当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Ancylostoma ceylanicum excretory-secretory protein 2 adopts a netrin-like fold and defines a novel family of nematode proteins.

Ancylostoma ceylanicum excretory-secretory protein 2 adopts a netrin-like fold and defines a novel family of nematode proteins.

Abstract：

:Hookworms are human parasites that have devastating effects on global health, particularly in underdeveloped countries. Ancylostoma ceylanicum infects humans and animals, making it a useful model organism to study disease pathogenesis. A. ceylanicum excretory-secretory protein 2 (AceES-2), a highly immunoreactive molecule secreted by adult worms at the site of intestinal attachment, is partially protective when administered as a mucosal vaccine against hookworm anemia. The crystal structure of AceES-2 determined at 1.75 Å resolution shows that it adopts a netrin-like fold similar to that found in tissue inhibitors of matrix metalloproteases (TIMPs) and in complement factors C3 and C5. However, recombinant AceES-2 does not significantly inhibit the 10 most abundant human matrix metalloproteases or complement-mediated cell lysis. The presence of a highly acidic surface on AceES-2 suggests that it may function as a cytokine decoy receptor. Several small nematode proteins that have been annotated as TIMPs or netrin-domain-containing proteins display sequence homology in structurally important regions of AceES-2's netrin-like fold. Together, our results suggest that AceES-2 defines a novel family of nematode netrin-like proteins, which may function to modulate the host immune response to hookworm and other parasites.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kucera K,Harrison LM,Cappello M,Modis Y

doi

10.1016/j.jmb.2011.02.033

subject

Has Abstract

pub_date

2011-04-22 00:00:00

pages

9-17

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(11)00190-2

journal_volume

408

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D.

    abstract：:Pulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D mo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2018.03.027

    authors： Arroyo R,Martín-González A,Echaide M,Jain A,Brondyk WH,Rosenbaum J,Moreno-Herrero F,Pérez-Gil J

    更新日期：2018-05-11 00:00:00

  • CryptoSite: Expanding the Druggable Proteome by Characterization and Prediction of Cryptic Binding Sites.

    abstract：:Many proteins have small-molecule binding pockets that are not easily detectable in the ligand-free structures. These cryptic sites require a conformational change to become apparent; a cryptic site can therefore be defined as a site that forms a pocket in a holo structure, but not in the apo structure. Because many p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.01.029

    authors： Cimermancic P,Weinkam P,Rettenmaier TJ,Bichmann L,Keedy DA,Woldeyes RA,Schneidman-Duhovny D,Demerdash ON,Mitchell JC,Wells JA,Fraser JS,Sali A

    更新日期：2016-02-22 00:00:00

  • Regulation of Mitochondrial Electron Transport Chain Assembly.

    abstract：:Mitochondrial function depends on the correct synthesis, transport, and assembly of proteins and cofactors of the electron transport chain. The initial idea that the respiratory chain protein complexes (RCCs) were independent structures in the inner mitochondrial membrane evolved after the identification of higher qua...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.09.016

    authors： Cogliati S,Lorenzi I,Rigoni G,Caicci F,Soriano ME

    更新日期：2018-12-07 00:00:00

  • Characterization of hnRNP K protein-RNA interactions.

    abstract：:The heterogeneous nuclear ribonucleoprotein K protein is an RNA-binding protein found in several subcellular compartments where it is thought to be involved in signaling multiple processes that compose gene expression. K protein contains three K homology (KH) domains that mediate RNA-binding. We used a serial analysis...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.07.099

    authors： Klimek-Tomczak K,Wyrwicz LS,Jain S,Bomsztyk K,Ostrowski J

    更新日期：2004-09-24 00:00:00

  • Crystal and molecular structure of human annexin V after refinement. Implications for structure, membrane binding and ion channel formation of the annexin family of proteins.

    abstract：:Two crystal forms (P6(3) and R3) of human annexin V have been crystallographically refined at 2.3 A and 2.0 A resolution to R-values of 0.184 and 0.174, respectively, applying very tight stereochemical restraints with deviations from ideal geometry of 0.01 A and 2 degrees. The three independent molecules (2 in P6(3), ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90984-r

    authors： Huber R,Berendes R,Burger A,Schneider M,Karshikov A,Luecke H,Römisch J,Paques E

    更新日期：1992-02-05 00:00:00

  • The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase.

    abstract：:Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00624-1

    authors： Atzenhofer W,Regelsberger G,Jacob U,Peschek G,Furtmüller P,Huber R,Obinger C

    更新日期：2002-08-16 00:00:00

  • An unusually long-lived antisense RNA in plasmid copy number control: in vivo RNAs encoded by the streptococcal plasmid pIP501.

    abstract：:The main regulator of pIP501 replication is an antisense RNA (RNAIII) that induces transcriptional attenuation of the essential RNAII. Previous studies identified the termination point in vivo and demonstrated attenuation in vitro. This in vivo analysis confirms the appearance of attenuated RNAII dependent on RNAIII. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0023

    authors： Brantl S,Wagner EG

    更新日期：1996-01-19 00:00:00

  • Architecture of the ribosome-channel complex derived from native membranes.

    abstract：:The mammalian Sec61 complex forms a protein translocation channel whose function depends upon its interaction with the ribosome and with membrane proteins of the endoplasmic reticulum (ER). To study these interactions, we determined structures of "native" ribosome-channel complexes derived from ER membranes. We find t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.02.053

    authors： Ménétret JF,Hegde RS,Heinrich SU,Chandramouli P,Ludtke SJ,Rapoport TA,Akey CW

    更新日期：2005-04-29 00:00:00

  • A new model for Schizosaccharomyces pombe telomere recognition: the telomeric single-stranded DNA-binding activity of Pot11-389.

    abstract：:The protection of telomeres 1 (Pot1) proteins specifically recognize the single-stranded 3' end of the telomere, an activity essential for sustained cellular viability and proliferation. The current model for the telomeric single-stranded DNA (ssDNA) binding activity of Schizosaccharomyces pombe Pot1 is based on a 20 ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.06.002

    authors： Croy JE,Podell ER,Wuttke DS

    更新日期：2006-08-04 00:00:00

  • Isolation and characterization of ribosomal DNA variants from Sciara coprophila.

    abstract：:The ribosomal RNA multigene family in the fungus fly Sciara coprophila contains a total of only 65 to 70 repeat units. We explored the types and frequencies of variant repeats in this small multigene family by characterizing different cloned rDNA variants from Sciara. Although we did not observe any intergenic spacer ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90286-6

    authors： Kerrebrock AW,Srivastava R,Gerbi SA

    更新日期：1989-11-05 00:00:00

  • Use of site-specific recombination as a probe of DNA structure and metabolism in vivo.

    abstract：:We used site-specific recombination catalyzed by the bacteriophage lambda Int system to probe DNA structure and metabolism in vivo. In vitro, the complexity of catenated products was linearly proportional to substrate supercoil density. A system was developed that gave efficient, controlled Int recombination in Escher...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90369-x

    authors： Bliska JB,Cozzarelli NR

    更新日期：1987-03-20 00:00:00

  • Nucleotide sequences of immunoglobulin-epsilon pseudogenes in man and apes and their phylogenetic relationships.

    abstract：:To understand the phylogenetic relationships between hominoids, the nucleotide sequences of immunoglobulin-epsilon processed pseudogenes from chimpanzee, gorilla and orangutan were determined. The basic structures of these processed pseudogenes agreed with their human counterpart. Although the degrees of nucleotide di...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90366-5

    authors： Ueda S,Watanabe Y,Saitou N,Omoto K,Hayashida H,Miyata T,Hisajima H,Honjo T

    更新日期：1989-01-05 00:00:00

  • Impact of pro segments on the folding and function of human neutrophil alpha-defensins.

    abstract：:Human neutrophil alpha-defensins (HNPs) are synthesized in vivo as inactive precursor proteins, i.e. preproHNPs. A series of sequential proteolytic events excise the N-terminal inhibitory pro peptide, leading to defensin maturation and storage in azurophilic granules. The anionic pro peptide, required for correct sub-...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.02.040

    authors： Wu Z,Li X,Ericksen B,de Leeuw E,Zou G,Zeng P,Xie C,Li C,Lubkowski J,Lu WY,Lu W

    更新日期：2007-04-27 00:00:00

  • Structure of short thick filaments from Limulus muscle.

    abstract：:Shortened Limulus thick filaments, isolated from stimulated muscle, are structurally similar to long filaments, isolated from unstimulated muscle, except for length. Both have 3-fold screw symmetry with a helical repeat at approximately 43 nm, axial spacing of 14.5 nm between successive crowns of crossbridges and 4-fo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90351-1

    authors： Levine RJ,Kensler RW

    更新日期：1985-03-20 00:00:00

  • DNA topology and geometry in Flp and Cre recombination.

    abstract：:The Flp recombinase of yeast and the Cre recombinase of bacteriophage P1 both belong to the lambda-integrase (Int) family of site-specific recombinases. These recombination systems recognize recombination-target sequences that consist of two 13bp inverted repeats flanking a 6 or 8bp spacer sequence. Recombination reac...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.01.037

    authors： Vetcher AA,Lushnikov AY,Navarra-Madsen J,Scharein RG,Lyubchenko YL,Darcy IK,Levene SD

    更新日期：2006-04-07 00:00:00

  • Determining the Limitations and Benefits of Noise in Gene Regulation and Signal Transduction through Single Cell, Microscopy-Based Analysis.

    abstract：:Stochastic fluctuations, termed "noise," in the level of biological molecules can greatly impact cellular functions. While biological noise can sometimes be detrimental, recent studies have provided an increasing number of examples in which biological noise can be functionally beneficial. Rather than provide an exhaus...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2017.03.007

    authors： Harton MD,Batchelor E

    更新日期：2017-04-21 00:00:00

  • pK values of histidine residues in ribonuclease Sa: effect of salt and net charge.

    abstract：:The primary goal of this study was to gain a better understanding of the effect of environment and ionic strength on the pK values of histidine residues in proteins. The salt-dependence of pK values for two histidine residues in ribonuclease Sa (RNase Sa) (pI=3.5) and a variant in which five acidic amino acids have be...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01274-3

    authors： Huyghues-Despointes BM,Thurlkill RL,Daily MD,Schell D,Briggs JM,Antosiewicz JM,Pace CN,Scholtz JM

    更新日期：2003-01-31 00:00:00

  • Two-dimensional crystal packing of matrix porin. A channel forming protein in Escherichia coli outer membranes.

    abstract：:Two-dimensional crystalline porin sheets were obtained by reconstitution of monodisperse protein trimers and phospholipids (dimyristoylphosphatidylcholine) by detergent dialysis, analogous to the reconstitution method used for functional tests (Schindler & Rosenbusch, 1981). Three different packing arrangements were o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(83)80275-7

    authors： Dorset DL,Engel A,Häner M,Massalski A,Rosenbusch JP

    更新日期：1983-04-25 00:00:00

  • Brujita Integrase: A Simple, Arm-Less, Directionless, and Promiscuous Tyrosine Integrase System.

    abstract：:Mycobacteriophage Brujita is an unusual temperate phage in which establishment of superinfection immunity is dependent on chromosomal integration. Integration is mediated by a non-canonical tyrosine integrase (Int) lacking an N-terminal domain typically associated with binding to arm-type sites within the phage attach...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.04.023

    authors： Lunt BL,Hatfull GF

    更新日期：2016-06-05 00:00:00

  • Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene.

    abstract：:G protein coupled receptors (GPCRs) are integral membrane proteins that mediate cellular responses to a wide variety of extracellular signals. However, the structural basis for activation of this class of receptors by ligand binding is not well understood. We report here the use of a systematic genetic protocol for id...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0816

    authors： Sommers CM,Dumont ME

    更新日期：1997-02-28 00:00:00

  • Non-receptor tyrosine kinases c-Abl and Arg regulate the activity of C/EBPbeta.

    abstract：:The CCAAT/enhancer-binding protein beta (C/EBPbeta) is a critical transcription factor that regulates gene expression during numerous biological processes, including differentiation, metabolism, homeostasis, proliferation, tumorigenesis, inflammation, and apoptosis. In this study, interactions between C/EBPbeta and ei...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.06.055

    authors： Li X,Liu X,Wang G,Zhu X,Qu X,Li X,Yang Y,Peng L,Li C,Li P,Huang W,Ma Q,Cao C

    更新日期：2009-08-28 00:00:00

  • Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein.

    abstract：:The NusA transcription elongation protein, which binds RNA, contains sequences corresponding to the S1 and KH classes of identified RNA binding domains. An essential function in E. coli, NusA is also one of the host factors required for action of the N transcription antitermination protein of lambda. Tandem KH domains...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00238-3

    authors： Zhou Y,Mah TF,Greenblatt J,Friedman DI

    更新日期：2002-05-17 00:00:00

  • High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds.

    abstract：:Proteinaceous serine proteinase inhibitors are widespread throughout the plant kingdom where they play an important role in protection against pests and pathogens. Here, we describe the isolation and characterisation of a novel 14 amino acid residue cyclic peptide from sunflower seeds, which is a potent inhibitor of t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2891

    authors： Luckett S,Garcia RS,Barker JJ,Konarev AV,Shewry PR,Clarke AR,Brady RL

    更新日期：1999-07-09 00:00:00

  • Are the molten globule and the unfolded states of apo-alpha-lactalbumin enthalpically equivalent?

    abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1364

    authors： Xie D,Bhakuni V,Freire E

    更新日期：1993-07-05 00:00:00

  • Autoinhibition of ETV6 (TEL) DNA binding: appended helices sterically block the ETS domain.

    abstract：:ETV6 (or TEL), a transcriptional repressor belonging to the ETS family, is frequently involved in chromosomal translocations linked with human cancers. It displays a DNA-binding mode distinct from other ETS proteins due to the presence of a self-associating PNT domain. In this study, we used NMR spectroscopy to dissec...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.05.010

    authors： Coyne HJ 3rd,De S,Okon M,Green SM,Bhachech N,Graves BJ,McIntosh LP

    更新日期：2012-08-03 00:00:00

  • Static disorder and librational motions of the purine bases in films of oriented Li-DNA.

    abstract：:Solid-state 2H nuclear magnetic resonance line shapes have been obtained from folded films of oriented Li-DNA molecules with the purine bases selectively labeled with deuterium at the 8-position. From line shape simulations, the static base tilts as well as the anisotropic motional amplitudes were determined as a func...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90461-5

    authors： Brandes R,Vold RR,Kearns DR,Rupprecht A

    更新日期：1988-07-20 00:00:00

  • Use of binding site neighbor-effect parameters to evaluate the interactions between adjacent ligands on a linear lattice. Effects on ligand-lattice association.

    abstract：:A method using binding site "neighbor-effect" parameters (NEPs) is introduced to evaluate the effects of interaction between adjacent ligands on their binding to an infinite linear lattice. Binding site overlap is also taken into account. This enables the conditional probability approach of McGhee & von Hippel to be e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90262-i

    authors： Wolfe AR,Meehan T

    更新日期：1992-02-20 00:00:00

  • Molecular evolution of multisubunit RNA polymerases: sequence analysis.

    abstract：:Transcription in all cellular organisms is performed by multisubunit, DNA-dependent RNA polymerases that synthesize RNA from DNA templates. Previous sequence and structural studies have elucidated the importance of shared regions common to all multisubunit RNA polymerases. In addition, RNA polymerases contain multiple...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.10.062

    authors： Lane WJ,Darst SA

    更新日期：2010-01-29 00:00:00

  • A group of repetitive human DNA families that is characterized by extrachromosomal oligomers and restriction-fragment length polymorphism.

    abstract：:We have recently reported a novel human repetitive DNA (Sau3A family) that exists both in the chromosomes and in the extrachromosomal fraction. Several more clones that hybridized with the Sau3A family were isolated from the extrachromosomal fraction of HeLa cells. Use of these clones as probes has revealed that at le...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90342-1

    authors： Kiyama R,Matsui H,Okumura K,Oishi M

    更新日期：1987-02-20 00:00:00

  • CTL recognition of a protective immunodominant influenza A virus nucleoprotein epitope utilizes a highly restricted Vbeta but diverse Valpha repertoire: functional and structural implications.

    abstract：:To investigate protective immunity conferred by CTL against viral pathogens, we have analyzed CD8(+) T cell responses to the immunodominant nucleoprotein epitope (NP(366-374)) of influenza A virus in B6 mice during primary and secondary infections in vivo. Unlike the highly biased TCR Vbeta repertoire, the associated ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.06.057

    authors： Zhong W,Dixit SB,Mallis RJ,Arthanari H,Lugovskoy AA,Beveridge DL,Wagner G,Reinherz EL

    更新日期：2007-09-14 00:00:00