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Dynein arm substructure and the orientation of arm-microtubule attachments.

Abstract：

:In the presence of AMP-PCP (beta, gamma-methyleneadenosine 5'-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arrangement of these subunits makes it possible to distinguish A from B subfiber binding sites on a single arm and to demonstrate that the head of an extended arm on subfiber A of one ciliary doublet is capable of binding to subfiber B of an adjacent doublet in a specific orientation, which supports a key step in a current model of the mechanochemical cycle by which the arm produces microtubule sliding in the ciliary axoneme.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Avolio J,Lebduska S,Satir P

doi

10.1016/0022-2836(84)90127-x

subject

Has Abstract

pub_date

1984-03-05 00:00:00

pages

389-401

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(84)90127-X

journal_volume

173

pub_type

杂志文章

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