Lambda phage protein Nu 1 contains the conserved DNA binding fold of repressors.

abstract：:The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectrosco...

journal_title：Journal of molecular biology

authors： Mao H,Williamson JR

更新日期：1999-09-17 00:00:00

abstract：:The intermembrane space of mitochondria contains a dedicated chaperone network-the small translocase of the inner membrane (TIM) family-for the sorting of hydrophobic precursors. All small TIMs are defined by the presence of a twin CX(3)C motif and the monomeric proteins are stabilized by two intramolecular disulfide ...

journal_title：Journal of molecular biology

authors： Baker MJ,Mooga VP,Guiard B,Langer T,Ryan MT,Stojanovski D

更新日期：2012-12-14 00:00:00

abstract：:This study describes an algorithm that finds rho-independent transcription terminators in bacterial genomes and evaluates the accuracy of its predictions. The algorithm identifies terminators by searching for a common mRNA motif: a hairpin structure followed by a short uracil-rich region. For each terminator, an energ...

journal_title：Journal of molecular biology

authors： Ermolaeva MD,Khalak HG,White O,Smith HO,Salzberg SL

更新日期：2000-08-04 00:00:00

abstract：:As the receptor on the platelet surface for von Willebrand factor, glycoprotein (GP) Ib-IX complex is critically involved in hemostasis and thrombosis. How the complex is assembled from GP Ibalpha, GP Ib beta and GP IX subunits, all of which are type I transmembrane proteins, is not entirely clear. Genetic and mutatio...

journal_title：Journal of molecular biology

authors： Luo SZ,Li R

更新日期：2008-10-03 00:00:00

abstract：:Expansion of the polyglutamine (polyQ) region in the protein ataxin-3 is associated with spinocerebellar ataxia type 3, an inherited neurodegenerative disorder that belongs to the family of polyQ diseases. Increasing evidence indicates that protein aggregation and fibre formation play an important role in these pathol...

journal_title：Journal of molecular biology

authors： Masino L,Nicastro G,Menon RP,Dal Piaz F,Calder L,Pastore A

更新日期：2004-12-03 00:00:00

abstract：:Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of th...

journal_title：Journal of molecular biology

authors： Atzenhofer W,Regelsberger G,Jacob U,Peschek G,Furtmüller P,Huber R,Obinger C

更新日期：2002-08-16 00:00:00

abstract：:The FLP recombinase of the 2 mu plasmid of Saccharomyces cerevisiae binds to a target containing three 13 base-pair symmetry elements called a, b and c. The symmetry elements b and c are in direct orientation while the a element is in inverted orientation with respect to b and c on the opposite side of an eight base-p...

journal_title：Journal of molecular biology

authors： Schwartz CJ,Sadowski PD

更新日期：1990-11-20 00:00:00

abstract：:Light chain amyloidosis is a devastating disease where immunoglobulin light chains form amyloid fibrils, resulting in organ dysfunction and death. Previous studies have shown a direct correlation between the protein thermodynamic stability and the propensity for amyloid formation for some proteins involved in light ch...

journal_title：Journal of molecular biology

authors： Blancas-Mejía LM,Tischer A,Thompson JR,Tai J,Wang L,Auton M,Ramirez-Alvarado M

更新日期：2014-01-23 00:00:00

abstract：:Functional large ribosomal subunits of Thermus aquaticus can be reconstituted from ribosomal proteins and either natural or in vitro transcribed 23 S and 5 S rRNA. Omission of 5 S rRNA during subunit reconstitution results in dramatic decrease of the peptidyl transferase activity of the assembled subunits. However, th...

journal_title：Journal of molecular biology

authors： Khaitovich P,Mankin AS

更新日期：1999-09-03 00:00:00

abstract：:The secondary structure of the Escherichia coli alpha mRNA leader sequence has been determined using nucleases specific for single- or double-stranded RNA. Three different length alpha RNA fragments were studied at 0 degrees C and 37 degrees C. A very stable eight base-pair helix forms upstream from the ribosome initi...

journal_title：Journal of molecular biology

authors： Deckman IC,Draper DE

更新日期：1987-07-20 00:00:00

abstract：:The type A domain of the von Willebrand Factor is found also in the complement proteins factor B (FB), C2, CR3 and CR4, the integrins, collagen types VI, VII, XII and XIV, and other proteins. FB is a component of the alternative pathway of the complement system of immune defence, and is cleaved into the fragments Bb a...

journal_title：Journal of molecular biology

authors： Perkins SJ,Smith KF,Williams SC,Haris PI,Chapman D,Sim RB

更新日期：1994-04-22 00:00:00

abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

journal_title：Journal of molecular biology

authors： Vogelaar NJ,Lindberg U,Schutt CE

更新日期：1991-08-05 00:00:00

abstract：:New methods for collecting cross-relaxation data from proteins and nucleic acids make it possible to improve the accuracy and precision of interproton distance measurements used as input for NMR solution structure determinations. It thus is of interest to determine whether such experimentally achievable improvements i...

journal_title：Journal of molecular biology

authors： Hoogstraten CG,Markley JL

更新日期：1996-05-03 00:00:00

abstract：:RNA splicing brings diversity to the eukaryotic proteome. Different spliced variants of a gene may differ in their structure, function, localization, and stability influencing protein stoichiometry and physiological outcomes. Alternate spliced variants of different genes are known to associate with various chronic pat...

journal_title：Journal of molecular biology

authors： Chauhan K,Kalam H,Dutt R,Kumar D

更新日期：2019-04-05 00:00:00

abstract：:We have carried out molecular dynamics simulations to study the conformational equilibria of two blocked dipeptides, Ac-Ala-Ala-NHMe and trans-Ac-Pro-Ala-NHMe, in water (Ac, amino-terminal blocking group COCH3; NHMe, carboxy-terminal blocking group NHCH3). Using specialized sampling techniques we computed free-energy ...

journal_title：Journal of molecular biology

authors： Tobias DJ,Sneddon SF,Brooks CL 3rd

更新日期：1990-12-05 00:00:00

abstract：:The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled w...

journal_title：Journal of molecular biology

authors： Luo Y,Wu JL,Li B,Langsetmo K,Gergely J,Tao T

更新日期：2000-02-25 00:00:00

abstract：:The temperature induced unfolding/dissociation of the dimeric subtilisin inhibitor from Streptomyces and its mutant D83C having an S-S crosslink between the subunits has been studied calorimetrically. Comparison of the entropies measured at different concentrations of dimer showed that the entropy cost of crosslinking...

journal_title：Journal of molecular biology

authors： Tamura A,Privalov PL

更新日期：1997-11-14 00:00:00

abstract：:Little is known concerning promoter structure in the filarial parasites. Recently, transient transfection methods have been developed for the human filarial parasite Brugia malayi. These methods have been employed to localize the promoter for the 70kDa heat shock protein (BmHSP70) to a region extending 394nt upstream ...

journal_title：Journal of molecular biology

authors： Higazi TB,Deoliveira A,Katholi CR,Shu L,Barchue J,Lisanby M,Unnasch TR

更新日期：2005-10-14 00:00:00

abstract：:The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage phi29 virus capsid. On the basis of the results of single-molecule experimental studies, we propose a push and roll mechanism to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while rotati...

journal_title：Journal of molecular biology

authors： Yu J,Moffitt J,Hetherington CL,Bustamante C,Oster G

更新日期：2010-07-09 00:00:00

abstract：:The T7 RNA polymerase promoter has been proposed to contain two domains: the binding region upstream of position -5 is recognized through apparently traditional duplex contacts, while the catalytic domain downstream of position -5 is bound in a melted configuration. This model is tested by following polymerase binding...

journal_title：Journal of molecular biology

authors： Ujvári A,Martin CT

更新日期：1997-11-07 00:00:00

abstract：:The repertoire of naturally occurring protein structures is usually characterised in structural terms at the domain level by their constituent folds. As structure is acknowledged to be an important stepping stone to the understanding of protein function, an appreciation of how individual domain interactions are built ...

journal_title：Journal of molecular biology

authors： Littler SJ,Hubbard SJ

更新日期：2005-02-04 00:00:00

abstract：:The conformation of NAD+ in the sheep liver sorbitol dehydrogenase-NAD+ binary complex has been investigated using time-dependent proton-proton transferred nuclear Overhauser enhancement measurements to determine interproton distance ratios and distances between bound NAD+ protons. The conformation about both the aden...

journal_title：Journal of molecular biology

authors： Gronenborn AM,Clore GM,Jeffery J

更新日期：1984-02-05 00:00:00

abstract：:In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. The autoinducer-2 production protein LuxS, is involved in a novel quorum-sensing system and is thought to catalyse the degradation of S-ribosylhomocysteine to homocysteine and the autoinducer molecule 4,5-di...

journal_title：Journal of molecular biology

authors： Ruzheinikov SN,Das SK,Sedelnikova SE,Hartley A,Foster SJ,Horsburgh MJ,Cox AG,McCleod CW,Mekhalfia A,Blackburn GM,Rice DW,Baker PJ

更新日期：2001-10-12 00:00:00

abstract：:The disulphide active sites of thioredoxin and DsbA are known to possess a high degree of structural homology. However, DsbA is a much stronger oxidant than thioredoxin. The redox potential difference between DsbA and thioredoxin has been measured to be 160 mV, equivalent to a shift of 15.4 kJ/mol in the reduced/oxidi...

journal_title：Journal of molecular biology

authors： Gane PJ,Freedman RB,Warwicker J

更新日期：1995-06-02 00:00:00

abstract：:Homodimeric bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta (TGF-beta) superfamily that induces bone formation and regeneration, and determines important steps during early stages of embryonic development in vertebrates and non-vertebrates. BMP-2 can interact with two types of r...

journal_title：Journal of molecular biology

authors： Scheufler C,Sebald W,Hülsmeyer M

更新日期：1999-03-19 00:00:00

abstract：:Methods that compare a protein sequence directly to a structure can be divided into those that construct a molecular model (threading methods) and those that perform a sequence alignment with the structure encoded as a sequence of structural states (one-dimensional/three-dimensional (1D/3D) matching). The former take ...

journal_title：Journal of molecular biology

authors： Taylor WR

更新日期：1997-06-27 00:00:00

abstract：:Retinoic-acid mediated differentiation of F9 cells is accompanied by an increased transcription of the histone H1zero gene. This increase is an early response after addition of retinoic acid, suggesting a direct effect of the hormone on transcription of the gene. We show now that the promoter of histone H1zero contain...

journal_title：Journal of molecular biology

authors： Mader S,Thiele K,Breuer B,Alonso A

更新日期：1994-09-09 00:00:00

abstract：:14-3-3 proteins have been shown to regulate the actin cytoskeleton remodeling, cell adhesion and migration. In this study, we identified ezrin, a cross-linker between plasma membrane and actin cytoskeleton, as a novel 14-3-3ζ interacting partner. The direct interaction between 14-3-3ζ and ezrin was validated in the ce...

journal_title：Journal of molecular biology

authors： Chen M,Liu T,Xu L,Gao X,Liu X,Wang C,He Q,Zhang G,Liu L

更新日期：2014-09-09 00:00:00

abstract：:AMP phosphorylase (AMPpase) catalyzes the initial reaction in a novel AMP metabolic pathway recently found in archaea, converting AMP and phosphate into adenine and ribose 1,5-bisphosphate. Gel-filtration chromatography revealed that AMPpase from Thermococcus kodakarensis (Tk-AMPpase) forms an exceptionally large macr...

journal_title：Journal of molecular biology

authors： Nishitani Y,Aono R,Nakamura A,Sato T,Atomi H,Imanaka T,Miki K

更新日期：2013-08-09 00:00:00

abstract：:We have been able to observe the first step in bacteriophage infection, the docking of phage lambda to its membrane receptor maltoporin, at the single-particle level. High-resolution conductance recording from a single trimeric maltoporin channel reconstituted into a planar lipid bilayer has allowed detection of the s...

journal_title：Journal of molecular biology

authors： Gurnev PA,Oppenheim AB,Winterhalter M,Bezrukov SM

更新日期：2006-06-23 00:00:00