Abstract:
:Integrins, which are heterodimeric (α and β subunits) signal-transducer proteins, are essential for cell adhesion and migration. β cytosolic tails (β-CTs) of integrins interact with a number of cytosolic proteins including talin, Dok1, and 14-3-3ζ. The formation of multiprotein complexes with β-CTs is involved in the activation and regulation of integrins. The leukocyte-specific β2 integrins are essential for leukocyte trafficking, phagocytosis, antigen presentation, and proliferation. In this study, we examined the binding interactions between integrin β2-CT and T758-phosphorylated β2-CT with positive regulators talin and 14-3-3ζ and negative regulator Dok1. Residues of the F3 domain of talin belonging to the C-terminal helix, β-strand 5, and the adjacent loop were found to be involved in the binding interactions with β2-CT. The binding affinity between talin F3 and β2-CT was reduced when β2 T758 was phosphorylated, but this modification promoted 14-3-3ζ binding. However, we were able to detect stable ternary complex formation of T758-phosphorylated β2-CT, talin F3, and 14-3-3ζ that involved the repositioning of talin F3 on β2-CT. We showed that Dok1 binding to β2-CT was reduced in the presence of 14-3-3ζ and when β2 T758 was phosphorylated. Based on these data, we propose a sequential model of β2 integrin activation involving these molecules. Our study provides for the first time insights toward β2 integrin activation that involves a multiprotein complex.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Chatterjee D,Zhiping LL,Tan SM,Bhattacharjya Sdoi
10.1016/j.jmb.2016.08.014subject
Has Abstractpub_date
2016-10-09 00:00:00pages
4129-4142issue
20eissn
0022-2836issn
1089-8638pii
S0022-2836(16)30309-6journal_volume
428pub_type
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