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Crystallization and preliminary crystallographic analysis of a DNA (cytosine-5)-methyltransferase from Haemophilus aegyptius bound covalently to DNA.

Abstract：

:A DNA (cytosine)-5-methyltransferase from Haemophilus aegyptius (M.Hae III), which catalyzes methyl transfer from S-adenosyl-L-methionine to DNA, has been crystallized as a covalent complex with a suicide oligonucleotide substrate. Crystals of the co-complex were grown by vapor diffusion with hanging droplets, using polyethylene glycol 3500 as the precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1); the unit cell parameters are a = 57.6 A, b = 108.0 A, c = 155.8 A with two protein-DNA complexes in the asymmetric unit. Complete sets of native and derivative data have been collected to 2.7 A using a laboratory source.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Reinisch KM,Chen L,Verdine GL,Lipscomb WN

doi

10.1006/jmbi.1994.1319

subject

Has Abstract

pub_date

1994-05-13 00:00:00

pages

626-9

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(84)71319-2

journal_volume

238

pub_type

杂志文章

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