Influences of separation and adjacent sequences on the use of alternative 5' splice sites.

abstract：:In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. The autoinducer-2 production protein LuxS, is involved in a novel quorum-sensing system and is thought to catalyse the degradation of S-ribosylhomocysteine to homocysteine and the autoinducer molecule 4,5-di...

journal_title：Journal of molecular biology

authors： Ruzheinikov SN,Das SK,Sedelnikova SE,Hartley A,Foster SJ,Horsburgh MJ,Cox AG,McCleod CW,Mekhalfia A,Blackburn GM,Rice DW,Baker PJ

更新日期：2001-10-12 00:00:00

abstract：:Catalase-1, one of four catalase activities of Neurospora crassa, is associated with non-growing cells and accumulates in asexual spores. It is a large, tetrameric, highly efficient, and durable enzyme that is active even at molar concentrations of hydrogen peroxide. Catalase-1 is oxidized at the heme by singlet oxyge...

journal_title：Journal of molecular biology

authors： Díaz A,Horjales E,Rudiño-Piñera E,Arreola R,Hansberg W

更新日期：2004-09-17 00:00:00

abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

journal_title：Journal of molecular biology

authors： Herzberg O

更新日期：1991-02-20 00:00:00

abstract：:In vivo, proteins occur in widely different physio-chemical environments, and, from in vitro studies, we know that protein structure can be very sensitive to environment. However, theoretical studies of protein structure have tended to ignore this complexity. In this paper, we have approached this problem by grouping ...

journal_title：Journal of molecular biology

authors： Andrade MA,O'Donoghue SI,Rost B

更新日期：1998-02-20 00:00:00

abstract：:Prions are composed largely, if not entirely, of the scrapie isoform of the prion protein (PrPSc). Conversion of the cellular isoform (PrPC) to PrPSc is accompanied by a diminution in the alpha-helical content and an increase in the beta-sheet structure. To investigate the structural basis of this transition, peptide ...

journal_title：Journal of molecular biology

authors： Zhang H,Kaneko K,Nguyen JT,Livshits TL,Baldwin MA,Cohen FE,James TL,Prusiner SB

更新日期：1995-07-21 00:00:00

abstract：:Nucleocapsid assembly is an essential process in the replication of the non-segmented, negative-sense RNA viruses (NNVs). Unassembled nucleoprotein (N(0)) is maintained in an RNA-free and monomeric form by its viral chaperone, the phosphoprotein (P), forming the N(0)-P complex. Our earlier work solved the structure of...

journal_title：Journal of molecular biology

authors： Yabukarski F,Leyrat C,Martinez N,Communie G,Ivanov I,Ribeiro EA Jr,Buisson M,Gerard FC,Bourhis JM,Jensen MR,Bernadó P,Blackledge M,Jamin M

更新日期：2016-07-03 00:00:00

abstract：:Pseudomonas putida uses L-phenylalanine as the sole nitrogen source for growth by converting L-phenylalanine to L-tyrosine, which acts as a donor of the amino group. This metabolic step requires the products of the phhA and phhB genes, which form an operon. Expression of the phhA promoter is mediated by the phhR gene ...

journal_title：Journal of molecular biology

authors： Herrera MC,Ramos JL

更新日期：2007-03-09 00:00:00

abstract：:The X-ray crystal structure of a catalytic-site mutant EQ208 [Glu208-->Gln] of alpha-amylase from Bacillus subtilis cocrystallized with maltopentaose (G5) and acarbose has been determined by multiple isomorphous replacement at 2.5 A resolution. Restrained crystallographic refinement has resulted in an R-factor of 19.8...

journal_title：Journal of molecular biology

authors： Fujimoto Z,Takase K,Doui N,Momma M,Matsumoto T,Mizuno H

更新日期：1998-03-27 00:00:00

abstract：:The folding pathway of Rd-apocytochrome b562, a four-helix bundle protein, was characterized using Trp and Ala/Gly pair mutations. We found that the Trp mutants (F65W) of both the fully folded Rd-apocytochrome b562 and a partially unfolded intermediate with the N-terminal helix (helix I) unfolded, fold with identical ...

journal_title：Journal of molecular biology

authors： Zhou Z,Huang Y,Bai Y

更新日期：2005-09-30 00:00:00

abstract：:Bovine leukemia virus (BLV) is a deltaretrovirus that infects domestic cattle. The structural protein Gag, found in all retroviruses, is a polyprotein comprising three major functional domains: matrix (MA), capsid (CA), and nucleocapsid (NC). Previous studies have shown that both mature BLV MA and NC are able to bind ...

journal_title：Journal of molecular biology

authors： Qualley DF,Cooper SE,Ross JL,Olson ED,Cantara WA,Musier-Forsyth K

更新日期：2019-03-15 00:00:00

abstract：:Peptide deformylases (PDFs) are essential enzymes of the N-terminal protein processing pathway of eubacteria. The recent discovery of two types of PDFs in higher plants, PDF1A and PDF1B, and the detection of PDF1A in humans, have raised questions concerning the importance of deformylation in eukaryotes. Here, we have ...

journal_title：Journal of molecular biology

authors： Serero A,Giglione C,Meinnel T

更新日期：2001-12-07 00:00:00

abstract：:ETV6 (or TEL), a transcriptional repressor belonging to the ETS family, is frequently involved in chromosomal translocations linked with human cancers. It displays a DNA-binding mode distinct from other ETS proteins due to the presence of a self-associating PNT domain. In this study, we used NMR spectroscopy to dissec...

journal_title：Journal of molecular biology

authors： Coyne HJ 3rd,De S,Okon M,Green SM,Bhachech N,Graves BJ,McIntosh LP

更新日期：2012-08-03 00:00:00

abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

journal_title：Journal of molecular biology

authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

更新日期：2000-05-05 00:00:00

abstract：:The put sites of phage HK022 increase the processivity of transcription and thereby promote the expression of viral genes that are located downstream of transcription terminators. RNA polymerase molecules that have traversed a put site are converted to a terminator-resistant form by the put transcript. We analyzed the...

journal_title：Journal of molecular biology

authors： Banik-Maiti S,King RA,Weisberg RA

更新日期：1997-10-10 00:00:00

abstract：:The contribution of the tRNA "core" to aminoacylation is beginning to be recognized. One example is the core region of Escherichia coli tRNA(Cys), which has been shown by biochemical studies to be important for aminoacylation. This core has several layers of unusual base-pairs, which are revealed by the recent crystal...

journal_title：Journal of molecular biology

authors： Hamann CS,Hou YM

更新日期：2000-01-28 00:00:00

abstract：:The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes. CYP2R1 catalyzes the initial step converting vitamin D into 25-hydroxyvitamin D. A CYP2R1 gene mutation causes an inherited form of rickets due to 25-hydroxylase deficiency. To understand the narrow substrate specificity of CYP2R1...

journal_title：Journal of molecular biology

authors： Strushkevich N,Usanov SA,Plotnikov AN,Jones G,Park HW

更新日期：2008-06-27 00:00:00

abstract：:The Escherichia coli cyclic AMP receptor protein, CRP, induces transcription at Class II CRP-dependent promoters by making three different activatory contacts with different surfaces of holo RNA polymerase. One contact surface of CRP, known as Activating Region 3 (AR3), is functional in the downstream subunit of the C...

journal_title：Journal of molecular biology

authors： Rhodius VA,Busby SJ

更新日期：2000-06-02 00:00:00

abstract：:We have mapped the origin of DNA replication (oriB) downstream of the 23 S rRNA gene in each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 and 131,924 (IR(A)) by a combination of approaches. In vivo chloroplast DNA replication intermediates were examined by two-dimensional agaro...

journal_title：Journal of molecular biology

authors： Kunnimalaiyaan M,Shi F,Nielsen BL

更新日期：1997-05-02 00:00:00

abstract：:Anthracycline antibiotics daunomycin and adriamycin are among the most widely used in cancer chemotherapy and DNA is believed to be the primary target of their biological action. The crystal structure of a morpholino derivative of adriamycin bound to the DNA hexamer d(CGTACG) has been determined at 1.5 A resolution. T...

journal_title：Journal of molecular biology

authors： Cirilli M,Bachechi F,Ughetto G,Colonna FP,Capobianco ML

更新日期：1993-04-05 00:00:00

abstract：:The structure of the recombinant medium chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been solved by the multiple anomalous dispersion technique using the signal from the naturally occurring zinc ions. The enzyme is a tetramer with 222 point group symmetry. The ADH monomer ...

journal_title：Journal of molecular biology

authors： Guy JE,Isupov MN,Littlechild JA

更新日期：2003-08-29 00:00:00

abstract：:Phenylalanyl-tRNA synthetase (EC 6.1.1.20) from the extreme thermophile Thermus thermophilus HB8 has been isolated and crystallized. The enzyme was found to consist of two types of subunits with molecular masses 38 X 10(3) (alpha) and 94 X 10(3) (beta) and is likely to be a tetrameric protein with a molecular mass of ...

journal_title：Journal of molecular biology

authors： Chernaya MM,Korolev SV,Reshetnikova LS,Safro MG

更新日期：1987-12-05 00:00:00

abstract：:The aim of the present study was to develop the protein fragment complementation assay (PCA) for the intracellular selection of specific binding molecules from the fully synthetic HuCAL antibody library. Here, we describe the first successful selections of specific antibodies by PCA, and we discuss the opportunities a...

journal_title：Journal of molecular biology

authors： Koch H,Gräfe N,Schiess R,Plückthun A

更新日期：2006-03-24 00:00:00

abstract：:Interactions of transmembrane helices play a crucial role in the folding and oligomerisation of integral membrane proteins. In order to uncover novel sequence motifs mediating these interactions, we randomised one face of a transmembrane helix with a set of non-polar or moderately polar amino acids. Those sequences ca...

journal_title：Journal of molecular biology

authors： Unterreitmeier S,Fuchs A,Schäffler T,Heym RG,Frishman D,Langosch D

更新日期：2007-11-30 00:00:00

abstract：:The structure of three consecutive laminin-type EGF-like (LE) modules of mouse laminin gammma1 chain, gamma1III3-5 (positions 738 to 899), has been determined by multiple isomorphous replacement in a crystal of space group p6(4)22 (a=b=74.57 angstroms, c = 185.11 angstroms and gamma = 120 degrees). The crystal structu...

journal_title：Journal of molecular biology

authors： Stetefeld J,Mayer U,Timpl R,Huber R

更新日期：1996-04-05 00:00:00

abstract：:It is becoming increasingly clear that the intrinsic and protein-induced topological properties of the DNA helix influence transcriptional efficiency. In this report we describe the properties of two upstream activating regions that influence transcription from the non-overlapping tandem promoters of the ilvGMEDA oper...

journal_title：Journal of molecular biology

authors： Pagel JM,Winkelman JW,Adams CW,Hatfield GW

更新日期：1992-04-20 00:00:00

abstract：:We describe the X-ray crystallographic structure of a murine T cell receptor (TCR) Valpha domain ("Valpha85.33"; AV11S5-AJ17) to 1.85 A resolution. The Valpha85.33 domain is derived from a TCR that recognizes a type II collagen peptide associated with the murine major histocompatibility complex (MHC) class II molecule...

journal_title：Journal of molecular biology

authors： Machius M,Cianga P,Deisenhofer J,Ward ES

更新日期：2001-07-20 00:00:00

abstract：:The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein-DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis...

journal_title：Journal of molecular biology

authors： Singh S,Plaks JG,Homa NJ,Amrich CG,Héroux A,Hatfull GF,VanDemark AP

更新日期：2014-01-23 00:00:00

abstract：:Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome a...

journal_title：Journal of molecular biology

authors： Jerinic O,Joseph S

更新日期：2000-12-15 00:00:00

abstract：:The second generation Hoechst-Bayer non-nucleoside inhibitor, HBY 097 (S-4-isopropoxycarbonyl-6-methoxy-3-(methylthiomethyl)-3, 4-dihydroqui noxalin-2(1H)-thione), is an extremely potent inhibitor of HIV-1 reverse transcriptase (RT) and of HIV-1 infection in cell culture. HBY 097 selects for unusual drug-resistance mu...

journal_title：Journal of molecular biology

authors： Hsiou Y,Das K,Ding J,Clark AD Jr,Kleim JP,Rösner M,Winkler I,Riess G,Hughes SH,Arnold E

更新日期：1998-11-27 00:00:00

abstract：:While the disulfide bridge is highly conserved within the immunoglobulin fold, a few antibody variable domains lack one of the essential cysteine residues. In the levan binding antibody ABPC48 one of the essential cysteine residues (Cys H92) of the heavy chain variable domain is replaced by tyrosine. We expressed scFv...

journal_title：Journal of molecular biology

authors： Proba K,Honegger A,Plückthun A

更新日期：1997-01-17 00:00:00