The BEN Domain Protein Insensitive Binds to the Fab-7 Chromatin Boundary To Establish Proper Segmental Identity in Drosophila.

Abstract:

:Boundaries (insulators) in the Drosophila bithorax complex (BX-C) delimit autonomous regulatory domains that orchestrate the parasegment (PS)-specific expression of the BX-C homeotic genes. The Fab-7 boundary separates the iab-6 and iab-7 regulatory domains, which control Abd-B expression in PS11 and PS12, respectively. This boundary is composed of multiple functionally redundant elements and has two key functions: it blocks cross talk between iab-6 and iab-7 and facilitates boundary bypass. Here, we show that two BEN domain protein complexes, Insensitive and Elba, bind to multiple sequences located in the Fab-7 nuclease hypersensitive regions. Two of these sequences are recognized by both Insv and Elba and correspond to a CCAATTGG palindrome. Elba also binds to a related CCAATAAG sequence, while Insv does not. However, the third Insv recognition sequences is ∼100 bp in length and contains the CCAATAAG sequence at one end. Both Insv and Elba are assembled into large complexes (∼420 and ∼265-290 kDa, respectively) in nuclear extracts. Using a sensitized genetic background, we show that the Insv protein is required for Fab-7 boundary function and that PS11 identity is not properly established in insv mutants. This is the first demonstration that a BEN domain protein is important for the functioning of an endogenous fly boundary.

journal_name

Genetics

journal_title

Genetics

authors

Fedotova A,Aoki T,Rossier M,Mishra RK,Clendinen C,Kyrchanova O,Wolle D,Bonchuk A,Maeda RK,Mutero A,Cleard F,Mogila V,Karch F,Georgiev P,Schedl P

doi

10.1534/genetics.118.301259

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

573-585

issue

2

eissn

0016-6731

issn

1943-2631

pii

genetics.118.301259

journal_volume

210

pub_type

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