Early detection of colorectal cancer based on presence of methylated syndecan-2 (SDC2) in stool DNA.


BACKGROUND:Colorectal cancer (CRC) screening can effectively reduce disease-related mortality by detecting CRC at earlier stages. We have previously demonstrated that the presence of SDC2 methylation in stool DNA is significantly associated with the occurrence of CRC regardless of clinical stage. The aim of this study was to evaluate the clinical performance of stool DNA-based SDC2 methylation test for CRC. METHODS:Aberrant SDC2 methylation in stool-derived DNA was measured by linear target enrichment (LTE)-quantitative methylation-specific real-time PCR (qMSP). Duplicate reactions of meSDC2 LTE-qMSP test were performed for stool samples obtained from CRC patients representing all stages (0-IV) and asymptomatic individuals who were subsequently underwent colonoscopy examination. To determine the diagnostic value of test in CRC and control groups, sensitivity and specificity were evaluated by receiver operating characteristic curve analysis. RESULTS:Of 585 subjects who could be evaluated, 245 had CRC, 44 had various sizes of adenomatous polyps, and 245 had negative colonoscopy results. Stool DNA-based meSDC2 LTE-qMSP showed an overall sensitivity of 90.2% with AUC of 0.902 in detecting CRC (0-IV) not associated with tumor stage, location, sex, or age (P > 0.05), with a specificity of 90.2%. Sensitivity for detecting early stages (0-II) was 89.1% (114/128). This test also detected 66.7% (2/3) and 24.4% (10/41) of advanced and non-advanced adenomas, respectively. CONCLUSIONS:Results of this study validated the capability of stool DNA based-SDC2 methylation test by LTE-qMSP for early detection of CRC patient with high specificity. TRIAL REGISTRATION:ClinicalTrials.gov, NCT03146520 , Registered 10 May 2017, Retrospectively registered; however, control arm was prospectively registered.


Clin Epigenetics


Clinical epigenetics


Han YD,Oh TJ,Chung TH,Jang HW,Kim YN,An S,Kim NK




Has Abstract


2019-03-15 00:00:00














  • RFX1 downregulation contributes to TLR4 overexpression in CD14+ monocytes via epigenetic mechanisms in coronary artery disease.

    abstract:BACKGROUND:Toll-like receptor 4 (TLR4) expression is increased in activated monocytes, which play a critical role in the pathogenesis of coronary artery disease (CAD). However, the mechanism remains unclear. Regulatory factor X1 (RFX1) is a critical transcription factor regulating epigenetic modifications. In this stud...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Du P,Gao K,Cao Y,Yang S,Wang Y,Guo R,Zhao M,Jia S

    更新日期:2019-03-11 00:00:00

  • Correction to: DNA methylation signature is prognostic of choroid plexus tumor aggressiveness.

    abstract::After publication of the original article [1], authors have requested to add a 'J' as middle name for Richard Gilbertson. Hence, full name should be Richard J Gilbertson. ...

    journal_title:Clinical epigenetics

    pub_type: 已发布勘误


    authors: Pienkowska M,Choufani S,Turinsky AL,Guha T,Merino DM,Novokmet A,Brudno M,Weksberg R,Shlien A,Hawkins C,Bouffet E,Tabori U,Gilbertson RJ,Finlay JL,Jabado N,Thomas C,Sill M,Capper D,Hasselblatt M,Malkin D

    更新日期:2019-10-21 00:00:00

  • Epigenomic profiling of prostate cancer identifies differentially methylated genes in TMPRSS2:ERG fusion-positive versus fusion-negative tumors.

    abstract:BACKGROUND:About half of all prostate cancers harbor the TMPRSS2:ERG (T2E) gene fusion. While T2E-positive and T2E-negative tumors represent specific molecular subtypes of prostate cancer (PCa), previous studies have not yet comprehensively investigated how these tumor subtypes differ at the epigenetic level. We theref...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Geybels MS,Alumkal JJ,Luedeke M,Rinckleb A,Zhao S,Shui IM,Bibikova M,Klotzle B,van den Brandt PA,Ostrander EA,Fan JB,Feng Z,Maier C,Stanford JL

    更新日期:2015-12-12 00:00:00

  • Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure.

    abstract:BACKGROUND:Alcohol consumption during pregnancy is a significant public health problem and can result in a continuum of adverse outcomes to the fetus known as fetal alcohol spectrum disorders (FASD). Subjects with FASD show significant neurological deficits, ranging from microencephaly, neurobehavioral, and mental heal...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Rachdaoui N,Li L,Willard B,Kasumov T,Previs S,Sarkar D

    更新日期:2017-10-23 00:00:00

  • Longitudinal epigenome-wide association studies of three male military cohorts reveal multiple CpG sites associated with post-traumatic stress disorder.

    abstract:BACKGROUND:Epigenetic mechanisms have been suggested to play a role in the development of post-traumatic stress disorder (PTSD). Here, blood-derived DNA methylation data (HumanMethylation450 BeadChip) collected prior to and following combat exposure in three cohorts of male military members were analyzed to assess whet...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Snijders C,Maihofer AX,Ratanatharathorn A,Baker DG,Boks MP,Geuze E,Jain S,Kessler RC,Pishva E,Risbrough VB,Stein MB,Ursano RJ,Vermetten E,Vinkers CH,PGC PTSD EWAS Consortium.,Smith AK,Uddin M,Rutten BPF,Nievergelt CM

    更新日期:2020-01-13 00:00:00

  • Aberrant DNA methylation and expression of SPDEF and FOXA2 in airway epithelium of patients with COPD.

    abstract:BACKGROUND:Goblet cell metaplasia, a common feature of chronic obstructive pulmonary disease (COPD), is associated with mucus hypersecretion which contributes to the morbidity and mortality among patients. Transcription factors SAM-pointed domain-containing Ets-like factor (SPDEF) and forkhead box protein A2 (FOXA2) re...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Song J,Heijink IH,Kistemaker LEM,Reinders-Luinge M,Kooistra W,Noordhoek JA,Gosens R,Brandsma CA,Timens W,Hiemstra PS,Rots MG,Hylkema MN

    更新日期:2017-04-24 00:00:00

  • DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer.

    abstract:BACKGROUND:Colorectal cancer (CRC) is the third most commonly diagnosed human malignancy worldwide. Upregulation of inhibitory immune checkpoints by tumor-infiltrating immune cells (TIICs) or their ligands by tumor cells leads to tumor evasion from host immunosurveillance. Changes in DNA methylation pattern and enrichm...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Sasidharan Nair V,Toor SM,Taha RZ,Shaath H,Elkord E

    更新日期:2018-08-06 00:00:00

  • Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots.

    abstract::Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RR...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Ingerslev LR,Donkin I,Fabre O,Versteyhe S,Mechta M,Pattamaprapanont P,Mortensen B,Krarup NT,Barrès R

    更新日期:2018-01-25 00:00:00

  • H3K4 tri-methylation breadth at transcription start sites impacts the transcriptome of systemic lupus erythematosus.

    abstract:BACKGROUND:The autoimmune disease systemic lupus erythematosus (SLE) has a modified epigenome with modified tri-methylation of histone H3 lysine 4 (H3K4me3) at specific loci across the genome. H3K4me3 is a canonical chromatin mark of active transcription. Recent studies have suggested that H3K4me3 breadth has an import...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Zhang Z,Shi L,Dawany N,Kelsen J,Petri MA,Sullivan KE

    更新日期:2016-02-02 00:00:00

  • Genetic, epigenetic, and environmental factors controlling oxytocin receptor gene expression.

    abstract:BACKGROUND:The neuropeptide oxytocin regulates mammalian social behavior. Disruptions in oxytocin signaling are a feature of many psychopathologies. One commonly studied biomarker for oxytocin involvement in psychiatric diseases is DNA methylation at the oxytocin receptor gene (OXTR). Such studies focus on DNA methylat...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Danoff JS,Wroblewski KL,Graves AJ,Quinn GC,Perkeybile AM,Kenkel WM,Lillard TS,Parikh HI,Golino HF,Gregory SG,Carter CS,Bales KL,Connelly JJ

    更新日期:2021-01-30 00:00:00

  • Dynamic interplay between locus-specific DNA methylation and hydroxymethylation regulates distinct biological pathways in prostate carcinogenesis.

    abstract:BACKGROUND:Despite the significant global loss of DNA hydroxymethylation marks in prostate cancer tissues, the locus-specific role of hydroxymethylation in prostate tumorigenesis is unknown. We characterized hydroxymethylation and methylation marks by performing whole-genome next-generation sequencing in representative...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Kamdar SN,Ho LT,Kron KJ,Isserlin R,van der Kwast T,Zlotta AR,Fleshner NE,Bader G,Bapat B

    更新日期:2016-03-15 00:00:00

  • TWIST1 DNA methylation is a cell marker of airway and parenchymal lung fibroblasts that are differentially methylated in asthma.

    abstract:BACKGROUND:Mesenchymal fibroblasts are ubiquitous cells that maintain the extracellular matrix of organs. Within the lung, airway and parenchymal fibroblasts are crucial for lung development and are altered with disease, but it has been difficult to understand their roles due to the lack of distinct molecular markers. ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Clifford RL,Yang CX,Fishbane N,Patel J,MacIsaac JL,McEwen LM,May ST,Castellanos-Uribe M,Nair P,Obeidat M,Kobor MS,Knox AJ,Hackett TL

    更新日期:2020-10-02 00:00:00

  • Individual epigenetic status of the pathogenic D4Z4 macrosatellite correlates with disease in facioscapulohumeral muscular dystrophy.

    abstract:BACKGROUND:Both forms of facioscapulohumeral muscular dystrophy (FSHD) are associated with aberrant epigenetic regulation of the chromosome 4q35 D4Z4 macrosatellite. Chromatin changes due to large deletions of heterochromatin (FSHD1) or mutations in chromatin regulatory proteins (FSHD2) lead to relaxation of epigenetic...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Jones TI,King OD,Himeda CL,Homma S,Chen JC,Beermann ML,Yan C,Emerson CP Jr,Miller JB,Wagner KR,Jones PL

    更新日期:2015-03-29 00:00:00

  • DNA methylation signature of interleukin 1 receptor type II in asthma.

    abstract::Interleukin 1 and its receptors are associated with allergic diseases such as asthma. In the present study, we measured DNA methylation at the IL1R1 and IL1R2 gene loci and assessed for associations with asthma-related phenotypes and gene expressions. We found that asthmatic and atopic individuals have higher IL1R2 pr...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Gagné-Ouellet V,Guay SP,Boucher-Lafleur AM,Bouchard L,Laprise C

    更新日期:2015-08-05 00:00:00

  • Genome-wide DNA methylation profiles of low- and high-grade adenoma reveals potential biomarkers for early detection of colorectal carcinoma.

    abstract:BACKGROUND:Abnormal DNA methylation is a hallmark of human cancers and may be a promising biomarker for early diagnosis of human cancers. However, the majority of DNA methylation biomarkers that have been identified are based on the hypothesis that early differential methylation regions (DMRs) are maintained throughout...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Fan J,Li J,Guo S,Tao C,Zhang H,Wang W,Zhang Y,Zhang D,Ding S,Zeng C

    更新日期:2020-04-21 00:00:00

  • Integrative DNA methylome analysis of pan-cancer biomarkers in cancer discordant monozygotic twin-pairs.

    abstract:BACKGROUND:A key focus in cancer research is the discovery of biomarkers that accurately diagnose early lesions in non-invasive tissues. Several studies have identified malignancy-associated DNA methylation changes in blood, yet no general cancer biomarker has been identified to date. Here, we explore the potential of ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Roos L,van Dongen J,Bell CG,Burri A,Deloukas P,Boomsma DI,Spector TD,Bell JT

    更新日期:2016-01-20 00:00:00

  • Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis.

    abstract:Background:The dynamic methylation of human papillomavirus (HPV) 16 DNA is thought to be associated with the progression of cervical lesions. Previous studies that did not consider the physical status of HPV 16 may have incorrectly mapped HPV 16 methylomes. In order to identify reliable biomarkers for squamous cervical...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Liu L,Ying C,Zhao Z,Sui L,Zhang X,Qian C,Wang Q,Chen L,Guo Q,Wu J

    更新日期:2018-01-23 00:00:00

  • Gene domain-specific DNA methylation episignatures highlight distinct molecular entities of ADNP syndrome.

    abstract:BACKGROUND:ADNP syndrome is a rare Mendelian disorder characterized by global developmental delay, intellectual disability, and autism. It is caused by truncating mutations in ADNP, which is involved in chromatin regulation. We hypothesized that the disruption of chromatin regulation might result in specific DNA methyl...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Bend EG,Aref-Eshghi E,Everman DB,Rogers RC,Cathey SS,Prijoles EJ,Lyons MJ,Davis H,Clarkson K,Gripp KW,Li D,Bhoj E,Zackai E,Mark P,Hakonarson H,Demmer LA,Levy MA,Kerkhof J,Stuart A,Rodenhiser D,Friez MJ,Stevenson

    更新日期:2019-04-27 00:00:00

  • Epigenetic management of major psychosis.

    abstract::Epigenetic mechanisms are thought to play a major role in the pathogenesis of the major psychoses (schizophrenia and bipolar disorder), and they may be the link between the environment and the genome in the pathogenesis of these disorders. This paper discusses the role of epigenetics in the management of major psychos...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Peedicayil J

    更新日期:2011-08-01 00:00:00

  • SLCO4C1 promoter methylation is a potential biomarker for prognosis associated with biochemical recurrence-free survival after radical prostatectomy.

    abstract:BACKGROUND:Prostate cancer (PC) is a commonly diagnosed malignancy in males, especially in the western hemisphere. The extensive use of multiple biomarkers plays an important role in the diagnosis and prognosis of PC. However, the accuracy of biomarkers for PC prognosis needs to be urgently improved. This study aimed t...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Li X,Zhang W,Song J,Zhang X,Ran L,He Y

    更新日期:2019-07-09 00:00:00

  • Epigenetic stratification of head and neck cancer survivors reveals differences in lycopene levels, alcohol consumption, and methylation of immune regulatory genes.

    abstract:BACKGROUND:Inflammation has been associated with higher rates of recurrence and mortality in head and neck cancer (HNC). While the biological mechanisms predisposing patients to heightened inflammatory states remain largely unknown, DNA methylation has been proposed to reflect systemic inflammation. In this analysis, w...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Moody L,Crowder SL,Fruge AD,Locher JL,Demark-Wahnefried W,Rogers LQ,Delk-Licata A,Carroll WR,Spencer SA,Black M,Erdman JW Jr,Chen H,Pan YX,Arthur AE

    更新日期:2020-09-11 00:00:00

  • MUC1 is associated with TFF2 methylation in gastric cancer.

    abstract:BACKGROUND:Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1-/- mice compared with controls. However, little is known of the molecul...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Ge Y,Ma G,Liu H,Lin Y,Zhang G,Du M,Wang M,Chu H,Zhang H,Zhang Z

    更新日期:2020-03-02 00:00:00

  • Epigenetic suppression of E-cadherin expression by Snail2 during the metastasis of colorectal cancer.

    abstract:BACKGROUND:The transcription factor Snail2 is a repressor of E-cadherin expression during carcinogenesis; however, the specific mechanisms involved in this process in human colorectal cancer (CRC) remain largely unknown. METHOD:We checked the expression of Snail2 in several clinical CRC specimens. Then, we established...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Hu Y,Dai M,Zheng Y,Wu J,Yu B,Zhang H,Kong W,Wu H,Yu X

    更新日期:2018-12-12 00:00:00

  • Overall and sex-specific associations between methylation of the ABCG1 and APOE genes and ischemic stroke or other atherosclerosis-related traits in a sibling study of Chinese population.

    abstract:BACKGROUND:Identifying subjects with a high risk of ischemic stroke is fundamental for prevention of the disease. Both genetic and environmental risk factors contribute to ischemic stroke, but the underlying epigenetic mechanisms which mediate genetic and environmental risk effects are not fully understood. The aim of ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Qin X,Li J,Wu T,Wu Y,Tang X,Gao P,Li L,Wang M,Wu Y,Wang X,Chen D,Hu Y

    更新日期:2019-12-10 00:00:00

  • Sperm imprinting integrity in seminoma patients?

    abstract:BACKGROUND:Testicular germ cell tumor such as seminoma is strongly associated with male reproductive problems commonly associated with the alteration of sperm parameters as described in testicular dysgenesis syndrome. Interestingly, numerous studies have reported that the precursor of germ cell cancer, germ cell neopla...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Bruno C,Blagoskonov O,Barberet J,Guilleman M,Daniel S,Tournier B,Binquet C,Choux C,Fauque P

    更新日期:2018-10-19 00:00:00

  • Maternal diabetes and obesity influence the fetal epigenome in a largely Hispanic population.

    abstract:BACKGROUND:Obesity and diabetes mellitus are directly implicated in many adverse health consequences in adults as well as in the offspring of obese and diabetic mothers. Hispanic Americans are particularly at risk for obesity, diabetes, and end-stage renal disease. Maternal obesity and/or diabetes through prenatal prog...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Rizzo HE,Escaname EN,Alana NB,Lavender E,Gelfond J,Fernandez R,Hibbs MA,King JM,Carr NR,Blanco CL

    更新日期:2020-02-19 00:00:00

  • Cooperative effect of chidamide and chemotherapeutic drugs induce apoptosis by DNA damage accumulation and repair defects in acute myeloid leukemia stem and progenitor cells.

    abstract:BACKGROUND:Many conventional chemotherapeutic drugs are known to be involved in DNA damage, thus ultimately leading to apoptosis of leukemic cells. However, they fail to completely eliminate leukemia stem cells (LSCs) due to their higher DNA repair capacity of cancer stem cells than that of bulk cancer cells, which bec...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Li Y,Wang Y,Zhou Y,Li J,Chen K,Zhang L,Deng M,Deng S,Li P,Xu B

    更新日期:2017-08-14 00:00:00

  • Differential placental methylation and expression of VEGF, FLT-1 and KDR genes in human term and preterm preeclampsia.

    abstract:BACKGROUND:Preeclampsia, a pregnancy complication of placental origin is associated with altered expression of angiogenic factors and their receptors. Recently, there is considerable interest in understanding the role of adverse intrauterine conditions in placental dysfunction and adverse pregnancy outcomes. Since we h...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Sundrani DP,Reddy US,Joshi AA,Mehendale SS,Chavan-Gautam PM,Hardikar AA,Chandak GR,Joshi SR

    更新日期:2013-04-26 00:00:00

  • Genome-wide assessment of DNA methylation in mouse oocytes reveals effects associated with in vitro growth, superovulation, and sexual maturity.

    abstract:BACKGROUND:In vitro follicle culture (IFC), as applied in the mouse system, allows the growth and maturation of a large number of immature preantral follicles to become mature and competent oocytes. In the human oncofertility clinic, there is increasing interest in developing this technique as an alternative to ovarian...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Saenz-de-Juano MD,Ivanova E,Billooye K,Herta AC,Smitz J,Kelsey G,Anckaert E

    更新日期:2019-12-19 00:00:00

  • Alternative methylation of intron motifs is associated with cancer-related gene expression in both canine mammary tumor and human breast cancer.

    abstract:BACKGROUND:Canine mammary tumor (CMT) has long been considered as a good animal model for human breast cancer (HBC) due to their pathological and biological similarities. However, only a few aspects of the epigenome have been explored in both HBC and CMT. Moreover, DNA methylation studies have mainly been limited to th...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Nam AR,Lee KH,Hwang HJ,Schabort JJ,An JH,Won SH,Cho JY

    更新日期:2020-07-21 00:00:00