BolA inhibits cell elongation and regulates MreB expression levels.

abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

journal_title：Journal of molecular biology

authors： Kostelidou K,Jones AC,Thomas CM

更新日期：1999-06-04 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:Several experimental data support the notion that the recognition of DNA crossovers play an important role in the multiple functions of topoisomerase II. Here, a theoretical analysis of the possible modes of assembly of yeast topoisomerase II with right and left-handed tight DNA crossovers is performed, using the crys...

journal_title：Journal of molecular biology

authors： Timsit Y,Duplantier B,Jannink G,Sikorav JL

更新日期：1998-12-18 00:00:00

abstract：:The infrared dichroic ratios of the amide bands from oriented beta-barrels yield an experimental value for the mean orientation, beta, of the beta-strands, relative to the barrel axis. For a barrel of n strands, this then gives the shear number, S, that characterizes the stagger of the beta-sheet. Combining values of ...

journal_title：Journal of molecular biology

authors： Marsh D

更新日期：2000-03-31 00:00:00

abstract：:Two major methods are currently being used to characterize transient intermediates during protein folding at the level of individual residues. Nuclear magnetic resonance (n.m.r.) measurements on the protection of peptide NH hydrogens against exchange with solvent during refolding can provide information about secondar...

journal_title：Journal of molecular biology

authors： Matouschek A,Serrano L,Meiering EM,Bycroft M,Fersht AR

更新日期：1992-04-05 00:00:00

abstract：:Riboswitches are complex folded RNA domains found in noncoding regions of mRNA that regulate gene expression upon small molecule binding. Recently, Breaker and coworkers reported a tandem aptamer riboswitch (VCI-II) that binds glycine cooperatively. Here, we use hydroxyl radical footprinting and small-angle X-ray scat...

journal_title：Journal of molecular biology

authors： Lipfert J,Das R,Chu VB,Kudaravalli M,Boyd N,Herschlag D,Doniach S

更新日期：2007-02-02 00:00:00

abstract：:MOA (Marasmius oreades agglutinin), a lectin isolated from fruiting bodies of the mushroom M. oreades, specifically binds nonreducing terminal Galalpha(1,3)Gal carbohydrates, such as that which occurs in the xenotransplantation epitope Galalpha(1,3)Galbeta(1,4)GlcNAc and the branched blood group B determinant Galalpha...

journal_title：Journal of molecular biology

authors： Grahn EM,Winter HC,Tateno H,Goldstein IJ,Krengel U

更新日期：2009-07-17 00:00:00

abstract：:The structures of the two very closely related proteins, bovine gamma II- and gamma IVa-crystallin have been studied by means of near-ultra-violet linear dichroism spectroscopy on squeezed polyacrylamide gel systems. The crystallin spectra are discussed in terms of the spectra of the aromatic chromophores present in t...

journal_title：Journal of molecular biology

authors： Bloemendal M,Leunissen JA,van Amerongen H,van Grondelle R

更新日期：1990-11-05 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Herpes simplex viruses (HSVs) are human pathogens that establish lytic and latent infections. Reactivation from latency occurs intermittently, which represents a lifelong source of recurrent infection. In this complex process, HSV triggers and neutralizes innate immunity. Therefore, a dynamic equilibrium between HSV a...

journal_title：Journal of molecular biology

authors： Ma Y,He B

更新日期：2014-03-20 00:00:00

abstract：:Huntington's disease (HD) is an inherited progressive neurodegenerative disorder caused by a pathological CAG trinucleotide repeat expansion in the large multi-exon gene, huntingtin (HTT). Although multiple pathogenic mechanisms have been proposed for HD, there is increasing interest in the RNA processing of the HTT g...

journal_title：Journal of molecular biology

authors： Hughes AC,Mort M,Elliston L,Thomas RM,Brooks SP,Dunnett SB,Jones L

更新日期：2014-04-03 00:00:00

abstract：:Bacteriophage T4 provides an important model for the biochemistry and genetics of DNA metabolism. Phage-encoded proteins conduct all essential steps of T4 DNA replication, repair, and recombination. Central to these three processes is the T4 UvsX protein, a member of the filamentous, ATP-dependent class of general rec...

journal_title：Journal of molecular biology

authors： Ando RA,Morrical SW

更新日期：1998-11-06 00:00:00

abstract：:The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an excised Ssp DnaE intein and the second from a splicing-deficient precu...

journal_title：Journal of molecular biology

authors： Sun P,Ye S,Ferrandon S,Evans TC,Xu MQ,Rao Z

更新日期：2005-11-11 00:00:00

abstract：:Using computational and sequence analysis of bacterial cold shock proteins, we designed a protein (CspB-TB) that has the core residues of mesophilic protein from Bacillus subtilis(CspB-Bs) and altered distribution of surface charged residues. This designed protein was characterized by circular dichroism spectroscopy, ...

journal_title：Journal of molecular biology

authors： Makhatadze GI,Loladze VV,Gribenko AV,Lopez MM

更新日期：2004-02-27 00:00:00

abstract：:We have previously described the organization of a 13.3 kb region of the Aspergillus nidulans genome, designated SpoC1, coding for multiple poly(A)+ RNAs that accumulate in asexual spores but not in somatic cells. We have determined the limits of the SpoC1 gene cluster by investigating the transcriptional features of ...

journal_title：Journal of molecular biology

authors： Gwynne DI,Miller BL,Miller KY,Timberlake WE

更新日期：1984-11-25 00:00:00

abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

journal_title：Journal of molecular biology

authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

更新日期：2007-12-07 00:00:00

abstract：:Biochemical functions of proteins in cells frequently involve interactions with various ligands. Proteomic methods for the identification of proteins that interact with specific ligands such as metabolites, signaling molecules, and drugs are valuable in investigating the regulatory mechanisms of cellular metabolism, a...

journal_title：Journal of molecular biology

authors： Liu PF,Kihara D,Park C

更新日期：2011-04-22 00:00:00

abstract：:The U6 RNA internal stem-loop (U6 ISL) is a highly conserved domain of the spliceosome that is important for pre-mRNA splicing. The U6 ISL contains an internal loop that is in equilibrium between two conformations controlled by the protonation state of an adenine (pK(a)=6.5). Lower pH favors formation of a protonated ...

journal_title：Journal of molecular biology

authors： Venditti V,Clos L 2nd,Niccolai N,Butcher SE

更新日期：2009-09-04 00:00:00

abstract：:Islet amyloid polypeptide (IAPP) forms fibrillar amyloid deposits in the pancreatic islets of Langerhans of patients with type 2 diabetes mellitus, and its misfolding and aggregation are thought to contribute to beta-cell death. Increasing evidence suggests that IAPP fibrillization is strongly influenced by lipid memb...

journal_title：Journal of molecular biology

authors： Domanov YA,Kinnunen PK

更新日期：2008-02-08 00:00:00

abstract：:Nebulin, a large protein (600 to 800 kDa) located in the thin filament of striated vertebrate muscle, is assumed to bind and stabilise F-actin. Complete sequence determination of human nebulin has only recently been accomplished showing a uniform modular structure along the whole length of the molecule. Up to 97% of t...

journal_title：Journal of molecular biology

authors： Pfuhl M,Winder SJ,Castiglione Morelli MA,Labeit S,Pastore A

更新日期：1996-03-29 00:00:00

abstract：:The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to mo...

journal_title：Journal of molecular biology

authors： Bujalowski W,Lohman TM

更新日期：1991-01-05 00:00:00

abstract：:Human serum amyloid P component (SAP) is a normal plasma glycoprotein and the precursor of amyloid P component which is a universal constituent of the abnormal tissue deposits in amyloidosis. X-ray and neutron scattering data showed that pentameric or decameric ring structures for SAP in solution are readily distingui...

journal_title：Journal of molecular biology

authors： Ashton AW,Boehm MK,Gallimore JR,Pepys MB,Perkins SJ

更新日期：1997-09-26 00:00:00

abstract：:Expression of the Caenorhabditis elegans cysteine protease gene cpr-1 is regulated both spatially and temporally. In situ hybridisation and Northern blot analysis have shown that this gene is expressed exclusively in gut cells of all developmental stages except the embryo. We now show by transgenic transformation with...

journal_title：Journal of molecular biology

authors： Britton C,McKerrow JH,Johnstone IL

更新日期：1998-01-01 00:00:00

abstract：:Although the quaternary state has been assessed in detail for only a few members of the major facilitator superfamily (MFS), it is clear that multiple oligomeric states are represented within the MFS. One of its members, the lactose transporter LacS from Streptococcus thermophilus assumes a dimeric structure in the me...

journal_title：Journal of molecular biology

authors： Geertsma ER,Duurkens RH,Poolman B

更新日期：2005-07-01 00:00:00

abstract：:Small multidrug resistance (SMR) transporters contribute to bacterial resistance by coupling the efflux of a wide range of toxic aromatic cations, some of which are commonly used as antibiotics and antiseptics, to proton influx. EmrE is a prototypical small multidrug resistance transporter comprising four transmembran...

journal_title：Journal of molecular biology

authors： Fleishman SJ,Harrington SE,Enosh A,Halperin D,Tate CG,Ben-Tal N

更新日期：2006-11-17 00:00:00

abstract：:The folding kinetics and thermodynamics of the isolated C-terminal domain of the ribosomal protein L9 (CTL9) have been studied as a function of pH. CTL9 is an alpha-beta protein that contains a single beta-sheet with an unusual mixed parallel, anti-parallel topology. The folding is fully reversible and two-state over ...

journal_title：Journal of molecular biology

authors： Sato S,Raleigh DP

更新日期：2002-04-26 00:00:00

abstract：:In this paper we describe a two-plasmid system which allows over-production of the R.EcoR124I restriction endonuclease. The endonuclease has been purified to homogeneity in milligram amounts and has been shown to be fully active for both restriction and modification. Unexpectedly, the enzyme was found to require only ...

journal_title：Journal of molecular biology

authors： Janscak P,Abadjieva A,Firman K

更新日期：1996-04-19 00:00:00

abstract：:Many proteins are composed of independently-folded domains connected by flexible linkers. The primary sequence and length of such linkers can set the effective concentration for the tethered domains, which impacts rates of association and enzyme activity. The length of such linkers can be sensitive to environmental co...

journal_title：Journal of molecular biology

authors： Basak S,Sakia N,Dougherty L,Guo Z,Wu F,Mindlin F,Lary JW,Cole JL,Ding F,Bowen ME

更新日期：2021-01-01 00:00:00

abstract：:Efficient gene control by antisense RNA requires rapid bi-molecular interaction with a cognate target RNA. A comparative analysis revealed that a YUNR motif (Y=pyrimidine, R=purine) is ubiquitous in RNA recognition loops in antisense RNA-regulated gene systems. The (Y)UNR sequence motif specifies two intraloop hydroge...

journal_title：Journal of molecular biology

authors： Franch T,Petersen M,Wagner EG,Jacobsen JP,Gerdes K

更新日期：1999-12-17 00:00:00

abstract：:alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persist...

journal_title：Journal of molecular biology

authors： Schulman BA,Redfield C,Peng ZY,Dobson CM,Kim PS

更新日期：1995-11-10 00:00:00