Abstract：
:The amplification of the STR DYS391, using the primers described in the Genome Data Base (GDB: G00-365-251), shows not only an additional band to the Y-specific one in males with a size range of 26 bp less than those of DYS391 locus alleles, but also a polymorphic pattern in females in the same size range as the additional band observed in males. The DYS391 pattern in families reflects a Y-specific linked locus and also a polymorphic X locus with an X-linked pattern of inheritance. A first screening in the X homologous locus allowed the identification of five different alleles. Allele frequencies were explored in different population groups for both the Y locus and the homologous locus in the X chromosome showing a similar allele distribution pattern in the X and Y homologous loci. An alternative reverse primer was designed to amplify the Y-chromosome specific STR in order to improve the specificity and applicability of this system to forensic genetics. Comparative results of the amplification with the new and the previously described primers proved that with this new primer there is a substantial increase in the specificity of the amplification. Moreover, a smaller fragment is amplified with a size out of the range of the alleles of the other Y-STRs usually used in forensic applications, therefore simplifying its inclusion in multiplex systems.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Gusmão L,González-Neira A,Sánchez-Diz P,Lareu MV,Amorim A,Carracedo Adoi
10.1016/s0379-0738(00)00171-7keywords:
subject
Has Abstractpub_date
2000-07-24 00:00:00pages
49-57issue
1eissn
0379-0738issn
1872-6283pii
S0379073800001717journal_volume
112pub_type
杂志文章abstract：:Chemical and physicochemical examinations of the fragments of an Egyptian mummy dated between the 3rd and 1st century B.C. were performed. The chemical examinations indicated the presence of resin in the skull and in the fragments of bandages wrapped around the mummy. An analysis of the infrared spectra of the fragmen...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/s0379-0738(98)00192-3
更新日期：1999-01-25 00:00:00
abstract：:The present study investigated the interobserver variation between a radiologist and a forensic pathologist in 994 injury diagnoses obtained by postmortem computed tomography (CT) of 67 traffic fatality victims, and the results were compared with diagnoses obtained by autopsy. The injuries were coded according to the ...
journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.03.012
更新日期：2007-07-04 00:00:00
abstract：:A study of the quantities of cocaine on banknotes in general circulation was conducted to investigate regional variations across England and Wales. No meaningful support was found for the proposition that there is regional variation in the quantities of cocaine in banknotes in general circulation in England and Wales....
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.10.017
更新日期：2017-01-01 00:00:00
abstract：:Despite indicating numerous demographic features such as sex, stature, and age; the sacrum remains a relatively insufficiently researched skeletal element. A set pattern of ossification and fusion of the sacrum makes it a useful bone for estimating age-at-death in unknown skeletal individuals. The aims of this study, ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.02.011
更新日期：2018-05-01 00:00:00
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journal_title：Forensic science international
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.12.039
更新日期：2018-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.11.004
更新日期：2019-01-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/S0379-0738(03)00181-6
更新日期：2004-02-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.04.022
更新日期：2014-07-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2018.07.007
更新日期：2018-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(85)90173-2
更新日期：1985-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.11.002
更新日期：2014-01-01 00:00:00
abstract：:The Association of Firearm and Toolmark Examiners recommends a minimum of two test fires be performed when an unknown firearm is submitted to a laboratory prior to doing a comparison with a cartridge case collected from a crime scene. Limited research has been performed to determine how many test fires are necessary t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.06.010
更新日期：2018-09-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.03.011
更新日期：2011-05-20 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.06.003
更新日期：2011-10-10 00:00:00
abstract：:It is well-known that secular trends affect human stature and constitution, and this fact should be taken into consideration in forensic anthropology, especially in stature estimation. Recently, stature-group-specific equations have been developed to take into account these variations. The aim of the present study is ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109973
更新日期：2019-11-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.03.007
更新日期：2013-05-10 00:00:00
abstract：:Forensic assessment of skeletal material includes age estimation of unknown individuals. When dealing with extremely fragmented human remains that lack macro-features used in age estimation, histological assessment of the skeletal elements can be employed. Historically, microscopic methods for age assessment used by f...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110335
更新日期：2020-08-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00384-4
更新日期：2001-06-01 00:00:00
abstract：:In Switzerland, the sale of cannabis with tetrahydrocannabinol (THC) content less than 1% has recently been legalized. As a consequence, cannabis with low THC and high cannabidiol (CBD) values up to approximately 25% is legally available on the market. In this study, we investigated cannabinoid blood and urine concent...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.08.009
更新日期：2018-10-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.09.001
更新日期：2014-11-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109932
更新日期：2019-10-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.12.044
更新日期：2016-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.02.042
更新日期：2019-05-01 00:00:00
abstract：:Allele frequencies for seven STRs loci were obtained from a sample of 215 unrelated healthy Italian individuals. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00513-8
更新日期：2001-11-15 00:00:00
abstract：:Analysis of cannabis has gained new importance worldwide, mainly for quality control within the legalized recreational and medical cannabis industry, but also for forensic differentiation between drug-type cannabis and legal products such as fiber hemp and CBD-rich/THC-poor cannabis. We herein present an HPLC-DAD meth...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.03.046
更新日期：2019-06-01 00:00:00
abstract：:During the prosecution and defence of drink-driving cases, forensic practitioners are often required to engage in various blood-alcohol calculations, such as whether or not the statutory limit was exceeded (e.g. 80mg/100mL, 0.08g/100mL or 0.80g/L). For this purpose, most forensic scientists utilize the Widmark equatio...
journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
abstract：INTRODUCTION:The use of a LC-MS/MS system for benzodiazepines detection remarkably increased the analytical sensitivity of these drugs in biological matrices, in particular in non-conventional ones such as hair. Since the amount of hair sample available for the analysis is frequently limited and, moreover, it needs to ...
journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2012-05-10 00:00:00
abstract：:The discovery in 1934 of the badly burned, pyjama clad body of a young woman protruding from a roadside culvert near Albury, N.S.W., led to one of the largest investigations in the history of Australian crime. Two simple errors, made by a local dentist with no previous experience in forensic odontology called by the p...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(82)90110-4
更新日期：1982-07-01 00:00:00
