p53 accumulates but is functionally impaired when DNA synthesis is blocked.

abstract：:Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armache JP,Jarasch A,Anger AM,Villa E,Becker T,Bhushan S,Jossinet F,Habeck M,Dindar G,Franckenberg S,Marquez V,Mielke T,Thomm M,Berninghausen O,Beatrix B,Söding J,Westhof E,Wilson DN,Beckmann R

更新日期：2010-11-16 00:00:00

abstract：:Nanoparticle-based experimental therapeutics are currently being investigated in numerous human clinical trials. CALAA-01 is a targeted, polymer-based nanoparticle containing small interfering RNA (siRNA) and, to our knowledge, was the first RNA interference (RNAi)-based, experimental therapeutic to be administered to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuckerman JE,Gritli I,Tolcher A,Heidel JD,Lim D,Morgan R,Chmielowski B,Ribas A,Davis ME,Yen Y

更新日期：2014-08-05 00:00:00

abstract：:Seven morphologically nontransformed (flat) revertants with reduced tumorigenicity in vivo have been isolated from populations of Kirsten sarcoma virus-transformed NIH 3T3 cells transfected with a cDNA expression library of normal human fibroblasts. Each revertant harbors 1-10 recombinant plasmids per cell and retains...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noda M,Kitayama H,Matsuzaki T,Sugimoto Y,Okayama H,Bassin RH,Ikawa Y

更新日期：1989-01-01 00:00:00

abstract：:Proper segregation of chromosomes in meiosis is essential to prevent miscarriages and birth defects. This requires that sister chromatids maintain cohesion at the centromere as cohesion is released on the chromatid arms when the homologs segregate at anaphase I. The Shugoshin proteins preserve centromere cohesion by p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pinto BS,Orr-Weaver TL

更新日期：2017-12-05 00:00:00

abstract：:By using a structure-based computer-assisted search, we have found a butyrophenone derivative that is a selective inhibitor of the human immunodeficiency virus 1 (HIV-1) protease. The computer program creates a negative image of the active site cavity using the crystal structure of the HIV-1 protease. This image was c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DesJarlais RL,Seibel GL,Kuntz ID,Furth PS,Alvarez JC,Ortiz de Montellano PR,DeCamp DL,Babé LM,Craik CS

更新日期：1990-09-01 00:00:00

abstract：:The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important, previously unknown rol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao CH,Yao L,Xu Y,Liu WB,Zhou Y,Ye BC

更新日期：2015-12-22 00:00:00

abstract：:Infant acute lymphoblastic leukemia (ALL) with MLL gene rearrangements is characterized by early pre-B phenotype (CD10(-)/CD19(+)) and poor treatment outcome. The t(4;11), creating MLL-AF4 chimeric transcripts, is the predominant 11q23 chromosome translocation in infant ALL and is associated with extremely poor progno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taki T,Kano H,Taniwaki M,Sako M,Yanagisawa M,Hayashi Y

更新日期：1999-12-07 00:00:00

abstract：:A near-full-length casbene synthase cDNA clone, pCS7, was isolated by using a partial cDNA clone, pCS4, to probe a lambda gt10 library constructed from poly(A)+ RNA from elicited castor bean seedlings. The cDNA insert had a length of 1983 bases with a polyadenylate tail of 19 bases. Translation of the cDNA sequence re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mau CJ,West CA

更新日期：1994-08-30 00:00:00

abstract：:Although it is well described in model membranes, little is known about phase separation in biological membranes. Here, we provide evidence for a coexistence of at least two different lipid bilayer phases in the apical plasma membrane of epithelial cells. Phase connectivity was assessed by measuring long-range diffusi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meder D,Moreno MJ,Verkade P,Vaz WL,Simons K

更新日期：2006-01-10 00:00:00

abstract：:In monocots, many genes demonstrate a significant negative GC gradient, meaning that the GC content declines along the orientation of transcription. Such a gradient is not observed in the genes of the dicot plant Arabidopsis. In addition, a lack of homology is often observed when comparing the 5' end of the coding reg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang N,Ferguson AA,Slotkin RK,Lisch D

更新日期：2011-01-25 00:00:00

abstract：:Double fertilization is a key innovation for the evolutionary success of angiosperms by which the two fertilized female gametes, the egg cell and central cell, generate the embryo and endosperm, respectively. The female gametophyte (embryo sac) enclosed in the sporophyte is derived from a one-celled haploid cell linea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang MX,Zhu SS,Xu YC,Guo YL,Yang WC,Li HJ

更新日期：2020-03-17 00:00:00

abstract：:There is an important medical need for new antifungal agents with novel mechanisms of action to treat the increasing number of patients with life-threatening systemic fungal disease and to overcome the growing problem of resistance to current therapies. F901318, the leading representative of a novel class of drug, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JD,Sibley GEM,Beckmann N,Dobb KS,Slater MJ,McEntee L,du Pré S,Livermore J,Bromley MJ,Wiederhold NP,Hope WW,Kennedy AJ,Law D,Birch M

更新日期：2016-11-08 00:00:00

abstract：:A protein methylation reaction involved in chemotaxis of Escherichia coli has been identified. The involvement of this reaction in chemotaxis in indicated by four lines of evidence. (a) The methylation reaction is altered in several classes of generally nonchemotactic mutants and is coreverted with the chemotaxis defe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kort EN,Goy MF,Larsen SH,Adler J

更新日期：1975-10-01 00:00:00

abstract：:Amphibian eggs and embryos as well as mammalian cells have been reported to contain an activity that unwinds double-stranded RNA. We have now found that adenosine residues have been modified in the RNA products of this unwinding activity. Although the modified RNA remains double-stranded, the modification causes the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner RW,Smith JE,Cooperman BS,Nishikura K

更新日期：1989-04-01 00:00:00

abstract：:The nodZ gene, which is present in various soil bacteria such as Bradyrhizobium japonicum, Azorhizobium caulinodans, and Rhizobium loti, is involved in the addition of a fucosyl residue to the reducing N-acetylglucosamine residue of lipochitin oligosaccharide (LCO) signal molecules. Using an Escherichia coli strain th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quinto C,Wijfjes AH,Bloemberg GV,Blok-Tip L,López-Lara IM,Lugtenberg BJ,Thomas-Oates JE,Spaink HP

更新日期：1997-04-29 00:00:00

abstract：:A randomized double-blind controlled trial was conducted to determine the efficacy of passive immunotherapy in the treatment of symptomatic human immunodeficiency virus (HIV) infection. This trial included 86 symptomatic patients randomized to receive plasma rich in anti-HIV-1 antibody or standard seronegative plasma....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vittecoq D,Chevret S,Morand-Joubert L,Heshmati F,Audat F,Bary M,Dusautoir T,Bismuth A,Viard JP,Barré-Sinoussi F

更新日期：1995-02-14 00:00:00

abstract：:We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient with cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and express cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoumacher RA,Ram J,Iannuzzi MC,Bradbury NA,Wallace RW,Hon CT,Kelly DR,Schmid SM,Gelder FB,Rado TA

更新日期：1990-05-01 00:00:00

abstract：:Clinically available drugs active against Epstein-Barr virus (EBV) and other human herpesviruses are limited to those targeting viral DNA replication. To identify compounds directed against other steps in the viral life cycle, we searched for drugs active against the EBV SM protein, which is essential for infectious v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verma D,Thompson J,Swaminathan S

更新日期：2016-03-29 00:00:00

abstract：:The 2-deoxy-D-glucose method was used to study asymmetries in cerebral metabolic activity in neonatal rats. Left-right asymmetries in 2-deoxy-D-glucose uptake were observed in hippocampus, diencephalon, cortex, and medulla-pons: 2-deoxy-D-glucose incorporation was greater in right hippocampus, right diencephalon, left...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ross DA,Glick SD,Meibach RC

更新日期：1981-03-01 00:00:00

abstract：:Androgen independence is responsible for most prostate cancer lethality, yet currently there are no effective clinical treatments. We have been investigating the mechanisms underlying androgen-independent prostate cancer in Nkx3.1;Pten mutant mice, which display salient features of the disease, including a requirement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao H,Ouyang X,Banach-Petrosky WA,Gerald WL,Shen MM,Abate-Shen C

更新日期：2006-09-26 00:00:00

abstract：:Results on the subcellular localization of the mineralocorticoid receptor (MR) have been controversial. To determine the subcellular distribution and trafficking of the MR in living cells after binding of agonists and antagonists, we expressed a MR-green fluorescent protein (GFP) chimera in mammalian cells lacking end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fejes-Tóth G,Pearce D,Náray-Fejes-Tóth A

更新日期：1998-03-17 00:00:00

abstract：:Bone morphogenetic protein 4 (BMP-4) induces ventral mesoderm but represses dorsal mesoderm formation in Xenopus embryos. We show that BMP-4 inhibits two signaling pathways regulating dorsal mesoderm formation, the induction of dorsal mesoderm (Spemann organizer) and the dorsalization of ventral mesoderm. Ectopic expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Re'em-Kalma Y,Lamb T,Frank D

更新日期：1995-12-19 00:00:00

abstract：:We have isolated from vaccinia virus cores an enzyme, 5'-phosphate-polyribonucleotide kinase, that in the presence of ATP and Mg2+ catalyzes the conversion of 5'-phosphate and 5'-diphosphate termini of RNA to the 5'-triphosphate species. With the exception of dATP, other nucleoside triphosphates were inactive as phosp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spencer E,Loring D,Hurwitz J,Monroy G

更新日期：1978-10-01 00:00:00

abstract：:In the course of scrapie, a transmissible spongiform encephalopathy caused by an unconventional agent, a normal cellular protein is converted to an abnormal form that copurifies with infectivity and aggregates to form deposits of amyloid. We have used immunocytochemistry and methods that enhance detection of amyloidog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diedrich JF,Bendheim PE,Kim YS,Carp RI,Haase AT

更新日期：1991-01-15 00:00:00

abstract：:Meningococcal meningitis remains a substantial cause of mortality and morbidity worldwide. Until recently, countries in the African meningitis belt were susceptible to devastating outbreaks, largely attributed to serogroup A Neisseria meningitidis (MenA). Vaccination with glycoconjugates of MenA capsular polysaccharid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henriques P,Dello Iacono L,Gimeno A,Biolchi A,Romano MR,Arda A,Bernardes GJL,Jimenez-Barbero J,Berti F,Rappuoli R,Adamo R

更新日期：2020-11-24 00:00:00

abstract：:In photosynthetic systems, the absorbed light energy is used to generate electron transport or it is lost in the form of fluorescence and thermal emission. While fluorescence can be readily measured, the detection of thermal deactivation processes can be achieved by the photoacoustic technique. In that case, the press...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Allakhverdiev SI,Klimov VV,Carpentier R

更新日期：1994-01-04 00:00:00

abstract：:In nine patients with GM1 gangliosidosis, liver ganglioside GM1 beta-galactosidase (EC 3.2.1.23) activity ranged from less than 0.01% to 0.05% of normal. In a tenth patient's liver, much higher activity was found (0.5% of normal). In this patient the residual enzyme had the same molecular weight as beta-galactosidase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Norden AG,O'Brien JS

更新日期：1975-01-01 00:00:00

abstract：:The type II cell of the alveolar epithelium of adult rats has been studied by electron microscopy to determine its ability to endocytose electron-dense tracers of differing molecular charge and size. The tracers studied were native (anionic) ferritin, cationic ferritin, 70-kilodalton dextran, and colloidal carbon. Alv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams MC

更新日期：1984-10-01 00:00:00

abstract：:RAG1 and RAG2 are lymphoid-specific genes that together induce V(D)J recombinase activity in a variety of nonlymphoid cell types. While no other lymphoid-specific factors are required to induce recombination, other factors with more widespread expression patterns have been implicated in the reaction. However, none of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuomo CA,Kirch SA,Gyuris J,Brent R,Oettinger MA

更新日期：1994-06-21 00:00:00

abstract：:A goal of regenerative medicine is to identify cardiovascular progenitors from human ES cells (hESCs) that can functionally integrate into the human heart. Previous studies to evaluate the developmental potential of candidate hESC-derived progenitors have delivered these cells into murine and porcine cardiac tissue, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ardehali R,Ali SR,Inlay MA,Abilez OJ,Chen MQ,Blauwkamp TA,Yazawa M,Gong Y,Nusse R,Drukker M,Weissman IL

更新日期：2013-02-26 00:00:00