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Design and crystal structure of bacteriophage T4 mini-fibritin NCCF.

Abstract：

:Fibritin is a fibrous protein that forms "whiskers" attached to the neck of bacteriophage T4. Whiskers interact with the long tail fibers regulating the assembly and infectivity of the virus. The fibritin trimer includes the N-terminal domain responsible for attachment to the phage particle and for the collar formation, the central domain forming a 500 A long segmented coiled-coil structure, and the C-terminal "foldon" domain. We have designed a "mini" fibritin with most of the coiled-coil domain deleted, and solved its crystal structure. The non-helical N-terminal part represents a new protein fold that tightly interacts with the coiled-coil segment forming a single domain, as revealed by calorimetry. The analysis of the crystal structure and earlier electron microscopy data on the collar-whisker complex suggests the necessity of other proteins to participate in the collar formation. Crystal structure determination of the N-terminal domain of fibritin is the first step towards elucidating the detailed structure and assembly mechanism of the collar-whisker complex.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Boudko SP,Strelkov SV,Engel J,Stetefeld J

doi

10.1016/j.jmb.2004.04.001

keywords:

subject

Has Abstract

pub_date

2004-06-11 00:00:00

pages

927-35

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022283604004048

journal_volume

339

pub_type

杂志文章

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