Abstract:
:Cutinase belongs to a group of enzymes that catalyze the hydrolysis of esters and triglycerides. Structural studies on the enzyme from Fusarium solani have revealed the presence of a classic catalytic triad that has been implicated in the enzyme's mechanism. We have solved the crystal structure of Glomerella cingulata cutinase in the absence and in the presence of the inhibitors E600 (diethyl p-nitrophenyl phosphate) and PETFP (3-phenethylthio-1,1,1-trifluoropropan-2-one) to resolutions between 2.6 and 1.9 A. Analysis of these structures reveals that the catalytic triad (Ser136, Asp191, and His204) adopts an unusual configuration with the putative essential histidine His204 swung out of the active site into a position where it is unable to participate in catalysis, with the imidazole ring 11 A away from its expected position. Solution-state NMR experiments are consistent with the disrupted configuration of the triad observed crystallographically. H204N, a site-directed mutant, was shown to be catalytically inactive, confirming the importance of this residue in the enzyme mechanism. These findings suggest that, during its catalytic cycle, cutinase undergoes a significant conformational rearrangement converting the loop bearing the histidine from an inactive conformation, in which the histidine of the triad is solvent exposed, to an active conformation, in which the triad assumes a classic configuration.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Nyon MP,Rice DW,Berrisford JM,Hounslow AM,Moir AJ,Huang H,Nathan S,Mahadi NM,Bakar FD,Craven CJdoi
10.1016/j.jmb.2008.10.050subject
Has Abstractpub_date
2009-01-09 00:00:00pages
226-35issue
1eissn
0022-2836issn
1089-8638pii
S0022-2836(08)01341-7journal_volume
385pub_type
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