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Undesigned selection for replication termination of bacterial chromosomes.

Abstract：

:The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kono N,Arakawa K,Sato M,Yoshikawa H,Tomita M,Itaya M

doi

10.1016/j.jmb.2014.06.005

subject

Has Abstract

pub_date

2014-08-12 00:00:00

pages

2918-27

issue

16

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(14)00285-X

journal_volume

426

pub_type

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