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Structural studies of cytochrome reductase. Improved membrane crystals of the enzyme complex and crystallization of a subcomplex.

Abstract：

:Membrane crystals of mitochondrial ubiquinol: cytochrome c reductase of improved size and long-range order and of the cytochrome bc1 subcomplex have been obtained by a dialysis method. The enzyme--Triton X-100 complex was mixed with Triton phospholipid micelles and the Triton slowly removed by dialysis for 48 hours at pH 5.5 at room temperature or above. The effect of varying the pH and temperature on the shape, size and order of the crystals is described.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Hovmöller S,Slaughter M,Berriman J,Karlsson B,Weiss H,Leonard K

doi

10.1016/s0022-2836(83)80264-2

subject

Has Abstract

pub_date

1983-04-05 00:00:00

pages

401-6

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)80264-2

journal_volume

165

pub_type

杂志文章

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