Abstract:
:To investigate the relationships between protein topology, amino acid sequence and folding mechanisms, the folding transition state of the Sso7d protein has been characterised both experimentally and theoretically. Although Sso7d protein has a similar topology to that of the SH3 domains, the structure of its transition state is different from that of alpha-spectrin and src SH3 domains previously studied. The folding algorithm, Fold-X, including an energy function with specific sequence features, accounts for these differences and reproduces with a good agreement the set of experimental phi(double dagger-U) values obtained for the three proteins. Our analysis shows that taking into account sequence features underlying protein topology is critical for an accurate prediction of the folding process.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Guerois R,Serrano Ldoi
10.1006/jmbi.2000.4234keywords:
subject
Has Abstractpub_date
2000-12-15 00:00:00pages
967-82issue
5eissn
0022-2836issn
1089-8638pii
S0022-2836(00)94234-7journal_volume
304pub_type
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