Abstract:
:Complex gene regulation systems ensure the maintenance of cellular identity during early development in mammals. Eukaryotic small RNAs have emerged as critical players in RNA interference (RNAi) by mediating gene silencing during embryonic stem cell self-renewal. Most of the proteins involved in the biogenesis of small RNAs are essential for proliferation and differentiation into the three germ layers of mouse embryonic stem cells. In the last decade, new functions for some RNAi proteins, independent of their roles in RNAi pathways, have been demonstrated in different biological systems. In parallel, new concepts in stem cell biology have emerged. Here, we review and integrate the current understanding of how RNAi proteins regulate stem cell identity with the new advances in the stem cell field and the recent non-canonical functions of the RNAi proteins. Finally, we propose a reevaluation of all RNAi mutant phenotypes, as non-canonical (small non-coding RNA independent) functions may contribute to the molecular mechanisms governing mouse embryonic stem cells commitment.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Bodak M,Cirera-Salinas D,Luitz J,Ciaudo Cdoi
10.1016/j.jmb.2017.01.014subject
Has Abstractpub_date
2017-05-19 00:00:00pages
1532-1543issue
10eissn
0022-2836issn
1089-8638pii
S0022-2836(17)30042-6journal_volume
429pub_type
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