Abstract:
:The 69 nucleotide left-terminal domain (T(L)) of the potato spindle tuber RNA viroid (PSTVd) constitutes one of its five structural elements. Due to a twofold complementary sequence repeat, two possible conformations are proposed for the T(L) secondary structure; an elongated-rod and a bifurcated form. In the present study, two T(L) mutants were designed that remove the symmetry of the sequence repeats and ensure that either the bifurcated or the elongated-rod conformation is thermodynamically favored. Imino 1H and 15N resonances were assigned for both mutants and the native T(L) domain based on 1H-1H NOESY and heteronuclear 1H-15N HSQC high-resolution NMR spectra. The NMR secondary structure analysis of all constructs establishes unambiguously the elongated-rod form as the secondary structure of the native T(L) domain. Temperature-gradient gel electrophoresis and UV melting experiments corroborate these results. A combined secondary structure and sequence analysis of T(L) domains of other Pospiviroidae family members indicates that the elongated-rod form is thermodynamically favored for the vast majority of these viroids.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Dingley AJ,Steger G,Esters B,Riesner D,Grzesiek Sdoi
10.1016/j.jmb.2003.10.015keywords:
subject
Has Abstractpub_date
2003-12-05 00:00:00pages
751-67issue
4eissn
0022-2836issn
1089-8638pii
S0022283603012646journal_volume
334pub_type
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