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Control of chondrogenic expression in mesodermal cells of embryonic chick limb.

Abstract：

:Chick-limb mesodermal cells have been maintained in tissue culture under conditions that permit development of muscle and cartilage. 3-Acetylpyridine, a nicotinamide-antagonized muscle teratogen, potentiates chondrogenic expression in cell cultures, as evidenced by histological and biochemical criteria, including the production of chondromucoprotein. Xylosyltransferase and N-acetylgalactosaminyltransferase are two enzymes required for chondromucoprotein synthesis; the specific activities of these enzymes were measured in differentiating mesodermal cells cultured for various periods of time in the presence and absence of 3-acetylpyridine and in intact limb tissue. The ratios of specific enzyme activities were nearly the same in both cultured cells and limb tissue, although the levels of both transferases increased severalfold during chondrogenic expression by mesodermal cells in culture. 3-Acetylpyridine causes the specific activities of xylosyl- and N-acetylgalactosaminyltransferases to increase 10- and 2-fold, respectively, above those of untreated cultures. Compared to the ratio of transferase activities in limb tissue and differentiating cell cultures, 3-acetylpyridine appears to increase the activity of xylosyltransferase, the initiator of chondroitin sulfate chain synthesis, more than does N-acetylgalactosaminyltransferase; this finding implies that the synthesis of these two enzymes may be separately regulated.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Caplan AI,Stoolmiller AC

doi

10.1073/pnas.70.6.1713

subject

Has Abstract

pub_date

1973-06-01 00:00:00

pages

1713-7

issue

6

eissn

0027-8424

issn

1091-6490

journal_volume

70

pub_type

杂志文章

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