Purification and characterization of the retina-specific cell-aggregating factor.

abstract：:Antibodies to the insulin receptor were prepared in BALB/c mice by immunization with IM-9 human lymphocytes, a cell type that has a large number of plasma membrane insulin receptors. The spleens of these mice were then removed, and their lymphocytes were fused to a mouse myeloma cell line, FO cells. After screening ov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roth RA,Cassell DJ,Wong KY,Maddux BA,Goldfine ID

更新日期：1982-12-01 00:00:00

abstract：:The alternative adaptive immune system of jawless vertebrates is based on different isotypes of variable lymphocyte receptors (VLRs) that are composed of leucine-rich repeats (LRRs) and expressed by distinct B- and T-like lymphocyte lineages. VLRB is expressed by B-like cells, whereas VLRA and VLRC are expressed by tw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holland SJ,Gao M,Hirano M,Iyer LM,Luo M,Schorpp M,Cooper MD,Aravind L,Mariuzza RA,Boehm T

更新日期：2014-10-14 00:00:00

abstract：:Induction, maintenance, and amplification of tumor-protective immunity after cytokine gene therapy is essential for the clinical success of immunotherapeutic approaches. We investigated whether this could be achieved by single-chain IL-12 (scIL-12) gene therapy followed by tumor-targeted IL-2 using a fusion protein co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lode HN,Xiang R,Duncan SR,Theofilopoulos AN,Gillies SD,Reisfeld RA

更新日期：1999-07-20 00:00:00

abstract：:Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu TL,Hanson SR,Kishikawa K,Wang SK,Sawa M,Wong CH

更新日期：2007-02-20 00:00:00

abstract：:Although the apoptotic role of caspases has been largely understood, accumulating evidence in Drosophila suggests that caspases also control other processes than apoptotic cell death. However, how caspases contribute to the development of the mammalian nervous system remains obscure. Here, we provide unique evidence t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohsawa S,Hamada S,Kuida K,Yoshida H,Igaki T,Miura M

更新日期：2010-07-27 00:00:00

abstract：:We have previously shown that in neutrophils deprived of granulocyte colony-stimulating factor, apoptosis is preceded by acidification and that the protection against apoptosis conferred on neutrophils by granulocyte colony-stimulating factor is dependent upon delay of this acidification. To test the hypothesis that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gottlieb RA,Nordberg J,Skowronski E,Babior BM

更新日期：1996-01-23 00:00:00

abstract：:Compared with understanding of biological shape and form, knowledge is sparse regarding what regulates growth and body size of a species. For example, the genetic and physiological causes of heterosis (hybrid vigor) have remained elusive for nearly a century. Here, we investigate gene-expression patterns underlying gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hedgecock D,Lin JZ,DeCola S,Haudenschild CD,Meyer E,Manahan DT,Bowen B

更新日期：2007-02-13 00:00:00

abstract：:This paper investigates quantitatively the characteristics of the local folding code. The overlapping four-residue fragments which make up the amino acid sequences of 114 proteins are divided into classes on the basis of the physical properties of their constituent amino acids. The distribution of structural types ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S

更新日期：1993-01-15 00:00:00

abstract：:The immunophilin-immunosuppressant complexes cyclophilin-cyclosporin A (CsA) and FKBP12-FK506 inhibit the phosphatase calcineurin to block T-cell activation. Although cyclophilin A, FKBP12, and calcineurin are highly conserved from yeast to man, none had previously been shown to be essential for viability. We find tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breuder T,Hemenway CS,Movva NR,Cardenas ME,Heitman J

更新日期：1994-06-07 00:00:00

abstract：:Glucocorticoid hormones increase the level of hepatic tryptophan oxygenase (EC 1.13.11.11; L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing) by increasing its rate of synthesis. Studies were performed to determine whether this induction is mediated by controlling the level of the mRNA for tryptophan oxygenase of by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schutz G,Killewich L,Chen G,Feigelson P

更新日期：1975-03-01 00:00:00

abstract：:Borrelia burgdorferi (Bb), the causative agent of Lyme disease, is transmitted to mammalian hosts through an arthropod (tick) vector. To establish infection, Bb must acquire essential nutrients, including transition metals, from its mammalian and tick hosts. Thus far, no metal transporter has been identified in Bb. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouyang Z,He M,Oman T,Yang XF,Norgard MV

更新日期：2009-03-03 00:00:00

abstract：:The nuclear envelope is a unique topological structure formed by lipid membranes in eukaryotic cells. Unlike other membrane structures, the nuclear envelope comprises two concentric membrane shells fused at numerous sites with toroid-shaped pores that impart a "geometric" genus on the order of thousands. Despite the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torbati M,Lele TP,Agrawal A

更新日期：2016-10-04 00:00:00

abstract：:Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (SAH) or 5'-deoxyadenosine (5'-dAdo) and l-methionine (l-Met). Viperin c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenwick MK,Li Y,Cresswell P,Modis Y,Ealick SE

更新日期：2017-06-27 00:00:00

abstract：:Atrial natriuretic factor (ANF) is a group of peptides, originally isolated from the cardiac atria, that have a number of important effects on blood pressure, renal function, and salt balance. In the current study, expression of the ANF gene in certain extra-atrial tissues of the rat has been examined by radioimmunoas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gardner DG,Deschepper CF,Ganong WF,Hane S,Fiddes J,Baxter JD,Lewicki J

更新日期：1986-09-01 00:00:00

abstract：:Inherited single gene defects have been identified in both humans and mice that lead to loss of developmental control over the left-right asymmetry of the heart and viscera. In mice the recessively inherited mutation iv leads to such apparent loss of control over situs: 50% of iv/iv mice exhibit situs inversus and 50%...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brueckner M,D'Eustachio P,Horwich AL

更新日期：1989-07-01 00:00:00

abstract：:Pseudohypoaldosteronism type II is a salt-sensitive form of hypertension with hyperkalemia in humans caused by mutations in the with-no-lysine kinase 4 (WNK4). Several studies have shown that WNK4 modulates the activity of the renal Na(+)Cl(-) cotransporter, NCC. Because the renal consequences of WNK4 carrying pseudoa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castañeda-Bueno M,Cervantes-Pérez LG,Vázquez N,Uribe N,Kantesaria S,Morla L,Bobadilla NA,Doucet A,Alessi DR,Gamba G

更新日期：2012-05-15 00:00:00

abstract：:The sources of human germline mutations are poorly understood. Part of the difficulty is that mutations occur very rarely, and so direct pedigree-based approaches remain limited in the numbers that they can examine. To address this problem, we consider the spectrum of low-frequency variants in a dataset (Genome Aggreg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal I,Przeworski M

更新日期：2019-09-03 00:00:00

abstract：:Tritiated photoaffinity analogs of the natural lepidopteran juvenile hormones, JH I and II [epoxy[3H]bishomofarnesyl diazoacetate ([3H]EBDA) and epoxy[3H]homofarnesyl diazoacetate ([3H]EHDA)], and of the JH analog methoprene [[3H]methoprene diazoketone ([3H]MDK)] were synthesized and used to identify specific JH bindi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palli SR,Osir EO,Eng W,Boehm MF,Edwards M,Kulcsar P,Ujvary I,Hiruma K,Prestwich GD,Riddiford LM

更新日期：1990-01-01 00:00:00

abstract：:Depression is a multifactorial illness and genetic factors play a role in its etiology. The understanding of its physiopathology relies on the availability of experimental models potentially mimicking the disease. Here we describe a model built up by selective breeding of mice with strikingly different responses in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Yacoubi M,Bouali S,Popa D,Naudon L,Leroux-Nicollet I,Hamon M,Costentin J,Adrien J,Vaugeois JM

更新日期：2003-05-13 00:00:00

abstract：:An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the Kd is 6.9 microM, whereas for binding to ribosomes it is 0.7 microM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is in complexes w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Munishkin A,Wool IG

更新日期：1997-11-11 00:00:00

abstract：:We report an approach for studying protein-induced DNA bends in solution that is based on measuring the sizes of circular DNA molecules by using two-dimensional gel electrophoresis. These circular fragments are obtained by ligating short synthetic oligonucleotides containing a protein-recognition region in the presenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyubchenko Y,Shlyakhtenko L,Chernov B,Harrington RE

更新日期：1991-06-15 00:00:00

abstract：:Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although the direct observation of the fundamental processes is lacking. By combining rational peptide design with topographical (atomic force microsc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rakowska PD,Jiang H,Ray S,Pyne A,Lamarre B,Carr M,Judge PJ,Ravi J,Gerling UI,Koksch B,Martyna GJ,Hoogenboom BW,Watts A,Crain J,Grovenor CR,Ryadnov MG

更新日期：2013-05-28 00:00:00

abstract：:Basophils are powerful mediators of Th2 immunity and are present in increased numbers during allergic inflammation and helminth infection. Despite their ability to potentiate Th2 immunity the mechanisms regulating basophil development remain largely unknown. We have found a unique role for isotype-switched antibodies ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herbst T,Esser J,Prati M,Kulagin M,Stettler R,Zaiss MM,Hewitson JP,Merky P,Verbeek JS,Bourquin C,Camberis M,Prout M,Maizels RM,Le Gros G,Harris NL

更新日期：2012-09-11 00:00:00

abstract：:Caenorhabditis elegans sqv mutants are defective in vulval epithelial invagination and have a severe reduction in hermaphrodite fertility. The gene sqv-7 encodes a multitransmembrane hydrophobic protein resembling nucleotide sugar transporters of the Golgi membrane. A Golgi vesicle enriched fraction of Saccharomyces c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berninsone P,Hwang HY,Zemtseva I,Horvitz HR,Hirschberg CB

更新日期：2001-03-27 00:00:00

abstract：:Interactions between swimming cells and surfaces are essential to many microbiological processes, from bacterial biofilm formation to human fertilization. However, despite their fundamental importance, relatively little is known about the physical mechanisms that govern the scattering of flagellated or ciliated cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kantsler V,Dunkel J,Polin M,Goldstein RE

更新日期：2013-01-22 00:00:00

abstract：:Leukotrienes (LTs) are signaling molecules derived from arachidonic acid that initiate and amplify innate and adaptive immunity. In turn, how their synthesis is organized on the nuclear envelope of myeloid cells in response to extracellular signals is not understood. We define the supramolecular architecture of LT syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandal AK,Jones PB,Bair AM,Christmas P,Miller D,Yamin TT,Wisniewski D,Menke J,Evans JF,Hyman BT,Bacskai B,Chen M,Lee DM,Nikolic B,Soberman RJ

更新日期：2008-12-23 00:00:00

abstract：:Multiple sclerosis is a chronic disease of the central nervous system (CNS) characterized by inflammation, demyelination, and axonal loss. The immunopathogenesis of demyelination in multiple sclerosis involves an autoantibody response to myelin oligodendrocyte glycoprotein (MOG), a type I transmembrane protein located...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breithaupt C,Schubart A,Zander H,Skerra A,Huber R,Linington C,Jacob U

更新日期：2003-08-05 00:00:00

abstract：:This perspective addresses recent advances in lipid transport across the Gram-negative inner and outer membranes. While we include a summary of previously existing literature regarding this topic, we focus on the maintenance of lipid asymmetry (Mla) pathway. Discovered in 2009 by the Silhavy group [J. C. Malinverni, T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Powers MJ,Trent MS

更新日期：2019-08-27 00:00:00

abstract：:To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Peca J,Matsuzaki M,Matsuzaki K,Noguchi J,Qiu L,Wang D,Zhang F,Boyden E,Deisseroth K,Kasai H,Hall WC,Feng G,Augustine GJ

更新日期：2007-05-08 00:00:00

abstract：:Gene amplification may be visualized within a chromosome as a homogeneously stained region (HSR) and HSRs have rarely been reported in human tumor cells with identification of the amplified gene. A parental line and seven clones derived from KB cells resistant to methotrexate (MTX) contain dihydrofolate reductase (DHF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolman SR,Craven ML,Grill SP,Domin BA,Cheng YC

更新日期：1983-02-01 00:00:00