Abstract:
:The SMK1 mitogen-activated protein kinase is required for spore morphogenesis in Saccharomyces cerevisiae. In contrast to the multiple aberrant spore wall assembly patterns seen even within a single smk1 null ascus, different smk1 missense mutants block in a coordinated fashion at intermediate stages. One smk1 mutant forms asci in which the four spores are surrounded only by prospore wall-like structures, while another smk1 mutant forms asci in which the spores are surrounded by inner but not outer spore wall layers. Stepwise increases in gene dosage of a hypomorphic smk1 allele allow for the completion of progressively later morphological and biochemical events and for the acquisition of distinct spore-resistance phenotypes. Furthermore, smk1 allelic spore phenotypes can be recapitulated by reducing wild-type SMK1 expression. The data demonstrate that SMK1 is required for the execution of multiple steps in spore morphogenesis that require increasing thresholds of SMK1 activity. These results suggest that quantitative changes in mitogen-activated protein kinase signaling play a role in coordinating multiple events of a single cellular differentiation program.
journal_name
Geneticsjournal_title
Geneticsauthors
Wagner M,Briza P,Pierce M,Winter Ekeywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
1327-40issue
4eissn
0016-6731issn
1943-2631journal_volume
151pub_type
杂志文章相关文献
GENETICS文献大全abstract::Meiosis was analyzed cytogenetically in autotetraploids of Arabidopsis, including both established lines and newly generated autotetraploid plants. Fluorescent in situ hybridization with 5S and 45S rDNA probes was used to identify the different chromosomes at metaphase I of meiosis. Multivalents were observed frequent...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::The ability of autonomously replicating plasmids to recombine in mammalian cells was investigated. Two deletion plasmids of the eukaryotic-prokaryotic shuttle vector pSV2neo were cotransfected into transformed monkey COS cells. Examination of the low molecular weight DNA isolated after 48 hr of incubation revealed tha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Ribosome biogenesis is tightly regulated through stress-sensing pathways that impact genome stability, aging and senescence. In Saccharomyces cerevisiae, ribosomal RNAs are transcribed from rDNA located on the right arm of chromosome XII. Numerous studies reveal that rDNA decondenses into a puff-like structure during ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302994
更新日期:2020-03-01 00:00:00
abstract::The past century has seen substantial theoretical and empirical progress on the genetic basis of adaptation. Over this same period, a pressing need to prevent the evolution of drug resistance has uncovered much about the potential genetic basis of persistence in declining populations. However, we have little theory to...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302890
更新日期:2020-02-01 00:00:00
abstract::Boundaries (insulators) in the Drosophila bithorax complex (BX-C) delimit autonomous regulatory domains that orchestrate the parasegment (PS)-specific expression of the BX-C homeotic genes. The Fab-7 boundary separates the iab-6 and iab-7 regulatory domains, which control Abd-B expression in PS11 and PS12, respectivel...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301259
更新日期:2018-10-01 00:00:00
abstract::In this article, we utilize stochastic search variable selection methodology to develop a Bayesian method for identifying multiple quantitative trait loci (QTL) for complex traits in experimental designs. The proposed procedure entails embedding multiple regression in a hierarchical normal mixture model, where latent ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Our previous work suggests that the Nhp6 HMGB protein stimulates RNA polymerase II transcription via the TATA-binding protein TBP and that Nhp6 functions in the same functional pathway as the Gcn5 histone acetyltransferase. In this report we examine the genetic relationship between Nhp6 and Gcn5 with the Mot1 and Ccr4...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.050245
更新日期:2006-02-01 00:00:00
abstract::Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.106203
更新日期:2009-09-01 00:00:00
abstract::HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Expectations for the time scale and structure of allelic genealogies in finite populations are formed under three models of sporophytic self-incompatibility. The models differ in the dominance interactions among the alleles that determine the self-incompatibility phenotype: In the SSIcod model, alleles act codominantl...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Hepatic glucocorticoid receptor binding activity was measured in A/J, C57BL/6J, their F1 reciprocal crosses and their F1 recombinant inbred (RI) lines. The glucocorticoid binding capacity was measured in Hepes buffer and Hepes buffer plus dithiothreitol (DTT). The A/J parental strain showed higher levels, and a greate...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::The RNA polymerase II (Pol-II) holoenzyme, responsible for messenger RNA production, typically consists of 10-12 subunits. Our laboratory previously demonstrated that maternally deposited, long, noncoding, template RNAs are essential for programmed genome rearrangements in the ciliate Oxytricha trifallax. Here we show...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163279
更新日期:2014-07-01 00:00:00
abstract::Homolog recombination and unequal sister chromatid recombination were monitored in rad1-1/rad1-1 diploid yeast cells deficient for excision repair, and in control cells, RAD1/rad1-1, after exposure to UV irradiation. In a rad1-1/rad1-1 diploid, UV irradiation stimulated much more sister chromatid recombination relativ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::The larval cuticle protein (Lcp) cluster in Drosophila melanogaster contains four functional genes and a closely related pseudogene. A 630-bp fragment including the larval cuticle pseudogene locus (Lcp psi) was nucleotide sequenced in 10 strains of D. melanogaster and a 458-bp Lcp psi fragment from D. simulans was als...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::COTRANS is a program for analyzing cotransduction data. It calculates distances from pairwise cotransduction frequencies, computes crossovers required to obtain each observed recombinant class, and applies rules to draw conclusions about order. The rules are based on the correlation between the frequency of the classe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Diversity in insect pigmentation, encompassing a wide range of colors and spatial patterns, is among the most noticeable features distinguishing species, individuals, and body regions within individuals. In holometabolous species, a significant portion of such diversity can be attributed to the melanin synthesis genes...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.186684
更新日期:2016-05-01 00:00:00
abstract::In some strains of Saccharomyces cerevisiae, the induction of enzymes of the Leloir pathway, galactose fermentation and growth on galactose depend on mitochondrial function; mitochondrial dependence is elicited through the recessive allele imp1 of the nuclear gene IMP1. The genetic element IMP1 is not allelic to any o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Retroviruses and their relatives, the long terminal repeat (LTR) retrotransposons, carry out complex life cycles within the cells of their hosts. We have exploited a collection of gene deletion mutants developed by the Saccharomyces Genome Deletion Project to perform a functional genomics screen for host factors that ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::In most eukaryotes, crossovers are not independently distributed along the length of a chromosome. Instead, they appear to avoid close proximity to one another--a phenomenon known as crossover interference. Previously, for three of the five Arabidopsis chromosomes, we measured the strength of interference and suggeste...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.040055
更新日期:2005-06-01 00:00:00
abstract::Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026617
更新日期:2004-09-01 00:00:00
abstract::Evolution through natural selection suggests unnecessary genes are lost. We observed that the yeast Candida glabrata lost the gene encoding a phosphate-repressible acid phosphatase (PHO5) present in many yeasts including Saccharomyces cerevisiae. However, C. glabrata still had phosphate starvation-inducible phosphatas...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.120824
更新日期:2010-11-01 00:00:00
abstract::In its hypermutable state, an unstable singed allele, sn(w), mutates in the germline to two other alleleic forms at a total frequency usually between 40 and 60%. In its stable state, the mutation rate of sn(w) is essentially zero. Its state depends on an extrachromosomal condition indistinguishable from a property cal...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::Neural ganglia of wild type third-instar larvae of Drosophila melanogaster were incubated for 13 hours at various concentrations of BUdR (1, 3, 9, 27 micrograms/ml). Metaphases were collected with colchicine, stained with Hoechst 33258, and scored under a fluorescence microscope. Metaphases in which the sister chromat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::Individual inbreeding and historical demography can be estimated by analyzing runs of homozygosity (ROH), which are indicative of chromosomal segments of identity by descent (IBD). Such analyses have so far been rare in natural populations due to limited genomic resources. We analyzed ROH in whole genome sequences fro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198861
更新日期:2017-03-01 00:00:00
abstract::Drosophila adult leg development provides an ideal model system for characterizing the molecular mechanisms of hormone-triggered morphogenesis. A pulse of the steroid hormone ecdysone at the onset of metamorphosis triggers the rapid transformation of a flat leg imaginal disc into an immature adult leg, largely through...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::We identified the Drosophila melanogaster Signal peptide peptidase gene (Spp) that encodes a multipass transmembrane aspartyl protease. Drosophila SPP is homologous to the human signal peptide peptidase (SPP) and is distantly related to the presenilins. We show that, like human SPP, Drosophila SPP can proteolyze a mod...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.039933
更新日期:2005-05-01 00:00:00
abstract::Previous studies on gene expression in aneuploids revealed numerous trans-acting dosage effects. Segmental aneuploidy of each varied chromosomal region exhibited predominantly inverse effects on several target genes. Here, dosage regulation was examined in a maize (Zea mays L.) ploidy series where the complete genomic...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::Yeast mitochondria form a branched tubular network. Mitochondrial inheritance is tightly coupled with bud emergence, ensuring that daughter cells receive mitochondria from mother cells during division. Proteins reported to influence mitochondrial inheritance include the mitochondrial rho (Miro) GTPase Gem1p, Mmr1p, an...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083055
更新日期:2008-02-01 00:00:00
abstract::We have analyzed donor and target sites of the mobile element Activator (Ac) that are altered as a result of somatic transposition from the P locus in maize. Previous genetic analysis has indicated that the two mitotic daughter lineages which result from Ac transposition from P differ in their Ac constitution at the P...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00
abstract::Two strains of Drosophila melanogaster derived from different geographical localities were subjected to selection for fast and slow developmental rate. Four enzyme polymorphisms were monitored for their response to such selection. Significant changes in allele frequencies were effected for the alphaGpdh and 6Pgd polym...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00