Crystallization and preliminary X-ray studies of a protease from Pseudomonas aeruginosa.

abstract：:We have conducted a detailed kinetic and thermodynamic analysis of open complex formation between Escherichia coli RNA polymerase and the A1 promoter from bacteriophage T7 by monitoring alterations in the intrinsic protein fluorescence of RNA polymerase in stopped-flow kinetic studies. The stopped-flow kinetic data ar...

journal_title：Journal of molecular biology

authors： Johnson RS,Chester RE

更新日期：1998-10-23 00:00:00

abstract：:The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to mo...

journal_title：Journal of molecular biology

authors： Bujalowski W,Lohman TM

更新日期：1991-01-05 00:00:00

abstract：:Tafazzin is a mitochondrial enzyme that exchanges fatty acids between phospholipids by phospholipid-lysophospholipid transacylation. The reaction alters the molecular species composition and, as a result, the physical properties of lipids. In vivo, the most important substrate of tafazzin is the mitochondria-specific ...

journal_title：Journal of molecular biology

authors： Schlame M,Xu Y

更新日期：2020-08-21 00:00:00

abstract：:We identified the predominant 5' ends of an mRNA in Escherichia coli to the exact nucleotides. There are four such ends of lac mRNA in fully induced cells. About 70% of the molecules have the reported major in vitro end, A-A-U-U-G (at +1), which is located 38 nucleotides before the A-U-G translation start. Another 15%...

journal_title：Journal of molecular biology

authors： Cannistraro VJ,Kennell D

更新日期：1985-03-20 00:00:00

abstract：:The multifunctional AAA-ATPase p97 is one of the most abundant and conserved proteins in eukaryotic cells. The p97/Npl4/Ufd1 complex dislocates proteins that fail the protein quality control in the endoplasmic reticulum to the cytosol where they are subject to degradation by the ubiquitin/proteasome system. Substrate ...

journal_title：Journal of molecular biology

authors： Beskow A,Grimberg KB,Bott LC,Salomons FA,Dantuma NP,Young P

更新日期：2009-12-11 00:00:00

abstract：:Methyltransferases from the m(1)A(58) tRNA methyltransferase (TrmI) family catalyze the S-adenosyl-l-methionine-dependent N(1)-methylation of tRNA adenosine 58. The crystal structure of Thermus thermophilus TrmI, in complex with S-adenosyl-l-homocysteine, was determined at 1.7 A resolution. This structure is closely r...

journal_title：Journal of molecular biology

authors： Barraud P,Golinelli-Pimpaneau B,Atmanene C,Sanglier S,Van Dorsselaer A,Droogmans L,Dardel F,Tisné C

更新日期：2008-03-21 00:00:00

abstract：:RepA protein of plasmid P1 binds to arrays of 19 bp repeat sequences (iterons) and mediates initiation of replication and its control. Escherichia coli heat shock proteins DnaJ and DnaK can stimulate iteron binding activity of RepA in an ATP-dependent fashion. It has been proposed that RepA binds to DNA as monomers an...

journal_title：Journal of molecular biology

authors： DasGupta S,Mukhopadhyay G,Papp PP,Lewis MS,Chattoraj DK

更新日期：1993-07-05 00:00:00

abstract：:RusA is a Holliday junction resolvase encoded by the cryptic prophage DLP12 of Escherichia coli K-12 that can be activated to promote homologous recombination and DNA repair in resolution-deficient mutants lacking the RuvABC proteins. Database searches with the 120 amino acid residue RusA sequence identified 11 homolo...

journal_title：Journal of molecular biology

authors： Bolt EL,Sharples GJ,Lloyd RG

更新日期：1999-02-19 00:00:00

abstract：:Tumor suppressor p53 plays a central role in tumor suppression. p53 is the most frequently mutated gene in human cancer, and over half of human cancers contain p53 mutations. Majority of p53 mutations in cancer are missense mutations, leading to the expression of full-length mutant p53 (mutp53) protein. While the crit...

journal_title：Journal of molecular biology

authors： Yue X,Zhao Y,Xu Y,Zheng M,Feng Z,Hu W

更新日期：2017-06-02 00:00:00

abstract：:Rhodanese is an ubiquitous enzyme that in vitro catalyses the transfer of a sulfur atom from suitable donors to nucleophilic acceptors by way of a double displacement mechanism. During the catalytic process the enzyme cycles between a sulfur-free and a persulfide-containing form, via formation of a persulfide linkage ...

journal_title：Journal of molecular biology

authors： Bordo D,Deriu D,Colnaghi R,Carpen A,Pagani S,Bolognesi M

更新日期：2000-05-12 00:00:00

abstract：:During vacuum condensation of metals on frozen proteins, nanoclusters are preferentially formed at specific surface sites (decoration). Understanding the nature of metal/protein interaction is of interest for structure analysis and is also important in the fields of biocompatibility and sensor development. Studies on ...

journal_title：Journal of molecular biology

authors： Braun N,Meining W,Hars U,Fischer M,Ladenstein R,Huber R,Bacher A,Weinkauf S,Bachmann L

更新日期：2002-08-09 00:00:00

abstract：:The developmental pathways for a variety of eukaryotic and prokaryotic double-stranded DNA viruses include packaging of viral DNA into a preformed procapsid structure, catalyzed by terminase enzymes and fueled by ATP hydrolysis. In most instances, a capsid expansion process accompanies DNA packaging, which significant...

journal_title：Journal of molecular biology

authors： Yang Q,Maluf NK,Catalano CE

更新日期：2008-11-28 00:00:00

abstract：:We developed a novel genetic method for finding functional regions of a protein by the analysis of chimeras formed between homologous proteins. Sets of chimeric genes were made by intramolecular homologous recombination in a linearized plasmid DNA carrying both recA genes of Escherichia coli and Pseudomonas aeruginosa...

journal_title：Journal of molecular biology

authors： Ogawa T,Shinohara A,Ogawa H,Tomizawa J

更新日期：1992-08-05 00:00:00

abstract：:Previous studies show that some yeast strains carrying point mutations of domain 5 that block splicing of a mitochondrial group II intron yield spontaneous revertants in which splicing is partially restored by dominant mutations of nuclear genes. Here we cloned and sequenced the suppressor allele of one such gene, and...

journal_title：Journal of molecular biology

authors： Schmidt U,Maue I,Lehmann K,Belcher SM,Stahl U,Perlman PS

更新日期：1998-09-25 00:00:00

abstract：:The plasma of the striped bass Morone saxatilis contains a fucose-specific lectin (MsaFBP32) that consists of two F-type carbohydrate recognition domains (CRDs) in tandem. The crystal structure of the complex of MsaFBP32 with l-fucose reported here shows a cylindrical 81-A-long and 60-A-wide trimer divided into two gl...

journal_title：Journal of molecular biology

authors： Bianchet MA,Odom EW,Vasta GR,Amzel LM

更新日期：2010-08-13 00:00:00

abstract：:The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage phi29 virus capsid. On the basis of the results of single-molecule experimental studies, we propose a push and roll mechanism to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while rotati...

journal_title：Journal of molecular biology

authors： Yu J,Moffitt J,Hetherington CL,Bustamante C,Oster G

更新日期：2010-07-09 00:00:00

abstract：:The 1.94 A resolution three-dimensional structure of oxidized horse heart cytochrome c has been elucidated and refined to a final R-factor of 0.17. This has allowed for a detailed assessment of the structural features of this protein, including the presence of secondary structure, hydrogen-bonding patterns and heme ge...

journal_title：Journal of molecular biology

authors： Bushnell GW,Louie GV,Brayer GD

更新日期：1990-07-20 00:00:00

abstract：:The dielectric constants of sickle cell hemoglobin were determined before and after gelation. The dielectric properties of oxy and deoxy sickle cell hemoglobin in solution are nearly identical to those of oxy and deoxy hemoglobin A. Only in the gel state did deoxy sickle cell hemoglobin display dielectric behavior dif...

journal_title：Journal of molecular biology

authors： Delalic Z,Takashima S,Adachi K,Asakura T

更新日期：1983-08-15 00:00:00

abstract：:Using simple design and selective pressure, we have evolved an artificial M13 bacteriophage coat protein. M13 coat proteins first reside in the bacterial inner membrane and subsequently surround the DNA core of the assembled virus. The artificial coat protein (ACP) was designed and evolved to mimic both functions of t...

journal_title：Journal of molecular biology

authors： Weiss GA,Sidhu SS

更新日期：2000-06-30 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:The trigger factor is associated with bacterial ribosomes and catalyzes proline-limited protein folding reactions. Its folding activity is very high and conserved in evolution, as shown for the homologous enzymes from Escherichia coli and Mycoplasma genitalium. The folding protein substrate (a variant of ribonuclease ...

journal_title：Journal of molecular biology

authors： Scholz C,Mücke M,Rape M,Pecht A,Pahl A,Bang H,Schmid FX

更新日期：1998-04-03 00:00:00

abstract：:Two new human cDNAs, designated phclk2 and phclk3, which have a high identity to the cDNA of the human protein kinase clk, were characterized. Typical features of hclk2 and hclk3 proteins are non-homologous N-terminal regions and the presence of the C-terminal protein kinase domain, which is characteristic for serine/...

journal_title：Journal of molecular biology

authors： Hanes J,von der Kammer H,Klaudiny J,Scheit KH

更新日期：1994-12-16 00:00:00

abstract：:The portal proteins of tailed bacteriophage and Herpesvirus capsids form dodecameric rings that occupy one capsid vertex and are incorporated during the assembly of capsid precursors called procapsids or proheads. Portals are essential and serve as the pore for DNA transit and the site of tail attachment; however, bac...

journal_title：Journal of molecular biology

authors： Maurer JB,Oh B,Moyer CL,Duda RL

更新日期：2020-03-27 00:00:00

abstract：:X-ray data have been recorded to 1.0 A resolution from a crystal of Fusarium solani cutinase using synchrotron radiation and an imaging-plate scanner. The anisotropic treatment of thermal motion led to a fivefold increase in accuracy and to a considerable quality improvement in the electron density maps with respect t...

journal_title：Journal of molecular biology

authors： Longhi S,Czjzek M,Lamzin V,Nicolas A,Cambillau C

更新日期：1997-05-16 00:00:00

abstract：:The Alu Ya-lineage is a group of related, short interspersed elements (SINEs) found in primates. This lineage includes subfamilies Ya1-Ya5, Ya5a2 and others. Some of these subfamilies are still actively mobilizing in the human genome. We have analyzed 2482 elements that reside in the human genome draft sequence and fo...

journal_title：Journal of molecular biology

authors： Otieno AC,Carter AB,Hedges DJ,Walker JA,Ray DA,Garber RK,Anders BA,Stoilova N,Laborde ME,Fowlkes JD,Huang CH,Perodeau B,Batzer MA

更新日期：2004-09-03 00:00:00

abstract：:Voltage-dependent H(+) (Hv) channels mediate proton conduction into and out of cells under the control of membrane voltage. Hv channels are unusual compared to voltage-dependent K(+), Na(+), and Ca(2+) channels in that Hv channel genes encode a voltage sensor domain (VSD) without a pore domain. The H(+) currents obser...

journal_title：Journal of molecular biology

authors： Lee SY,Letts JA,MacKinnon R

更新日期：2009-04-17 00:00:00

abstract：:A specific complex of 5 S rRNA and several ribosomal proteins is an integral part of ribosomes in all living organisms. Here we studied the importance of Escherichia coli genes rplE, rplR and rplY, encoding 5 S rRNA-binding ribosomal proteins L5, L18 and L25, respectively, for cell growth, viability and translation. U...

journal_title：Journal of molecular biology

authors： Korepanov AP,Gongadze GM,Garber MB,Court DL,Bubunenko MG

更新日期：2007-03-02 00:00:00

abstract：:The movement of the bacterial insertion sequence IS1 often generates cointegrate structures in which donor and target replicons are connected by direct repeats of IS1. The experiments reported here were designed to understand how IS1 transposition is controlled. Our physical characterization of the structures of coint...

journal_title：Journal of molecular biology

authors： Biel SW,Adelt G,Berg DE

更新日期：1984-04-05 00:00:00

abstract：:A family of Structural Maintenance of Chromosome (SMC) complexes is essential for key cellular processes ensuring proper cohesion, condensation and replication. They share a common SMC-kleisin architecture allowing them to embrace DNA. In SMC5/6, the NSE1 and NSE3 KITE and NSE4 kleisin subunits form a stable subcomple...

journal_title：Journal of molecular biology

authors： Adamus M,Lelkes E,Potesil D,Ganji SR,Kolesar P,Zabrady K,Zdrahal Z,Palecek JJ

更新日期：2020-06-12 00:00:00

abstract：:The mutants P235A and F236A have been generated and their crystal structure was determined to resolutions of 2.38 and 2.35 A, respectively, in order to understand the residues involved in the formation of the novel arched P-loop of subunit A of the A-ATP synthase from Pyrococcus horikoshii OT3. Both the structures sho...

journal_title：Journal of molecular biology

authors： Kumar A,Manimekalai MS,Balakrishna AM,Priya R,Biuković G,Jeyakanthan J,Grüber G

更新日期：2010-09-03 00:00:00