Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library.

abstract：:Transfer RNA is one of the most richly modified biological molecules. Biosynthetic pathways that introduce these modifications are underexplored, largely because their absence does not lead to obvious phenotypes under normal growth conditions. Queuosine (Q) is a hypermodified base found in the wobble positions of tRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles ZD,McCarty RM,Molnar G,Bandarian V

更新日期：2011-05-03 00:00:00

abstract：:Superhydrophobic surfaces display water contact angles greater than 150 degrees in conjunction with low contact angle hysteresis. Microscopic pockets of air trapped beneath the water droplets placed on these surfaces lead to a composite solid-liquid-air interface in thermodynamic equilibrium. Previous experimental and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tuteja A,Choi W,Mabry JM,McKinley GH,Cohen RE

更新日期：2008-11-25 00:00:00

abstract：:Exposure to childhood trauma (CT) increases the risk for psychosis and affects the development of brain structures, possibly through oxidative stress. As oxidative stress is also linked to psychosis, it may interact with CT, leading to a more severe clinical phenotype. In 133 patients with early psychosis (EPP), we ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alameda L,Fournier M,Khadimallah I,Griffa A,Cleusix M,Jenni R,Ferrari C,Klauser P,Baumann PS,Cuenod M,Hagmann P,Conus P,Do KQ

更新日期：2018-12-04 00:00:00

abstract：:Bacterial adaptation to nutrient limitation and increased population densities is central to survival and virulence. Surprisingly, <3% of Escherichia coli genes are known to play roles specific to the stationary phase. There is evidence that the leucine-responsive regulatory protein (Lrp) may play an important role in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tani TH,Khodursky A,Blumenthal RM,Brown PO,Matthews RG

更新日期：2002-10-15 00:00:00

abstract：:Cell-specific activation of the transcription factor sigma F during sporulation in Bacillus subtilis is controlled by a regulatory pathway involving the proteins SpoIIE, SpoIIAA, and SpoIIAB. SpoIIAB is an antagonist of sigma F, and SpoIIAA, which is capable of overcoming SpoIIAB-mediated inhibition of sigma F, is an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arigoni F,Duncan L,Alper S,Losick R,Stragier P

更新日期：1996-04-16 00:00:00

abstract：:Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schreiber AB,Kenney J,Kowalski WJ,Friesel R,Mehlman T,Maciag T

更新日期：1985-09-01 00:00:00

abstract：:We previously reported that KIF3A and KIF3B form a heterodimer that functions as a microtubule-based fast anterograde translocator of membranous organelles. We have also shown that this KIF3A/3B forms a complex with other associated polypeptides, named kinesin superfamily-associated protein 3 (KAP3). In the present st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamazaki H,Nakata T,Okada Y,Hirokawa N

更新日期：1996-08-06 00:00:00

abstract：:Preference behaviors are often established during early life, but the underlying neural circuit mechanisms remain unknown. Adapting a unique nesting behavior assay, we confirmed a "critical period" for developing music preference in C57BL/6 mice. Early music exposure between postnatal days 15 and 24 reversed their inn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang EJ,Lin EW,Hensch TK

更新日期：2012-10-16 00:00:00

abstract：:Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive (42)K and (86)Rb by a temperature- and energy-dependent process. Memb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharyya P,Epstein W,Silver S

更新日期：1971-07-01 00:00:00

abstract：:The RNA world hypothesis presumes that RNA will be competent for varied essential cellular functions. One such indispensable cell function is regulation of membrane permeability. Though this was not a known RNA activity, selection-amplification yielded RNAs that bound phosphatidylcholine:cholesterol liposomes. At leas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khvorova A,Kwak YG,Tamkun M,Majerfeld I,Yarus M

更新日期：1999-09-14 00:00:00

abstract：:Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous neuropeptide ligands for the G protein-coupled receptors NPBWR1 and NPBWR2. Here we report that the majority of NPW neurons in the mesolimbic region possess tyrosine hydroxylase immunoreactivity, indicating that a small subset of dopaminergic neurons coexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Motoike T,Long JM,Tanaka H,Sinton CM,Skach A,Williams SC,Hammer RE,Sakurai T,Yanagisawa M

更新日期：2016-05-24 00:00:00

abstract：:Modern European genetic structure demonstrates strong correlations with geography, while genetic analysis of prehistoric humans has indicated at least two major waves of immigration from outside the continent during periods of cultural change. However, population-level genome data that could shed light on the demograp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Veeramah KR,Rott A,Groß M,van Dorp L,López S,Kirsanow K,Sell C,Blöcher J,Wegmann D,Link V,Hofmanová Z,Peters J,Trautmann B,Gairhos A,Haberstroh J,Päffgen B,Hellenthal G,Haas-Gebhard B,Harbeck M,Burger J

更新日期：2018-03-27 00:00:00

abstract：:Visual development depends on sensory input during an early developmental critical period. Deviation of the pointing direction of the two eyes (strabismus) or chronic optical blur (anisometropia) separately and together can disrupt the formation of normal binocular interactions and the development of spatial processin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou C,Pettet MW,Norcia AM

更新日期：2014-07-29 00:00:00

abstract：:Plant mortality and birth rates are critical components of plant life history affecting the stability of plant populations and the ecosystems they form. Although allometric theory predicts that both plant birth and mortality rates should be size-dependent, this prediction has not yet been tested across plants ranging ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marbà N,Duarte CM,Agustí S

更新日期：2007-10-02 00:00:00

abstract：:MurG is an essential glycosyltransferase that forms the glycosidic linkage between N-acetyl muramyl pentapeptide and N-acetyl glucosamine in the biosynthesis of the bacterial cell wall. This enzyme is a member of a major superfamily of NDP-glycosyltransferases for which no x-ray structures containing intact substrates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Chen L,Ha S,Gross B,Falcone B,Walker D,Mokhtarzadeh M,Walker S

更新日期：2003-02-04 00:00:00

abstract：:Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated by microinjection an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillip Y,Kiss V,Schreiber G

更新日期：2012-01-31 00:00:00

abstract：:Insertion of the transposon Tn5 into the T-region of the octopine Ti plasmid of Agrobacterium tumefaciens gives rise to crown gall tumors having altered morphology. Three loci within the T-DNA that control tumor morphology have been detected [Garfinkel, D. J., Simpson, R. B., Ream, L. W., White, F. F., Gordon, M. P. &...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyoshi DE,Morris RO,Hinz R,Mischke BS,Kosuge T,Garfinkel DJ,Gordon MP,Nester EW

更新日期：1983-01-01 00:00:00

abstract：:Once-abundant aerial insectivores, such as the Tree Swallow (Tachycineta bicolor), have declined steadily in the past several decades, making it imperative to understand all aspects of their ecology. Aerial insectivores forage on a mixture of aquatic and terrestrial insects that differ in fatty acid composition, speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Twining CW,Brenna JT,Lawrence P,Shipley JR,Tollefson TN,Winkler DW

更新日期：2016-09-27 00:00:00

abstract：:We have isolated, cloned, and expressed a male antennae-specific pheromone-degrading enzyme (PDE) [Antheraea polyphemus PDE (ApolPDE), formerly known as Sensillar Esterase] from the wild silkmoth, A. polyphemus, which seems essential for the rapid inactivation of pheromone during flight. The onset of enzymatic activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida Y,Leal WS

更新日期：2005-09-27 00:00:00

abstract：:A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raderschall E,Golub EI,Haaf T

更新日期：1999-03-02 00:00:00

abstract：:This paper is the second of three describing a two-fluid theory of binary liquid mixtures. The general theory presented in the preceding paper is used to derive a model for calculating thermodynamic properties of hard-sphere mixtures. Calculations indicate that desirable boundary conditions are satisfied. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brandani V,Prausnitz JM

更新日期：1982-08-01 00:00:00

abstract：:Social behavior is often shaped by the rich storehouse of biographical information that we hold for other people. In our daily life, we rapidly and flexibly retrieve a host of biographical details about individuals in our social network, which often guide our decisions as we navigate complex social interactions. Even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Collins JA,Koski J,Nugiel T,Metoki A,Olson IR

更新日期：2017-04-18 00:00:00

abstract：:We have used restriction endonuclease mapping of cell DNA to investigate the structure of the beta-globin gene in beta-thalassemias. Among 17 individuals with beta +- and beta 0-thalassemia, we observed three patients of Indian origin with beta 0-thalassemia whose DNA revealed a consistent mapping abnormality. In one ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orkin SH,Old JM,Weatherall DJ,Nathan DG

更新日期：1979-05-01 00:00:00

abstract：:Numerous relatively short regions within intrinsically disordered proteins (IDPs) serve as molecular recognition elements (MoREs). They fold into ordered structures upon binding to their partner molecules. Currently, there is still a lack of in-depth understanding of how coupled binding and folding occurs in MoREs. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Chu X,Longhi S,Roche P,Han W,Wang E,Wang J

更新日期：2013-10-01 00:00:00

abstract：:The intestinal mucosa exists in dynamic balance with trillions of luminal microbes. Disruption of the intestinal epithelial barrier, commonly observed in mucosal inflammation and diseases such as inflammatory bowel diseases (IBDs), is often associated with dysbiosis, particularly decreases in species producing short-c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang RX,Lee JS,Campbell EL,Colgan SP

更新日期：2020-05-26 00:00:00

abstract：:After intravitreal injection of gamma-[3H] aminobutyric acid (GAB), 2% of the neurons at the outer margin of the inner plexiform layer were intensely labeled. Reconstructions of these neurons from serial electron microscope autoradiograms showed that they are interplexiform cells, which synapse on bipolar processes in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,McGuire BA,Sterling P

更新日期：1980-01-01 00:00:00

abstract：:Ligand-specific molecular switches composed of RNA were created by coupling preexisting catalytic and receptor domains via structural bridges. Binding of ligand to the receptor triggers a conformational change within the bridge, and this structural reorganization dictates the activity of the adjoining ribozyme. The mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soukup GA,Breaker RR

更新日期：1999-03-30 00:00:00

abstract：:Human beings differ in their ability to master the sounds of their second language (L2). Phonetic training studies have proposed that differences in phonetic learning stem from differences in psychoacoustic abilities rather than speech-specific capabilities. We aimed at finding the origin of individual differences in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Díaz B,Baus C,Escera C,Costa A,Sebastián-Gallés N

更新日期：2008-10-21 00:00:00

abstract：:Superficial cells from early amphibian embryos display regional specializations of their cell surfaces. That portion of the plasma membrane facing the perivitelline space (apical surface) is nonadhesive, whereas, in the same cell, the lateral and basal portions of the plasma membrane will adhere to other cells. These ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberson MM,Armstrong PB

更新日期：1980-06-01 00:00:00

abstract：:In movies or on TV, a wheel can seem to rotate backwards, due to the temporal subsampling inherent in the recording process (the wagon wheel illusion). Surprisingly, this effect has also been reported under continuous light, suggesting that our visual system, too, might sample motion in discrete "snapshots." Recently,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanRullen R,Reddy L,Koch C

更新日期：2005-04-05 00:00:00