Antigen-presenting cells and keratinocytes express interleukin-12 in allergic contact dermatitis.

Abstract:

:Interleukin-12 (IL-12) has previously been suggested as playing a major rôle in the activation of cytotoxic lymphocytes. Recent reports indicate that cytotoxic CD8+ cells are critically involved in the elicitation phase of contact hypersensitivity reactions. In this study, the in situ expression of IL-12 was investigated in normal human skin and in allergic contact dermatitis by immunohistochemistry. Skin biopsy specimens were obtained from allergic patch test reactions after 3 days, and from normal skin in 8 subjects. In contrast to normal skin, a strong enhancement of IL-12 immunoreactivity was observed in the mononuclear cell infiltrate of allergic contact dermatitis. IL-12 immunoreactivity was mainly located in the cytoplasm of dermal dendritic cells and macrophages as well as of some Langerhans cells. IL-12-positive cells were often found in close apposition to lymphocytes. Furthermore, positive immunostaining was also detected in keratinocytes at sites of marked exocytosis and spongiosis in the epidermis. In conclusion, the enhanced in situ expression of IL-12 may contribute to the activation of cytotoxic lymphocytes and thereby represent an important factor in the pathogenesis of contact hypersensitivity reactions in humans.

journal_name

Contact Dermatitis

journal_title

Contact dermatitis

authors

Yawalkar N,Egli F,Brand CU,Pichler WJ,Braathen LR

doi

10.1034/j.1600-0536.2000.042001018.x

keywords:

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

18-22

issue

1

eissn

0105-1873

issn

1600-0536

journal_volume

42

pub_type

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