The Gβ1 and Gβ3 Subunits Differentially Regulate Rat Vascular Kv7 Channels.

Abstract:

:Within the vasculature Kv7 channels are key regulators of basal tone and contribute to a variety of receptor mediated vasorelaxants. The Kv7.4 isoform, abundant within the vasculature, is key to these processes and was recently shown to have an obligatory requirement of G-protein βγ subunits for its voltage dependent activity. There is an increasing appreciation that with 5 Gβ subunits and 12 Gγ subunits described in mammalian cells that different Gβ x γ x combinations can confer selectivity in Gβγ effector stimulation. Therefore, we aimed to characterize the Gβ subunit(s) which basally regulate Kv7.4 channels and native vascular Kv7 channels. In Chinese Hamster Ovary cells overexpressing Kv7.4 and different Gβx subunits only Gβ1, Gβ3, and Gβ5 enhanced Kv7.4 currents, increasing the activation kinetics and negatively shifting the voltage dependence of activation. In isolated rat renal artery myocytes, proximity ligation assay detected an interaction of Kv7.4 with Gβ1 and Gβ3 subunits, but not other isoforms. Morpholino directed knockdown of Gβ1 in rat renal arteries did not alter Kv7 dependent currents but reduced Kv7.4 protein expression. Knockdown of Gβ3 in rat renal arteries resulted in decreased basal K+ currents which were not sensitive to pharmacological inhibition of Kv7 channels. These studies implicate the Gβ1 subunit in the synthesis or stability of Kv7.4 proteins, whilst revealing that the Gβ3 isoform is responsible for the basal activity of Kv7 channels in native rat renal myocytes. These findings demonstrate that different Gβ subunits have important individual roles in ion channel regulation.

journal_name

Front Physiol

journal_title

Frontiers in physiology

authors

Greenwood IA,Stott JB

doi

10.3389/fphys.2019.01573

subject

Has Abstract

pub_date

2020-01-14 00:00:00

pages

1573

issn

1664-042X

journal_volume

10

pub_type

杂志文章
  • Winged Pea Aphids Can Modify Phototaxis in Different Development Stages to Assist Their Host Distribution.

    abstract::The pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae), shows wing polyphenism (winged and wingless morphs) in its life cycle. The winged morph is adapted for dispersal; its two developmental adult stages (for dispersal and reproduction) are based on its breeding periods. The two morphs show different phot...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2016.00307

    authors: Zhang Y,Wang XX,Jing X,Tian HG,Liu TX

    更新日期:2016-08-02 00:00:00

  • Effects of Fungicide Propiconazole on the Yeast-Like Symbiotes in Brown Planthopper (BPH, Nilaparvata lugens Stål) and Its Role in Controlling BPH Infestation.

    abstract::Yeast-like symbiotes (YLS), harbored in the abdomen fat-body cells of the rice brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), are vital to the growth and reproduction of their host. It is feasible to manipulate BPH infestation on rice by inhibiting YLS using fungicide. In this study, the fu...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00089

    authors: Shentu X,Wang X,Xiao Y,Yu X

    更新日期:2019-02-11 00:00:00

  • A Chemosensory Protein BtabCSP11 Mediates Reproduction in Bemisia tabaci.

    abstract::The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 g...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00709

    authors: Zeng Y,Merchant A,Wu Q,Wang S,Kong L,Zhou X,Xie W,Zhang Y

    更新日期:2020-06-30 00:00:00

  • Adrenergic Signaling-Induced Ultrastructural Strengthening of Intercalated Discs via Plakoglobin Is Crucial for Positive Adhesiotropy in Murine Cardiomyocytes.

    abstract::Intercalated discs (ICDs), which connect adjacent cardiomyocytes, are composed of desmosomes, adherens junctions (AJs) and gap junctions (GJs). Previous data demonstrated that adrenergic signaling enhances cardiac myocyte cohesion, referred to as positive adhesiotropy, via PKA-mediated phosphorylation of plakoglobin (...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00430

    authors: Yeruva S,Kempf E,Egu DT,Flaswinkel H,Kugelmann D,Waschke J

    更新日期:2020-05-21 00:00:00

  • Calcium-permeable ion channels in control of autophagy and cancer.

    abstract::Autophagy, or cellular self-eating, is a tightly regulated cellular pathway the main purpose of which is lysosomal degradation and subsequent recycling of cytoplasmic material to maintain normal cellular homeostasis. Defects in autophagy are linked to a variety of pathological states, including cancer. Cancer is the d...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2013.00272

    authors: Kondratskyi A,Yassine M,Kondratska K,Skryma R,Slomianny C,Prevarskaya N

    更新日期:2013-10-02 00:00:00

  • On Publication Strategies. Another Advice to a Beginning Scientist.

    abstract::Productivity in science has increased and it is becoming more important for scientists to publish, to publish frequently, and to accumulate citations to their work. However, the peer review system may not only promote and advance but also hinder, prevent, or delay publication. In this personal perspective, confirmator...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.01073

    authors: Coronel R

    更新日期:2020-09-04 00:00:00

  • The Role of Carotid Sinus Nerve Input in the Hypoxic-Hypercapnic Ventilatory Response in Juvenile Rats.

    abstract::In juvenile rats, the carotid body (CB) is the primary sensor of oxygen (O2) and a secondary sensor of carbon dioxide (CO2) in the blood. The CB communicates to the respiratory pattern generator via the carotid sinus nerve, which terminates within the commissural nucleus tractus solitarius (cNTS). While this is not th...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.613786

    authors: Getsy PM,Coffee GA,Lewis SJ

    更新日期:2020-12-17 00:00:00

  • Identification of Autophagy-Related Gene 7 and Autophagic Cell Death in the Planarian Dugesia japonica.

    abstract::Planarians undergo continuous body size remodeling under starvation or during regeneration. This process likely involves autophagy and autophagic cell death, but this hypothesis is supported by few studies. To test this hypothesis, we cloned and characterized autophagy-related gene 7 (Atg7) from the planarian Dugesia ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01223

    authors: Ma K,Zhang Y,Song G,Wu M,Chen G

    更新日期:2018-09-04 00:00:00

  • Masticatory Dysfunction by Extensive Tooth Loss as a Risk Factor for Cognitive Deficit: A Systematic Review and Meta-Analysis.

    abstract::Background: An amount of cognition decline is normal with aging; however, intrinsic and extrinsic risk factors may exacerbate it, affecting social and occupational tasks. Masticatory dysfunction (MD), as a general term, refers to an impairment in the masticatory function triggered by a structural factor, such as tooth...

    journal_title:Frontiers in physiology

    pub_type:

    doi:10.3389/fphys.2019.00832

    authors: Alvarenga MOP,Ferreira RO,Magno MB,Fagundes NCF,Maia LC,Lima RR

    更新日期:2019-07-03 00:00:00

  • Long-Term Chronic Intermittent Hypobaric Hypoxia Induces Glucose Transporter (GLUT4) Translocation Through AMP-Activated Protein Kinase (AMPK) in the Soleus Muscle in Lean Rats.

    abstract::Background: In chronic hypoxia (CH) and short-term chronic intermittent hypoxia (CIH) exposure, glycemia and insulin levels decrease and insulin sensitivity increases, which can be explained by changes in glucose transport at skeletal muscles involving GLUT1, GLUT4, Akt, and AMPK, as well as GLUT4 translocation to cel...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00799

    authors: Siques P,Brito J,Flores K,Ordenes S,Arriaza K,Pena E,León-Velarde F,López de Pablo ÁL,Gonzalez MC,Arribas S

    更新日期:2018-06-28 00:00:00

  • Update on 3-iodothyronamine and its neurological and metabolic actions.

    abstract::3-iodothyronamine (T1AM) is an endogenous amine, that has been detected in many rodent tissues, and in human blood. It has been hypothesized to derive from thyroid hormone metabolism, but this hypothesis still requires validation. T1AM is not a ligand for nuclear thyroid hormone receptors, but stimulates with nanomola...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2014.00402

    authors: Zucchi R,Accorroni A,Chiellini G

    更新日期:2014-10-16 00:00:00

  • Modeling the Pro-inflammatory Tumor Microenvironment in Acute Lymphoblastic Leukemia Predicts a Breakdown of Hematopoietic-Mesenchymal Communication Networks.

    abstract::Lineage fate decisions of hematopoietic cells depend on intrinsic factors and extrinsic signals provided by the bone marrow microenvironment, where they reside. Abnormalities in composition and function of hematopoietic niches have been proposed as key contributors of acute lymphoblastic leukemia (ALL) progression. Ou...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2016.00349

    authors: Enciso J,Mayani H,Mendoza L,Pelayo R

    更新日期:2016-08-19 00:00:00

  • Generation and applications of human pluripotent stem cells induced into neural lineages and neural tissues.

    abstract::Human pluripotent stem cells (hPSCs) represent a new and exciting field in modern medicine, now the focus of many researchers and media outlets. The hype is well-earned because of the potential of stem cells to contribute to disease modeling, drug screening, and even therapeutic approaches. In this review, we focus fi...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00047

    authors: Martinez Y,Dubois-Dauphin M,Krause KH

    更新日期:2012-03-19 00:00:00

  • Cardiovascular and Metabolic Responses to the Ingestion of Caffeinated Herbal Tea: Drink It Hot or Cold?

    abstract::Aim: Tea is usually consumed at two temperatures (as hot tea or as iced tea). However, the importance of drink temperature on the cardiovascular system and on metabolism has not been thoroughly investigated. The purpose of this study was to compare the cardiovascular, metabolic and cutaneous responses to the ingestion...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00315

    authors: Maufrais C,Sarafian D,Dulloo A,Montani JP

    更新日期:2018-04-06 00:00:00

  • SARS-CoV-2 Mediated Endothelial Dysfunction: The Potential Role of Chronic Oxidative Stress.

    abstract::Endothelial cells have emerged as key players in SARS-CoV-2 infection and COVID-19 inflammatory pathologies. Dysfunctional endothelial cells can promote chronic inflammation and disease processes like thrombosis, atherosclerosis, and lung injury. In endothelial cells, mitochondria regulate these inflammatory pathways ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2020.605908

    authors: Chang R,Mamun A,Dominic A,Le NT

    更新日期:2021-01-15 00:00:00

  • Redox Aspects of Chaperones in Cardiac Function.

    abstract::Molecular chaperones are stress proteins that allow the correct folding or unfolding as well as the assembly or disassembly of macromolecular cellular components. Changes in expression and post-translational modifications of chaperones have been linked to a number of age- and stress-related diseases including cancer, ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2018.00216

    authors: Penna C,Sorge M,Femminò S,Pagliaro P,Brancaccio M

    更新日期:2018-03-16 00:00:00

  • Improvement of Muscle Quality of Grass Carp (Ctenopharyngodon idellus) With a Bio-Floating Bed in Culture Ponds.

    abstract::Muscle quality and the physiological condition of fish are affected greatly by the culture environment. In aquaculture ponds, a bio-floating bed planted with vegetation is often used to improve water quality. This study investigated the growth and muscle quality of grass carp (Ctenopharyngodon idellus) cultured in pon...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00683

    authors: Zhang X,Wang J,Tang R,He X,Li L,Takagi Y,Li D

    更新日期:2019-05-31 00:00:00

  • Nociceptors as chronic drivers of pain and hyperreflexia after spinal cord injury: an adaptive-maladaptive hyperfunctional state hypothesis.

    abstract::Spinal cord injury (SCI) causes chronic peripheral sensitization of nociceptors and persistent generation of spontaneous action potentials (SA) in peripheral branches and the somata of hyperexcitable nociceptors within dorsal root ganglia (DRG). Here it is proposed that SCI triggers in numerous nociceptors a persisten...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00309

    authors: Walters ET

    更新日期:2012-08-02 00:00:00

  • Functional Study of Cytochrome P450 Enzymes from the Brown Planthopper (Nilaparvata lugens Stål) to Analyze Its Adaptation to BPH-Resistant Rice.

    abstract::Plant-insect interactions constitute a complex of system, whereby plants synthesize toxic compounds as the main defense strategy to combat herbivore assault, and insects deploy detoxification systems to cope with toxic plant compounds. Cytochrom P450s are among the main detoxification enzymes employed by insects to co...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2017.00972

    authors: Peng L,Zhao Y,Wang H,Song C,Shangguan X,Ma Y,Zhu L,He G

    更新日期:2017-11-30 00:00:00

  • Application of Bioelectrical Impedance Analysis to Detect Broiler Breast Filets Affected With Woody Breast Myopathy.

    abstract::Woody breast (WB) myopathy in modern broilers is causing major meat quality issues and consumer complaints. The poultry industry is sorting out WB filets through the inconsistent manual hand-palpation method. Bioelectrical impedance analysis (BIA) method was evaluated as a rapid and objective WB detection method. Fres...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00808

    authors: Morey A,Smith AE,Garner LJ,Cox MK

    更新日期:2020-07-10 00:00:00

  • High-Intensity Interval Training: A Potential Exercise Countermeasure During Human Spaceflight.

    abstract::High-intensity interval training (HIT) is an effective approach for improving a range of physiological markers associated with physical fitness. A considerable body of work has demonstrated substantial improvements in cardiorespiratory fitness following short-term training programmes, while emerging evidence suggests ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2019.00581

    authors: Hurst C,Scott JPR,Weston KL,Weston M

    更新日期:2019-05-22 00:00:00

  • Non-invasive Assessment of Systolic and Diastolic Cardiac Function During Rest and Stress Conditions Using an Integrated Image-Modeling Approach.

    abstract::Background: The possibility of non-invasively assessing load-independent parameters characterizing cardiac function is of high clinical value. Typically, these parameters are assessed during resting conditions. However, for diagnostic purposes, the parameter behavior across a physiologically relevant range of heart ra...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01515

    authors: Casas B,Viola F,Cedersund G,Bolger AF,Karlsson M,Carlhäll CJ,Ebbers T

    更新日期:2018-10-30 00:00:00

  • Perindopril and a Galectin-3 Inhibitor Improve Ischemic Heart Failure in Rabbits by Reducing Gal-3 Expression and Myocardial Fibrosis.

    abstract::Objective: Ventricular remodeling is considered the basis of heart failure and is involved in myocardial fibrosis. This study aimed to assess perindopril and a galectin-3 inhibitor (modified citrus pectin, MCP) for their effects on ventricular remodeling and myocardial fibrosis in rabbits with ischemic heart failure. ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00267

    authors: Li S,Li S,Hao X,Zhang Y,Deng W

    更新日期:2019-03-22 00:00:00

  • Insects With Survival Kits for Desiccation Tolerance Under Extreme Water Deficits.

    abstract::The year 2002 marked the tercentenary of Antonie van Leeuwenhoek's discovery of desiccation tolerance in animals. This remarkable phenomenon to sustain 'life' in the absence of water can be revived upon return of hydrating conditions. Today, coping with climate change-related factors, especially temperature-humidity i...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2018.01843

    authors: Thorat L,Nath BB

    更新日期:2018-12-21 00:00:00

  • Association of Hyperuricemia With Immune Disorders and Intestinal Barrier Dysfunction.

    abstract:Background:More than 30-40% of uric acid is excreted via the intestine, and the dysfunction of intestinal epithelium disrupts uric acid excretion. The involvement of gut microbiota in hyperuricemia has been reported in previous studies, but the changes and mechanisms of intestinal immunity in hyperuricemia are still un...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.524236

    authors: Lv Q,Xu D,Zhang X,Yang X,Zhao P,Cui X,Liu X,Yang W,Yang G,Xing S

    更新日期:2020-11-27 00:00:00

  • Eicosapentaenoic acid attenuates cigarette smoke-induced lung inflammation by inhibiting ROS-sensitive inflammatory signaling.

    abstract::Cigarette smoking causes chronic lung inflammation that is mainly regulated by redox-sensitive pathways. Our previous studies have demonstrated that cigarette smoke (CS) activates reactive oxygen species (ROS)-sensitive mitogen-activated protein kinases (MAPKs)/nuclear factor-κB (NF-κB) signaling resulting in inductio...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2014.00440

    authors: Liu MH,Lin AH,Lu SH,Peng RY,Lee TS,Kou YR

    更新日期:2014-11-14 00:00:00

  • Limb-Specific Features and Asymmetry of Nerve Conduction Velocity and Nerve Trunk Size in Human.

    abstract::This study aimed to simultaneously examine the differences of human nerve conduction velocity (NCV) and nerve cross-sectional area (nCSA) between the upper and lower limbs and between different regions of the upper and lower limbs. Thirty healthy subjects volunteered for the study. NCV and nCSA of the ulnar and tibial...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.609006

    authors: Nobue A,Kunimasa Y,Tsuneishi H,Sano K,Oda H,Ishikawa M

    更新日期:2020-12-03 00:00:00

  • Modeling Pulmonary Gas Exchange and Single-Exhalation Profiles of Carbon Monoxide.

    abstract::Exhaled breath carbon monoxide (eCO) is a candidate biomarker for non-invasive assessment of oxidative stress and respiratory diseases. Standard end-tidal CO analysis, however, cannot distinguish, whether eCO reflects endogenous CO production, lung diffusion properties or exogenous sources, and is unable to resolve a ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00927

    authors: Ghorbani R,Blomberg A,Schmidt FM

    更新日期:2018-07-30 00:00:00

  • Salidroside Attenuates Denervation-Induced Skeletal Muscle Atrophy Through Negative Regulation of Pro-inflammatory Cytokine.

    abstract::Skeletal muscle atrophy is associated with pro-inflammatory cytokines. Salidroside is a biologically active ingredient of Rhodiola rosea, which exhibits anti-inflammatory property. However, there is little known about the effect of salidroside on denervation-induced muscle atrophy. Therefore, the present study aimed t...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00665

    authors: Wu C,Tang L,Ni X,Xu T,Fang Q,Xu L,Ma W,Yang X,Sun H

    更新日期:2019-06-25 00:00:00

  • Inhibition of the NFATc4/ERK/AKT Pathway and Improvement of Thiol-Specific Oxidative Stress by Dronedarone Possibly Secondary to the Reduction of Blood Pressure in an Animal Model of Ventricular Hypertrophy.

    abstract::Untreated chronic hypertension causes left ventricular hypertrophy, which is related to the occurrence of atrial fibrillation. Dronedarone is an antiarrhythmic agent recently approved for atrial fibrillation. Our group previously demonstrated that dronedarone produced an early regression of left ventricular hypertroph...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00967

    authors: Pazó-Sayós L,González MC,Quintana-Villamandos B

    更新日期:2020-08-26 00:00:00