Abstract:
:129/SvEv mice with a loss-of-function mutation in the heterotrimeric G protein α-subunit gene Gnai3 have fusions of ribs and lumbar vertebrae, indicating a requirement for Gα(i) (the "inhibitory" class of α-subunits) in somite derivatives. Mice with mutations of Gnai1 or Gnai2 have neither defect, but loss of both Gnai3 and one of the other two genes increases the number and severity of rib fusions without affecting the lumbar fusions. No myotome defects are observed in Gnai3/Gnai1 double-mutant embryos, and crosses with a conditional allele of Gnai2 indicate that Gα(i) is specifically required in cartilage precursors. Penetrance and expressivity of the rib fusion phenotype is altered in mice with a mixed C57BL/6 × 129/SvEv genetic background. These phenotypes reveal a previously unknown role for G protein-coupled signaling pathways in development of the axial skeleton.
journal_name
Proc Natl Acad Sci U S Aauthors
Plummer NW,Spicher K,Malphurs J,Akiyama H,Abramowitz J,Nürnberg B,Birnbaumer Ldoi
10.1073/pnas.1219810110subject
Has Abstractpub_date
2012-12-26 00:00:00pages
21366-71issue
52eissn
0027-8424issn
1091-6490pii
1219810110journal_volume
109pub_type
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