Abstract：
:HaCaT keratinocytes differ from normal human epidermal keratinocytes (HEK) by constitutive expression of differentiation markers which are normally suppressed by vitamin A. In search of an explanation for this discrepancy we compared the vitamin A content, the expression of retinoid-binding proteins, and the vitamin A metabolism in the two cell types. The concentrations of retinol and 3,4-didehydroretinol in cultured HaCaT cells were less than one-fifth those in HEK, and the content of fatty acyl esters was even lower. Similarly, the concentrations of cellular retinol-binding protein and cellular retinoic acid-binding protein (CRBPI and CRABPII, respectively) were 10-30 times lower in HaCaT cells than in HEK corresponding to a reduced mRNA expression of these proteins. Unexpectedly, HaCaT cells expressed RARbeta in addition to RARalpha, RARgamma and RXRalpha, which are nuclear receptors normally found in HEK. Radioactive retinol added to the culture medium appeared only transiently in HaCaT cells, and pulse labeling confirmed a defective cellular retention of retinyl esters. After 24 h of incubation with [3H]retinol, cell-associated radioactivity corresponding to retinol, 3,4-didehydroretinol, all-trans-retinoic acid and 3,4-didehydroretinoic acid was found in both HaCaT cells and HEK. [3H]Retinoic acid showed a more rapid metabolism to 4-hydroxy/4-keto-retinoic acid in HaCaT cells than in HEK, which could be explained by a higher expression of cytochrome p450RAI in the former cells. In conclusion, the abnormal uptake of vitamin A and low levels of retinoid binding proteins in HaCaT cells, linked with an aberrant metabolism of retinol, may help to explain why these cells differentiate also in the presence of retinoids.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Törmä H,Rollman O,Vahlquist Adoi
10.1007/s004030050419keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
339-45issue
6eissn
0340-3696issn
1432-069Xjournal_volume
291pub_type
杂志文章abstract：:Comparative measurements of collagen synthesis as a portion of total protein synthesis were performed with fragments of peripheral neurofibromas, of skin adjacent to the tumors, and of unaffected skin from patients with neurofibromatosis (NF). No significant difference was found among these various samples. Collagen s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412930
更新日期：1986-01-01 00:00:00
abstract：:Conditions of oxidative stress lead to down-regulation of glutathione (GSH) and glutathione peroxidase (GPO), which could be responsible for tyrosinase induction in pigment cells. To address this question, the effects of selective modulation of GSH metabolism on melanogenic parameters of slightly and highly melanized ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050202
更新日期：1997-05-01 00:00:00
abstract：:The penetration through the epidermis in vitro of various topically used corticosteroids was compared. The amount penetrating through the epidermis from an ethanolic solution showed good correlation with the polarity of the corticosteroids under study. Hydrocortisone, the most polar corticosteroid, penetrates the epid...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412706
更新日期：1979-05-29 00:00:00
abstract：:The effect of the proteolytic enzyme savinase on human plantar skin in vitro was examined by light and electron microscopy to elucidate the morphological involvement of activated savinase in human epidermis. Light microscopic examination of incubated skin fragments demonstrated that the histological changes produced b...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371819
更新日期：1991-01-01 00:00:00
abstract：:Solar radiation in the UVB range is absorbed primarily by the epidermal DNA where characteristic photodamage results in altered immune responses and mutagenic lesions. UVB exposure of the skin results in a profound upregulation of the anti-inflammatory cytokine, IL-10 and suppression of contact hypersensitivity (CHS)....
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0634-0
更新日期：2006-03-01 00:00:00
abstract：:Human eccrine sweat glands were embedded in Lowicryl K4M. Cytokeratin proteins and blood group H antigen were localized by applying a postembedding immunogold method using a monoclonal antikeratin antibody and the lectin Ulex europaeus I. The antikeratin antibody labeled intermediate filaments in the secretory coil an...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00505639
更新日期：1990-01-01 00:00:00
abstract：:Congenital atrichia (AUC) is a form of isolated alopecia with an autosomal recessive mode of inheritance. Patients are born with normal hair but this is shed almost completely during the first weeks or months of life and never regrows. In many families the development of papular lesions is noted as an additional pheno...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-007-0747-8
更新日期：2007-06-01 00:00:00
abstract：:We investigated the effects of 1-4% ointments of metronidazole and tinidazole (derivatives of nitroimidazole) on models of inflammatory dermatitis evoked by antigen, hapten and monoclonal anti-dinitrophenol (DNP) IgE antibody in mice. Metronidazole and tinidazole ointments (1) suppressed the late-phase reaction (LPR) ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-002-0381-4
更新日期：2003-03-01 00:00:00
abstract：:Murine mast cell proliferation and maturation are regulated by two distinct cytokines, interleukin-3 (IL-3) and the c-kit ligand, stem cell factor (SCF). In this study using cells of the mouse mast cell line, MC/9, the effects of two immunosuppressants, FK506 and cyclosporin A (CsA), were investigated. Withdrawal of I...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050408
更新日期：1999-05-01 00:00:00
abstract：:In 8 patients with idiopathic chronic urticaria suction blisters were produced simultaneously on lesional and perilesional skin in the periumbilical area without application of heat. The suction pressure was 250 mm Hg, and thus the blister production lasted from 60--120 min. The blister fluid histamine was determined ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00418572
更新日期：1979-11-01 00:00:00
abstract：:Metalloproteinase-mediated proteolysis plays an important role during all stages of wound repair. In acute murine wounds, murine collagenase, matrix metalloproteinase (MMP)-9, stromelysin-1, stromelysin-2 and tissue inhibitor of metalloproteinase (TIMP)-1 are strongly induced within 24 h after wounding. At the time of...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/pl00007450
更新日期：1998-07-01 00:00:00
abstract：:Little attention has been given to the involvement of sweat glands/ducts in the pathogenesis of prurigo nodularis (PN). According to recent studies, PN is likely to develop under conditions characterized by dry skin, such as atopic dermatitis (AD), suggesting a strong impact of skin dryness on PN development. No thera...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01937-6
更新日期：2019-09-01 00:00:00
abstract：:In order to find, whether 8-MOP has an inhibiting effect on the proliferation of lymphocytes in the dark, we studied in vitro the lymphocyte proliferation by a mitogen (HgCl2) and by an antigen (tuberculin) from 16 psoriatic patients during the first oral PUVA treatment immediately before 8-MOP intake (03.--0.5 mg/kg ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00455295
更新日期：1978-05-31 00:00:00
abstract：:It has been reported that artificial restoration of barrier function by a water vapor-impermeable membrane after tape stripping induces barrier abrogation in hairless mice, impeding rather than enhancing barrier recovery. To address this issue, we examined the morphologic changes in the epidermis after tape stripping ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050280
更新日期：1998-03-01 00:00:00
abstract：:Besides its role in small-cell carcinoma of the lung, elevated serum levels of neuron-specific enolase (NSE) have recently been reported to be associated with autoimmune rheumatic disorders such as systemic sclerosis. Serum NSE seems to correlate with disease activity as well as Rodnan skin score. The aim of the study...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-004-0495-y
更新日期：2004-09-01 00:00:00
abstract：:Exposure in utero has been suggested to influence health later in life. The aim of this study was to investigate, if the use of prenatal food supplements was associated with atopic dermatitis in the offspring. Mothers who gave birth in the hospital G. B. Grassi were invited to participate in the study (n = 395). Infor...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01911-2
更新日期：2019-07-01 00:00:00
abstract：:Recently, medium-dose UVA1 phototherapy (50 J/cm2) has been introduced as an effective treatment for severe atopic dermatitis (AD). In order to further elucidate the mechanisms by which medium-dose UVA1 irradiation leads to an improvement in skin status in patients with AD, biopsy specimens from ten patients before an...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030100216
更新日期：2001-04-01 00:00:00
abstract：:The present study aimed to observe the therapeutic effect of combined mizolastine and proteoglycan in chronic urticaria. The patients were randomly divided into the treatment group (n = 56) and the control group (n = 44). The treatment group was medicated with calcium gluconate (10 mg/ time, 1 time/day), vitamin D3 (i...
journal_title：Archives of dermatological research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00403-019-01967-0
更新日期：2019-12-01 00:00:00
abstract：:The fetal development of Merkel cells-neuroendocrine cells of the skin - has been a matter of debate for a long time. Recent results have helped to confirm their intraepidermal development in humans. Simple epithelial cytokeratins (CK) nos. 8, 18, 19 and 20 are well established markers at the light microscopic level. ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505222
更新日期：1996-04-01 00:00:00
abstract：:Electron microscopic aspects in ten cases of normolipidemic cutaneous xanthomatosis have been investigated. Two additional types IV and V hyperlipoproteinaemic xanthomatosis have also been included. Ultrastructural findings in all cases were similar. Abundant histiocytic cells with numerous intracytoplasmic lipid vacu...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00694624
更新日期：1979-10-01 00:00:00
abstract：:In many skin diseases such as Demodex folliculitis, rosacea- or steroid-induced rosacea Demodex mites are present in abundance and are at least partially held responsible for causing these disorders. Although it is known that these diseases respond well to tetracyclines, it is unclear if this is due to the antiinflamm...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0984-0
更新日期：2009-10-01 00:00:00
abstract：:The cellular origin of Merkel cell carcinoma (MCC) is controversial. We previously hypothesized that MCC originates from hair follicle stem cells or Merkel cell (MC) progenitors residing within the hair follicle bulge. Examination of three cases of combined MCC led to the unexpected discovery that numerous keratin 20 ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-018-1877-x
更新日期：2019-01-01 00:00:00
abstract：:Lichen planus (LP) is a chronic inflammatory, T cell-mediated autoimmune skin disease. Innate immunity could explain the interplay between environmental triggers and the autoimmune cascade leading to disease development. Toll-like receptors (TLRs) are important components of the innate immune system, with no previous ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-012-1267-8
更新日期：2013-03-01 00:00:00
abstract：:Tumor-associated antigens were demonstrated in phenol water extracts of human malignant melanoma by migration tests with leukocytes from melanoma patients and controls: in 25 out of 60 melanoma patients weak reactivity was observed, usually stimulation of leukocyte migration, while leukocytes from 37 controls were neg...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417277
更新日期：1979-02-23 00:00:00
abstract：:Mite antigens play important roles in the pathogenesis of atopic dermatitis (AD). We recently developed a novel air cleaner (KC-850U) using charged plasma cluster ions to eliminate a variety of allergens from house environments. The present work demonstrates the ability of KC-850U to decrease the symptoms of AD induce...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1095-7
更新日期：2011-07-01 00:00:00
abstract：:Programmed cell death protein-1 (PD-1) is primarily recognized as an inhibitory receptor involved in the regulation of immunological tolerance. However, recent studies have indicated that PD-1/PD-L1 signaling could also regulate the functions of nonimmune cells and may be involved in regulating hair biology. In this s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02169-9
更新日期：2021-01-05 00:00:00
abstract：:Recently, we could show that the expression levels of the key regulators of the microRNA (miRNA) maturation and transport were dysregulated in inflamed hidradenitis suppurativa (HS) tissue (Heyam et al. in Wiley Interdiscip Rev RNA 6:271-289, 2015). The RNA-induced silencing complex (RISC) is the central element of th...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1752-1
更新日期：2017-09-01 00:00:00
abstract：:In a previous study, impedance measurements and phoreographic response were shown to quantify significant differences between involved and non-involved skin in psoriasis. The same techniques were used to objectivate the evolution on skin condition on patients treated with dioxyanthranol, difluprednate, and photochemot...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00431132
更新日期：1979-03-31 00:00:00
abstract：:A 61-year old woman developed a tumourous nodule in her left leg. Fifteen years ago a similar tumour had been removed in the same localization. Histological examination performed on biopsy material showed bundles of predominantly fusiform tumour cells with nuclear pleomorphism and many mitotic figures. Electron micros...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00446437
更新日期：1978-11-10 00:00:00
abstract：:An aromatic retinoid (Ro 10-9359) in 13-cis retinoic acid (Ro 4-3780) were fed by gastric tube to 170 male adult Syrian hamsters daily for 30 days. A low dose of 1 or 2 mg/kg and a high dose of 20 mg/kg body weight were selected. The effects of both retinoids on the sebaceous glands were assessed planimetrically (15 g...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00404284
更新日期：1980-01-01 00:00:00
