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Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance.

Abstract：

:Two-dimensional nuclear Overhauser enhancement spectra are presented of the complex of lac repressor headpiece with a 14 base-pair lac operator fragment. Analysis of nuclear Overhauser enhancements observed between protein and DNA shows that the second helix of the headpiece ("the recognition helix") binds in the major groove of DNA as has been suggested, but that the orientation of this helix is approximately 180 degrees different from the proposed models.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Boelens R,Scheek RM,van Boom JH,Kaptein R

doi

10.1016/0022-2836(87)90638-3

subject

Has Abstract

pub_date

1987-01-05 00:00:00

pages

213-6

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90638-3

journal_volume

193

pub_type

杂志文章

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