states (referred to as R- an...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.05.051

    authors： Ostapchenko VG,Sawaya MR,Makarava N,Savtchenko R,Nilsson KP,Eisenberg D,Baskakov IV

    更新日期：2010-07-23 00:00:00

  • Tunicate muscle actin genes. Structure and organization as a gene cluster.

    abstract：:We have isolated and determined the complete nucleotide sequences of two genes, HrMA4a and HrMA2, which encode the same muscle actin protein of the tunicate Halocynthia roretzi. HrMA4a and HrMA2 contain three exons, and the genes have intron-exon splice junctions at the same positions. The 5' flanking region of HrMA4a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90237-e

    authors： Kusakabe T,Makabe KW,Satoh N

    更新日期：1992-10-05 00:00:00

  • Molecular model of an alpha-helical prion protein dimer and its monomeric subunits as derived from chemical cross-linking and molecular modeling calculations.

    abstract：:Prions are the agents of a series of lethal neurodegenerative diseases. They are composed largely, if not entirely, of the host-encoded prion protein (PrP), which can exist in the cellular isoform PrP(C) and the pathological isoform PrP(Sc). The conformational change of the alpha-helical PrP(C) into beta-sheet-rich Pr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.11.035

    authors： Kaimann T,Metzger S,Kuhlmann K,Brandt B,Birkmann E,Höltje HD,Riesner D

    更新日期：2008-02-15 00:00:00

  • Human cardiac myosin binding protein C: structural flexibility within an extended modular architecture.

    abstract：:New insights into the modular organization and flexibility of the N-terminal half of human cardiac myosin binding protein C (cMyBP-C) and information on the association state of the full-length protein have been deduced from a combined small-angle X-ray scattering (SAXS) and NMR study. SAXS data show that the first fi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.10.029

    authors： Jeffries CM,Lu Y,Hynson RM,Taylor JE,Ballesteros M,Kwan AH,Trewhella J

    更新日期：2011-12-16 00:00:00

  • A method for helical RNA global structure determination in solution using small-angle x-ray scattering and NMR measurements.

    abstract：:We report a "top-down" method that uses mainly duplexes' global orientations and overall molecular dimension and shape restraints, which were extracted from experimental NMR and small-angle X-ray scattering data, respectively, to determine global architectures of RNA molecules consisting of mostly A-form-like duplexes...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.08.001

    authors： Wang J,Zuo X,Yu P,Xu H,Starich MR,Tiede DM,Shapiro BA,Schwieters CD,Wang YX

    更新日期：2009-10-30 00:00:00

  • Principles governing amino acid composition of integral membrane proteins: application to topology prediction.

    abstract：:A new method is suggested here for topology prediction of helical transmembrane proteins. The method is based on the hypothesis that the localizations of the transmembrane segments and the topology are determined by the difference in the amino acid distributions in various structural parts of these proteins rather tha...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2107

    authors： Tusnády GE,Simon I

    更新日期：1998-10-23 00:00:00