当前位置： SCI文献检索 > Translational Oncology期刊下所有文献 > Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer.

Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer.

Abstract：

OBJECTIVE:This study investigated the feasibility of using malignant pleural effusion (MPE) supernatant and paired cell blocks (precipitate) for gene profiling in patients with non-small cell lung cancer (NSCLC) using next-generation sequencing (NGS) technique. METHODS:Stage IV non-squamous NSCLC patients with MPE were eligible in this prospective study and recruited from Zhejiang Cancer Hospital between May 2014 and October 2015. MPE supernatant and paired precipitate sample gene alterations were determined with NGS containing 14 cancer-related genes. Progression free survival (PFS) was evaluated using Kaplan-Meier method and compared using log-rank test. RESULTS:A total of 102 patients were enrolled in the present study. All pleural effusions were confirmed as malignant with cytological smears. A total of 77 paired MPE supernatant and precipitate samples were acquired from the 102 patients. The results revealed that there were no statistically significant differences in the detection rate and maximum allelic fraction between supernatant and precipitate samples (P = 1.0 and P = .6). Collectively, 172 and 158 genomic alterations with 112 shared mutations were identified in supernatant and precipitate samples, respectively. Comparable PFS was found in EGFR mutation patients according to the supernatant and precipitate sample results (14.0 vs.13.9 months, P = .90). CONCLUSIONS:These results demonstrated that MPE supernatants were comparable to precipitate samples for detection of genetic alterations. However, gene mutation heterogeneity was found between these two media types.

journal_name

Transl Oncol

journal_title

Translational oncology

authors

Li J,Li X,Wang W,Shao Y,Zhang Y,Song Z

doi

10.1016/j.tranon.2020.100784

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

100784

issue

8

issn

1936-5233

pii

S1936-5233(20)30071-1

journal_volume

13

pub_type

杂志文章

相关文献

Translational Oncology文献大全
  • Down-regulation of miR-21 Induces Differentiation of Chemoresistant Colon Cancer Cells and Enhances Susceptibility to Therapeutic Regimens.

    abstract：:MicroRNAs are endogenous posttranscriptional modulators that negatively control the expression of their target genes and play an important role in the development and progression of many malignancies, including colorectal carcinoma. In particular, expression of microRNA-21 (miR-21) is greatly increased in chemotherapy...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.12397

    authors： Yu Y,Sarkar FH,Majumdar AP

    更新日期：2013-04-01 00:00:00

  • Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer.

    abstract：:The purpose of this study was to investigate the usefulness of photoacoustic imaging (PAI) for spatiotemporal mapping of tumor hemodynamics in a rabbit model of head and neck carcinoma. Shope cottontail rabbit papilloma virus associated VX2 carcinomas were established in adult male New Zealand White rabbits (n = 9) by...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2017.08.004

    authors： Rich LJ,Sexton S,Curtin L,Seshadri M

    更新日期：2017-10-01 00:00:00

  • Inhibitor of DNA Binding 2 Inhibits Epithelial-Mesenchymal Transition via Up-Regulation of Notch3 in Breast Cancer.

    abstract：:Breast cancer is the second leading cause of cancer death in women worldwide. Incurable metastatic breast disease presents a major clinical challenge and is the main cause of breast cancer-related death. The epithelial-mesenchymal transition (EMT) is a critical early promoter of metastasis. In the present study, we id...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2018.07.015

    authors： Wen XF,Chen M,Wu Y,Chen MN,Glogowska A,Klonisch T,Zhang GJ

    更新日期：2018-10-01 00:00:00

  • Circulating Exosomal miR-17-5p and miR-92a-3p Predict Pathologic Stage and Grade of Colorectal Cancer.

    abstract：:Exosomes are extracellular membrane vesicles of 50- to 130-nm diameter secreted by most tumor cells. Exosomes can mediate the intercellular transfer of proteins and RNAs, including microRNAs (miRNAs), and promote both tumorigenesis and premetastatic niche formation. In this study, we performed exosomal RNA sequencing ...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2017.12.012

    authors： Fu F,Jiang W,Zhou L,Chen Z

    更新日期：2018-04-01 00:00:00

  • Altered microRNA Expression Patterns in Hepatoblastoma Patients.

    abstract：:Liver cancers in children are rare representing only 1.1% of malignancies, with an annual incidence rate of 1.5 cases per million. Hepatoblastoma and hepatocellular carcinomas are the most common malignancies of the liver occurring in young people aged 15 years or younger. Molecular basis of both tumors are still uncl...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.09124

    authors： Magrelli A,Azzalin G,Salvatore M,Viganotti M,Tosto F,Colombo T,Devito R,Di Masi A,Antoccia A,Lorenzetti S,Maranghi F,Mantovani A,Tanzarella C,Macino G,Taruscio D

    更新日期：2009-08-18 00:00:00

  • hERG1 and HIF-2α Behave as Biomarkers of Positive Response to Bevacizumab in Metastatic Colorectal Cancer Patients.

    abstract：BACKGROUND:In search of novel biomarkers of response to bevacizumab in metastatic colorectal cancer (mCRC), we analyzed the expression and prognostic role of several proteins related to angiogenesis. METHODS:A retrospective, multicenter study on 80 surgical samples from mCRC patients treated in first line with bevaciz...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.01.001

    authors： Iorio J,Lastraioli E,Tofani L,Petroni G,Antonuzzo L,Messerini L,Perrone G,Caputo D,Francesconi M,Amato MM,Cadei M,Arcangeli G,Villanacci V,Boni L,Coppola R,Di Costanzo F,Arcangeli A

    更新日期：2020-03-01 00:00:00

  • Wnt Signaling Drives Prostate Cancer Bone Metastatic Tropism and Invasion.

    abstract：:Wnt signaling has been implicated as a driver of prostate cancer-related osteoblast differentiation, and previous studies have linked modifications in Wnt function with the induction of tumor metastasis. A unique aspect of prostate cancer bone metastases in mouse models is their relative predilection to the hindlimb (...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100747

    authors： Wang Y,Singhal U,Qiao Y,Kasputis T,Chung JS,Zhao H,Chammaa F,Belardo JA,Roth TM,Zhang H,Zaslavsky AB,Palapattu GS,Pienta KJ,Chinnaiyan AM,Taichman RS,Cackowski FC,Morgan TM

    更新日期：2020-04-01 00:00:00

  • Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas.

    abstract：:BNIP3 (Bcl-2/adenovirus E1B 19-kDa interacting protein 3) is a BH3-only protein that regulates apoptosis and autophagy. BNIP3 plays also an important role in hypoxia-induced cell response and is regulated by HIF1. Here, we studied a possible association of BNIP3 expression and the prognosis of patients with renal cell...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2017.08.008

    authors： Macher-Goeppinger S,Keith M,Hatiboglu G,Hohenfellner M,Schirmacher P,Roth W,Tagscherer KE

    更新日期：2017-12-01 00:00:00

  • Ultrasound Guided Optoacoustic Tomography in Assessment of Tumor Margins for Lumpectomies.

    abstract：PURPOSE:To determine the accuracy of a handheld ultrasound-guided optoacoustic tomography (US-OT) probe developed for human deep-tissue imaging in ex vivo assessment of tumor margins postlumpectomy. METHODS:A custom-built two-dimensional (2D) US-OT-handheld probe was used to scan 15 lumpectomy breast specimens. Optoac...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2019.11.005

    authors： Goh Y,Balasundaram G,Moothanchery M,Attia A,Li X,Lim HQ,Burton NC,Qiu Y,Putti TC,Chan CW,Iau P,Buhari SA,Hartman M,Tang SW,Ng CWQ,Chan YH,Pool FJ,Pillay P,Chua W,Kapur J,Jagmohan P,Sterling E,Quek ST,Olivo M

    更新日期：2020-02-01 00:00:00

  • Immunotherapy with Dendritic Cells Modified with Tumor-Associated Antigen Gene Demonstrates Enhanced Antitumor Effect Against Lung Cancer.

    abstract：BACKGROUND:Immunotherapy using dendritic cell (DC) vaccine has the potential to overcome the bottleneck of cancer therapy. METHODS:We engineered Lewis lung cancer cells (LLCs) and bone marrow-derived DCs to express tumor-associated antigen (TAA) ovalbumin (OVA) via lentiviral vector plasmid encoding OVA gene. We then ...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2016.12.002

    authors： Jiang T,Chen X,Zhou W,Fan G,Zhao P,Ren S,Zhou C,Zhang J

    更新日期：2017-04-01 00:00:00

  • Prediction of cancer-specific survival and overall survival in middle-aged and older patients with rectal adenocarcinoma using a nomogram model.

    abstract：OBJECTIVE:To develop a new nomogram tool for predicting survival in middle-aged and elderly patients with rectal adenocarcinoma. METHODS:A total of 6,116 patients were randomly assigned in a 7:3 ratio to training and validation cohorts. Univariate and multivariate Cox proportional hazards regression analyses were used...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100938

    authors： Liu H,Lv L,Qu Y,Zheng Z,Zhao J,Liu B,Zhang D,Wang H,Zhang J

    更新日期：2021-01-01 00:00:00

  • The Rac Inhibitor EHop-016 Inhibits Mammary Tumor Growth and Metastasis in a Nude Mouse Model.

    abstract：:Metastatic disease still lacks effective treatments, and remains the primary cause of cancer mortality. Therefore, there is a critical need to develop better strategies to inhibit metastatic cancer. The Rho family GTPase Rac is an ideal target for anti-metastatic cancer therapy, because Rac is a key molecular switch t...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2014.07.004

    authors： Castillo-Pichardo L,Humphries-Bickley T,De La Parra C,Forestier-Roman I,Martinez-Ferrer M,Hernandez E,Vlaar C,Ferrer-Acosta Y,Washington AV,Cubano LA,Rodriguez-Orengo J,Dharmawardhane S

    更新日期：2014-10-24 00:00:00

  • Reproducibility and Prognosis of Quantitative Features Extracted from CT Images.

    abstract：:We study the reproducibility of quantitative imaging features that are used to describe tumor shape, size, and texture from computed tomography (CT) scans of non-small cell lung cancer (NSCLC). CT images are dependent on various scanning factors. We focus on characterizing image features that are reproducible in the p...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.13844

    authors： Balagurunathan Y,Gu Y,Wang H,Kumar V,Grove O,Hawkins S,Kim J,Goldgof DB,Hall LO,Gatenby RA,Gillies RJ

    更新日期：2014-02-01 00:00:00

  • Genetically Modified DR5-Specific TRAIL Variant DR5-B Revealed Dual Antitumor and Protumoral Effect in Colon Cancer Xenografts and an Improved Pharmacokinetic Profile.

    abstract：:Despite the weak clinical efficacy of TRAIL death receptor agonists, a search is under way for new agents that more efficiently activate apoptotic signaling. We previously created a TRAIL DR5-selective variant DR5-B without affinity for the DR4, DcR1, DcR2, and OPG receptors and increased proapoptotic activity in tumo...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100762

    authors： Yagolovich AV,Artykov AA,Karmakova TA,Vorontsova MS,Pankratov AA,Andreev-Andrievsky AA,Dolgikh DA,Kirpichnikov MP,Gasparian ME

    更新日期：2020-04-01 00:00:00

  • Angiogenesis and Antiangiogenesis in Triple-Negative Breast cancer.

    abstract：:Several data support a central role for angiogenesis in breast cancer growth and metastasis. Observational studies have demonstrated that microvascular density (MVD) is a prognostic factor in invasive breast cancer, whereas others reached the opposite conclusion. Vascular endothelial growth factor is the most importan...

    journal_title：Translational oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.tranon.2016.07.002

    authors： Ribatti D,Nico B,Ruggieri S,Tamma R,Simone G,Mangia A

    更新日期：2016-10-01 00:00:00

  • DPS-2: A Novel Dual MEK/ERK and PI3K/AKT Pathway Inhibitor with Powerful Ex Vivo and In Vivo Anticancer Properties.

    abstract：:Development of novel bioactive compounds against KRAS and/or BRAF mutant colorectal cancer (CRC) is currently an urgent need in oncology. In addition, single or multitarget kinase inhibitors against MEK/ERK and PI3K/AKT pathways are of potential therapeutic advantage. A new compound based on the benzothiophene nucleus...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2019.04.005

    authors： Goulielmaki M,Assimomytis N,Rozanc J,Taki E,Christodoulou I,Alexopoulos LG,Zoumpourlis V,Pintzas A,Papahatjis D

    更新日期：2019-07-01 00:00:00

  • Mutations, Differential Gene Expression, and Chimeric Transcripts in Esophageal Squamous Cell Carcinoma Show High Heterogeneity.

    abstract：:Esophageal squamous cell carcinoma (ESCC) is a frequent and lethal neoplasia. As recent advances in targeted therapy have not improved ESCC prognosis, characterization of molecular alterations associated to this tumor is of foremost relevance. In this study, we analyze, for the first time, the complete genomic profile...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2018.08.002

    authors： Souza-Santos PT,Soares Lima SC,Nicolau-Neto P,Boroni M,Meireles Da Costa N,Brewer L,Menezes AN,Furtado C,Moreira MAM,Seuanez HN,de Almeida Simão T,Ribeiro Pinto LF

    更新日期：2018-12-01 00:00:00

  • Nomogram based on preoperative CT imaging predicts the EGFR mutation status in lung adenocarcinoma.

    abstract：:Tyrosine kinase inhibitors (TKIs) provide clinical benefits to the lung cancer patients with epidermal growth factor receptor (EGFR) mutations. However, non-invasively determine EGFR mutation status in patients before targeted therapy remains a challenge. This study aimed to develop and validate a nomogram for preoper...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100954

    authors： Zhang G,Zhang J,Cao Y,Zhao Z,Li S,Deng L,Zhou J

    更新日期：2021-01-01 00:00:00

  • Strategies for high-resolution imaging of epithelial ovarian cancer by laparoscopic nonlinear microscopy.

    abstract：:Ovarian cancer remains the most frequently lethal of the gynecologic cancers owing to the late detection of this disease. Here, by using human specimens and three mouse models of ovarian cancer, we tested the feasibility of nonlinear imaging approaches, the multiphoton microscopy (MPM) and second harmonic generation (...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.09310

    authors： Williams RM,Flesken-Nikitin A,Ellenson LH,Connolly DC,Hamilton TC,Nikitin AY,Zipfel WR

    更新日期：2010-06-01 00:00:00

  • Heterogeneous Drug Efficacy of an Antibody-Drug Conjugate Visualized Using Simultaneous Imaging of Its Delivery and Intracellular Damage in Living Tumor Tissues.

    abstract：:Anticancer drug efficacy varies because the delivery of drugs within tumors and tumor responses are heterogeneous; however, these features are often more homogenous in vitro. This difference makes it difficult to accurately determine drug efficacy. Therefore, it is important to use living tumor tissues in preclinical ...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100764

    authors： Gonda K,Negishi H,Takano-Kasuya M,Kitamura N,Furusawa N,Nakano Y,Hamada Y,Tokunaga M,Higuchi H,Tada H,Ishida T

    更新日期：2020-06-01 00:00:00

  • Evaluation of potentially predictive markers for anti-angiogenic therapy with sunitinib in recurrent ovarian cancer patients.

    abstract：INTRODUCTION:In ovarian cancer, new therapeutic strategies are needed because the vast majority of patients develop a recurrence and resistance to platinum derivates. Attached to the AGO-OVAR2.11 study investigating the multityrosine kinase inhibitor sunitinib in recurrent platinum refractory ovarian cancers, this tran...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.13205

    authors： Bauerschlag DO,Hilpert F,Meier W,Rau J,Meinhold-Heerlein I,Maass N,Dubois A,Sehouli J,Arnold N,Schem C,Oberg HH,Baumann K

    更新日期：2013-06-01 00:00:00

  • DKK1 and Kremen Expression Predicts the Osteoblastic Response to Bone Metastasis.

    abstract：:Bone metastasis is a complication of advanced breast and prostate cancer. Tumor-secreted Dickkopf homolog 1 (DKK1), an inhibitor of canonical Wnt signaling and osteoblast differentiation, was proposed to regulate the osteoblastic response to metastatic cancer in bone. The objectives of this study were to compare DKK1 ...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2018.04.013

    authors： Clines KL,Clines GA

    更新日期：2018-08-01 00:00:00

  • CpG-coated prussian blue nanoparticles-based photothermal therapy combined with anti-CTLA-4 immune checkpoint blockade triggers a robust abscopal effect against neuroblastoma.

    abstract：:High-risk neuroblastoma, which is associated with regional and systemic metastasis, is a leading cause of cancer-related mortality in children. Responding to this need for novel therapies for high-risk patients, we have developed a "nanoimmunotherapy," which combines photothermal therapy (PTT) using CpG oligodeoxynucl...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100823

    authors： Cano-Mejia J,Shukla A,Ledezma DK,Palmer E,Villagra A,Fernandes R

    更新日期：2020-10-01 00:00:00

  • Three paths to better tyrosine kinase inhibition behind the blood-brain barrier in treating chronic myelogenous leukemia and glioblastoma with imatinib.

    abstract：:Chronic myelogenous leukemia (CML) can be controlled for years with the tyrosine kinase inhibitor imatinib but because imatinib poorly penetrates the blood-brain barrier (BBB), on occasion, the CML clone will thrive and evolve to an accelerated phase in the resulting imatinib sanctuary within the central nervous syste...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.09280

    authors： Kast RE,Focosi D

    更新日期：2010-02-01 00:00:00

  • Preoperative D-dimer and Gamma-Glutamyltranspeptidase Predict Major Complications and Survival in Colorectal Liver Metastases Patients After Resection.

    abstract：OBJECTIVES:To investigate the predictive value of the pre-operative D-dimer and gamma-glutamyltranspeptidase (GGT) for the prognosis in colorectal liver metastases (CRLM) patients after hepatic resection. METHODS:Two hundred and ninety-two patients between December 2008 and December 2016 and 101 patients at our center...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2019.04.011

    authors： Chen Q,Zhao H,Wu J,Cai J,Li C,Zhao J,Bi X,Li Z,Huang Z,Zhang Y,Cui W,Zhou J

    更新日期：2019-07-01 00:00:00

  • Therapeutic relevance of molecular screening program in patients with metastatic sarcoma: Analysis from the ProfiLER 01 trial.

    abstract：BACKGROUND:Advanced sarcoma is a group of heterogeneous disease with poor prognosis and poor efficacy of medical treatment. They represent a promising group of tumors to assess molecular-based therapy (MBT) strategy. PATIENTS AND METHODS:Genomic profiles of patients with advanced sarcoma included in the ProfiLER progr...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2020.100870

    authors： Arnaud-Coffin P,Brahmi M,Vanacker H,Eberst L,Tredan O,Attignon V,Pissaloux D,Sohier E,Cassier P,Garin G,Pérol D,Blay JY,Dufresne A

    更新日期：2020-12-01 00:00:00

  • The Potential Value of Preoperative MRI Texture and Shape Analysis in Grading Meningiomas: A Preliminary Investigation.

    abstract：OBJECT:Preoperative knowledge of meningioma grade is essential for planning treatment and surgery. The purpose of this study was to investigate the diagnostic value of MRI texture and shape analysis in grading meningiomas. METHODS:A surgical database was reviewed to identify meningioma patients who had undergone tumor...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2017.04.006

    authors： Yan PF,Yan L,Hu TT,Xiao DD,Zhang Z,Zhao HY,Feng J

    更新日期：2017-08-01 00:00:00

  • Selecting Potential Targetable Biomarkers for Imaging Purposes in Colorectal Cancer Using TArget Selection Criteria (TASC): A Novel Target Identification Tool.

    abstract：:Peritoneal carcinomatosis (PC) of colorectal origin is associated with a poor prognosis. However, cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy is available for a selected group of PC patients, which significantly increases overall survival rates up to 30%. As a consequence, there is su...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1593/tlo.10220

    authors： van Oosten M,Crane LM,Bart J,van Leeuwen FW,van Dam GM

    更新日期：2011-04-01 00:00:00

  • Prognosis of Glioblastoma With Oligodendroglioma Component is Associated With the IDH1 Mutation and MGMT Methylation Status.

    abstract：:Glioblastoma (GBM) with oligodendroglioma component (GBMO) is a newly described GBM subtype in the 2007 World Health Organization classification. However, its biological and genetic characteristics are largely unknown. We investigated the clinicopathological and molecular features of 34 GBMOs and compared the survival...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2014.10.002

    authors： Myung JK,Cho HJ,Kim H,Park CK,Lee SH,Choi SH,Park P,Yoon JM,Park SH

    更新日期：2014-12-01 00:00:00

  • Collaboration Between RSK-EphA2 and Gas6-Axl RTK Signaling in Arginine Starvation Response That Confers Resistance to EGFR Inhibitors.

    abstract：:Many human malignancies require extracellular arginine (Arg) for survival because the key enzyme for de novo Arg biosynthesis, argininosuccinate synthetase 1 (ASS1), is silenced. Recombinant arginine deiminase (ADI-PEG20), which digests extracellular Arg, has been in clinical trials for treating ASS1-negative tumors. ...

    journal_title：Translational oncology

    pub_type： 杂志文章

    doi：10.1016/j.tranon.2019.12.003

    authors： Kuo MT,Long Y,Tsai WB,Li YY,Chen HHW,Feun LG,Savaraj N

    更新日期：2020-02-01 00:00:00