Abstract:
BACKGROUND:Kawasaki disease (KD) is a prevalent pediatric disease worldwide and can cause coronary artery aneurysm as a severe complication. Typically, DNA methylation is thought to repress the expression of nearby genes. However, the cases in which DNA methylation promotes gene expression have been reported. In addition, globally, to what extent DNA methylation affects gene expression and how it contributes to the pathogenesis of KD are not yet well understood. METHODS:To address these important biological questions, we enrolled subjects, collected DNA and RNA samples from the subjects' total white blood cells, and performed DNA methylation (M450K) and gene expression (HTA 2.0) microarray assays. RESULTS:By analyzing the variation ratios of CpG beta values (methylation percentage) and gene expression intensities, we first concluded that the CpG markers close (- 1500 bp to + 500 bp) to the transcription start sites had higher variation ratios, reflecting significant regulation capacities. Next, we observed that, globally speaking, gene expression was modestly negatively correlated (correlation rho ≈ - 0.2) with the DNA methylation status of both upstream and downstream CpG markers in the promoter region. Third, we found that specific CpG markers were hypo-methylated in disease samples compared with healthy samples and hyper-methylated in convalescent samples compared with disease samples, promoting and repressing S100A genes' expressions, respectively. Finally, using an in vitro cell model, we demonstrated that S100A family proteins enhanced leukocyte transendothelial migration in KD. CONCLUSIONS:This is the first study to integrate genome-wide DNA methylation with gene expression assays in KD and showed that the S100A family plays important roles in the pathogenesis of KD.
journal_name
Clin Epigeneticsjournal_title
Clinical epigeneticsauthors
Huang LH,Kuo HC,Pan CT,Lin YS,Huang YH,Li SCdoi
10.1186/s13148-018-0557-1subject
Has Abstractpub_date
2018-11-01 00:00:00pages
135issue
1eissn
1868-7075issn
1868-7083pii
10.1186/s13148-018-0557-1journal_volume
10pub_type
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journal_title:Clinical epigenetics
pub_type: 杂志文章,评审
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更新日期:2015-04-10 00:00:00
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pub_type: 杂志文章
doi:10.1186/s13148-019-0694-1
更新日期:2019-06-28 00:00:00
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pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2016-03-15 00:00:00
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pub_type: 杂志文章
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journal_title:Clinical epigenetics
pub_type: 已发布勘误
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更新日期:2019-10-21 00:00:00
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journal_title:Clinical epigenetics
pub_type: 临床试验,杂志文章
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2017-05-30 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-014-0032-6
更新日期:2014-12-13 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0368-9
更新日期:2017-07-14 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/1868-7083-5-18
更新日期:2013-10-03 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00933-2
更新日期:2020-09-11 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-015-0109-x
更新日期:2015-07-23 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-018-0501-4
更新日期:2018-05-23 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1007/s13148-011-0029-3
更新日期:2011-08-01 00:00:00