Abstract:
:We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers, PLL-g-[(PEGm)((1-x)) (PEG-biotin)(x)], where x varies from 0 to 1. Monolayers were produced on titanium dioxide substrates and characterized by x-ray photoelectron spectroscopy. The specific biorecognition properties of these biotinylated surfaces were investigated with the use of radiolabeled streptavidin alone and within complex protein mixtures. The PLL-g-PEG-biotin monolayers specifically capture streptavidin, even from a complex protein mixture, while still preventing nonspecific adsorption of other proteins. This streptavidin layer can subsequently capture biotinylated proteins. Finally, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this class of biomolecular interfaces for applications based on protein patterning.
journal_name
Proc Natl Acad Sci U S Aauthors
Ruiz-Taylor LA,Martin TL,Zaugg FG,Witte K,Indermuhle P,Nock S,Wagner Pdoi
10.1073/pnas.98.3.852keywords:
subject
Has Abstractpub_date
2001-01-30 00:00:00pages
852-7issue
3eissn
0027-8424issn
1091-6490pii
98/3/852journal_volume
98pub_type
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