Abstract:
:The oxyR-encoded regulatory protein, OxyR, acts to induce the synthesis of a family of hydrogen peroxide-inducible proteins in Salmonella typhimurium and Escherichia coli. To further define the mechanism by which oxyR regulates the production of these proteins, we identified, mapped, and characterized oxyR-regulated promoters upstream from the S. typhimurium ahp genes (encoding an alkyl hydroperoxide reductase) and the E. coli katG gene (encoding catalase). A set of ahpC promoter deletions was constructed in vitro and analysis of these deletions revealed the location of sequences that are involved in oxyR-mediated induction of the ahpC gene product. DNase I protection studies of the ahpC promoter region revealed an oxyR-dependent footprint that overlapped the sequences found to be important for oxyR control. E. coli strains containing transcriptional fusions between the katG promoter and the lacZ gene showed strongly increased synthesis of beta-galactosidase in response to hydrogen peroxide treatment. This stimulation was found to be oxyR-dependent. DNase I protection studies of the katG promoter region revealed an oxyR-dependent footprint in the same location relative to the basal promoter elements as was observed with the ahpC promoter. Although both the ahpC and katG promoters were shown to bind the same factor, no strong sequence similarities were found between the two, or between the two and a third oxyR-dependent binding site upstream from the E. coli oxyR gene itself.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Tartaglia LA,Storz G,Ames BNdoi
10.1016/0022-2836(89)90104-6subject
Has Abstractpub_date
1989-12-20 00:00:00pages
709-19issue
4eissn
0022-2836issn
1089-8638pii
0022-2836(89)90104-6journal_volume
210pub_type
杂志文章abstract::Transcription of the rabbit alpha-globin gene begins primarily at the cap site, although some upstream start sites are also observed. Analysis by RNA polymerase run-on assays in nuclei shows that transcription continues at a high level past the polyadenylation site, after which the polymerase density actually increase...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(91)90011-t
更新日期:1991-07-20 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(88)90438-x
更新日期:1988-05-05 00:00:00
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1996.0151
更新日期:1996-03-22 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(90)90224-A
更新日期:1990-04-20 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/j.jmb.2008.02.046
更新日期:2008-06-06 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1996.0011
更新日期:1996-01-12 00:00:00
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pub_type: 杂志文章
doi:10.1006/jmbi.2000.4300
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pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1997.1580
更新日期:1998-03-27 00:00:00
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pub_type: 杂志文章
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更新日期:2003-08-22 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1986-09-05 00:00:00
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