Epigenetic suppression of E-cadherin expression by Snail2 during the metastasis of colorectal cancer.


BACKGROUND:The transcription factor Snail2 is a repressor of E-cadherin expression during carcinogenesis; however, the specific mechanisms involved in this process in human colorectal cancer (CRC) remain largely unknown. METHOD:We checked the expression of Snail2 in several clinical CRC specimens. Then, we established Snail2-overexpressing and knockdown cell lines to determine the function of Snail2 during EMT and metastasis processes in CRC. In addition, we used luciferase reporter assay to explore how Snail2 inhibits the expression of E-cadherin and induces EMT. RESULTS:We found that the expression of Snail2 was higher in clinical specimens of colorectal cancer (CRC) compared to non-cancerous tissues. Overexpression of Snail2 induced migration and metastatic properties in CRC cells in vitro and in vivo. Furthermore, overexpression of Snail2 promoted the occurrence of the epithelial-mesenchymal transition (EMT), downregulating the expression of E-cadherin and upregulating that of vimentin. Specifically, Snail2 could interact with HDAC6 and then recruited HDAC6 and PRC2 to the promoter of E-cadherin and thus inhibited the expression of E-cadherin, promoting EMT and inducing invasion and metastasis of CRC. CONCLUSION:Our study reveals that Snail2 might epigenetically suppress the expression of E-cadherin during CRC metastasis.


Clin Epigenetics


Clinical epigenetics


Hu Y,Dai M,Zheng Y,Wu J,Yu B,Zhang H,Kong W,Wu H,Yu X




Has Abstract


2018-12-12 00:00:00














  • Altered DNA methylation is associated with aberrant gene expression in parenchymal but not airway fibroblasts isolated from individuals with COPD.

    abstract:Background:Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease of the lungs that is currently the fourth leading cause of death worldwide. Genetic factors account for only a small amount of COPD risk, but epigenetic mechanisms, including DNA methylation, have the potential to mediate the interaction...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Clifford RL,Fishbane N,Patel J,MacIsaac JL,McEwen LM,Fisher AJ,Brandsma CA,Nair P,Kobor MS,Hackett TL,Knox AJ

    更新日期:2018-03-05 00:00:00

  • Cooperative effect of chidamide and chemotherapeutic drugs induce apoptosis by DNA damage accumulation and repair defects in acute myeloid leukemia stem and progenitor cells.

    abstract:BACKGROUND:Many conventional chemotherapeutic drugs are known to be involved in DNA damage, thus ultimately leading to apoptosis of leukemic cells. However, they fail to completely eliminate leukemia stem cells (LSCs) due to their higher DNA repair capacity of cancer stem cells than that of bulk cancer cells, which bec...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Li Y,Wang Y,Zhou Y,Li J,Chen K,Zhang L,Deng M,Deng S,Li P,Xu B

    更新日期:2017-08-14 00:00:00

  • The effects of olanzapine on genome-wide DNA methylation in the hippocampus and cerebellum.

    abstract:BACKGROUND:The mechanism of action of olanzapine in treating schizophrenia is not clear. This research reports the effects of a therapeutic equivalent treatment of olanzapine on DNA methylation in a rat model in vivo.Genome-wide DNA methylation was assessed using a MeDIP-chip analysis. All methylated DNA immunoprecipit...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Melka MG,Laufer BI,McDonald P,Castellani CA,Rajakumar N,O'Reilly R,Singh SM

    更新日期:2014-01-02 00:00:00

  • High-resolution analysis of germ cells from men with sex chromosomal aneuploidies reveals normal transcriptome but impaired imprinting.

    abstract:BACKGROUND:The most common sex chromosomal aneuploidy in males is Klinefelter syndrome, which is characterized by at least one supernumerary X chromosome. While these men have long been considered infertile, focal spermatogenesis can be observed in some patients, and sperm can be surgically retrieved and used for artif...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Laurentino S,Heckmann L,Di Persio S,Li X,Meyer Zu Hörste G,Wistuba J,Cremers JF,Gromoll J,Kliesch S,Schlatt S,Neuhaus N

    更新日期:2019-08-28 00:00:00

  • Characterisation of an inflammation-related epigenetic score and its association with cognitive ability.

    abstract:BACKGROUND:Chronic systemic inflammation has been associated with incident dementia, but its association with age-related cognitive decline is less clear. The acute responses of many inflammatory biomarkers mean they may provide an unreliable picture of the chronicity of inflammation. Recently, a large-scale epigenome-...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Stevenson AJ,McCartney DL,Hillary RF,Campbell A,Morris SW,Bermingham ML,Walker RM,Evans KL,Boutin TS,Hayward C,McRae AF,McColl BW,Spires-Jones TL,McIntosh AM,Deary IJ,Marioni RE

    更新日期:2020-07-27 00:00:00

  • Nucleosome positioning is unaltered at MLH1 splice site mutations in cells derived from Lynch syndrome patients.

    abstract:BACKGROUND:Splicing is more efficient when coupled with transcription and it has been proposed that nucleosomes enriched in exons are important for splice site recognition. Lynch syndrome is a familial cancer syndrome that can be caused by the autosomal dominant inheritance of splice site mutations in the MutL homolog ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Sloane MA,Hesson LB,Nunez AC,Thompson BA,Ward RL

    更新日期:2014-12-13 00:00:00

  • Reduced mitochondrial D-loop methylation levels in sporadic amyotrophic lateral sclerosis.

    abstract:BACKGROUND:Mitochondrial dysregulation and aberrant epigenetic mechanisms have been frequently reported in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), and several researchers suggested that epigenetic dysregulation in mitochondrial DNA (mtDNA) could contribute to the neurodegenerative pro...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Stoccoro A,Smith AR,Mosca L,Marocchi A,Gerardi F,Lunetta C,Cereda C,Gagliardi S,Lunnon K,Migliore L,Coppedè F

    更新日期:2020-09-11 00:00:00

  • Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers.

    abstract:BACKGROUND:The DNA modification 5-hydroxymethylcytosine (5hmC) is now referred to as the sixth base of DNA with evidence of tissue-specific patterns and correlation with gene regulation and expression. This epigenetic mark was recently reported as a potential biomarker for multiple types of cancer, but its application ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Gilat N,Tabachnik T,Shwartz A,Shahal T,Torchinsky D,Michaeli Y,Nifker G,Zirkin S,Ebenstein Y

    更新日期:2017-07-14 00:00:00

  • Increased epigenetic age in normal breast tissue from luminal breast cancer patients.

    abstract:BACKGROUND:Age is one of the most important risk factors for developing breast cancer. However, age-related changes in normal breast tissue that potentially lead to breast cancer are incompletely understood. Quantifying tissue-level DNA methylation can contribute to understanding these processes. We hypothesized that o...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章,多中心研究


    authors: Hofstatter EW,Horvath S,Dalela D,Gupta P,Chagpar AB,Wali VB,Bossuyt V,Storniolo AM,Hatzis C,Patwardhan G,Von Wahlde MK,Butler M,Epstein L,Stavris K,Sturrock T,Au A,Kwei S,Pusztai L

    更新日期:2018-08-29 00:00:00

  • DNA hydroxymethylation age of human blood determined by capillary hydrophilic-interaction liquid chromatography/mass spectrometry.

    abstract:BACKGROUND:Aging is a complex phenomenon and characterized by a progressive decline in physiology and function of adult tissues. However, it hasn't been well established of the correlation between aging and global DNA methylation and hydroxymethylation that regulate the growth and development of higher organisms. RESU...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Xiong J,Jiang HP,Peng CY,Deng QY,Lan MD,Zeng H,Zheng F,Feng YQ,Yuan BF

    更新日期:2015-07-23 00:00:00

  • Epigenetic stratification of head and neck cancer survivors reveals differences in lycopene levels, alcohol consumption, and methylation of immune regulatory genes.

    abstract:BACKGROUND:Inflammation has been associated with higher rates of recurrence and mortality in head and neck cancer (HNC). While the biological mechanisms predisposing patients to heightened inflammatory states remain largely unknown, DNA methylation has been proposed to reflect systemic inflammation. In this analysis, w...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Moody L,Crowder SL,Fruge AD,Locher JL,Demark-Wahnefried W,Rogers LQ,Delk-Licata A,Carroll WR,Spencer SA,Black M,Erdman JW Jr,Chen H,Pan YX,Arthur AE

    更新日期:2020-09-11 00:00:00

  • The elements of human cyclin D1 promoter and regulation involved.

    abstract::Cyclin D1 is a cell cycle machine, a sensor of extracellular signals and plays an important role in G1-S phase progression. The human cyclin D1 promoter contains multiple transcription factor binding sites such as AP-1, NF-қB, E2F, Oct-1, and so on. The extracellular signals functions through the signal transduction p...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Guo ZY,Hao XH,Tan FF,Pei X,Shang LM,Jiang XL,Yang F

    更新日期:2011-08-01 00:00:00

  • DNA methylation profiling in peripheral lung tissues of smokers and patients with COPD.

    abstract:BACKGROUND:Epigenetics changes have been shown to be affected by cigarette smoking. Cigarette smoke (CS)-mediated DNA methylation can potentially affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in the lungs of patients with chronic obstructive pulmonary disease (COPD). We ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Sundar IK,Yin Q,Baier BS,Yan L,Mazur W,Li D,Susiarjo M,Rahman I

    更新日期:2017-04-14 00:00:00

  • Epigenetic clocks for gestational age: statistical and study design considerations.

    abstract::In this letter to the editor, we highlight some concerns with a recently published method to estimate gestational age at delivery from DNA methylation data. We conduct novel analyses to highlight the implications of different choices in study design and statistical methods for the prediction of phenotypes from methyla...

    journal_title:Clinical epigenetics

    pub_type: 信件


    authors: Simpkin AJ,Suderman M,Howe LD

    更新日期:2017-09-15 00:00:00

  • Targeted next-generation sequencing reveals high frequency of mutations in epigenetic regulators across treatment-naïve patient melanomas.

    abstract:BACKGROUND:Recent developments in genomic sequencing have advanced our understanding of the mutations underlying human malignancy. Melanoma is a prototype of an aggressive, genetically heterogeneous cancer notorious for its biologic plasticity and predilection towards developing resistance to targeted therapies. Eviden...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Lee JJ,Sholl LM,Lindeman NI,Granter SR,Laga AC,Shivdasani P,Chin G,Luke JJ,Ott PA,Hodi FS,Mihm MC Jr,Lin JY,Werchniak AE,Haynes HA,Bailey N,Liu R,Murphy GF,Lian CG

    更新日期:2015-06-09 00:00:00

  • Association of DNA methylation with age, gender, and smoking in an Arab population.

    abstract:BACKGROUND:Modification of DNA by methylation of cytosines at CpG dinucleotides is a widespread phenomenon that leads to changes in gene expression, thereby influencing and regulating many biological processes. Recent technical advances in the genome-wide determination of single-base DNA-methylation enabled epigenome-w...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Zaghlool SB,Al-Shafai M,Al Muftah WA,Kumar P,Falchi M,Suhre K

    更新日期:2015-01-22 00:00:00

  • Integrative DNA methylome analysis of pan-cancer biomarkers in cancer discordant monozygotic twin-pairs.

    abstract:BACKGROUND:A key focus in cancer research is the discovery of biomarkers that accurately diagnose early lesions in non-invasive tissues. Several studies have identified malignancy-associated DNA methylation changes in blood, yet no general cancer biomarker has been identified to date. Here, we explore the potential of ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Roos L,van Dongen J,Bell CG,Burri A,Deloukas P,Boomsma DI,Spector TD,Bell JT

    更新日期:2016-01-20 00:00:00

  • Comprehensive evaluation of targeted multiplex bisulphite PCR sequencing for validation of DNA methylation biomarker panels.

    abstract:BACKGROUND:DNA methylation is a well-studied epigenetic mark that is frequently altered in diseases such as cancer, where specific changes are known to reflect the type and severity of the disease. Therefore, there is a growing interest in assessing the clinical utility of DNA methylation as a biomarker for diagnosing ...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Lam D,Luu PL,Song JZ,Qu W,Risbridger GP,Lawrence MG,Lu J,Trau M,Korbie D,Clark SJ,Pidsley R,Stirzaker C

    更新日期:2020-06-22 00:00:00

  • T cell epigenetic remodeling and accelerated epigenetic aging are linked to long-term immune alterations in childhood cancer survivors.

    abstract:BACKGROUND:Cancer treatments have substantially improved childhood cancer survival but are accompanied by long-term complications, notably chronic inflammatory diseases. We hypothesize that cancer treatments could lead to long-term epigenetic changes in immune cells, resulting in increased prevalence of inflammatory di...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Daniel S,Nylander V,Ingerslev LR,Zhong L,Fabre O,Clifford B,Johnston K,Cohn RJ,Barres R,Simar D

    更新日期:2018-11-06 00:00:00

  • Genome-wide DNA methylation profiles of low- and high-grade adenoma reveals potential biomarkers for early detection of colorectal carcinoma.

    abstract:BACKGROUND:Abnormal DNA methylation is a hallmark of human cancers and may be a promising biomarker for early diagnosis of human cancers. However, the majority of DNA methylation biomarkers that have been identified are based on the hypothesis that early differential methylation regions (DMRs) are maintained throughout...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Fan J,Li J,Guo S,Tao C,Zhang H,Wang W,Zhang Y,Zhang D,Ding S,Zeng C

    更新日期:2020-04-21 00:00:00

  • Genome-wide assessment of DNA methylation in mouse oocytes reveals effects associated with in vitro growth, superovulation, and sexual maturity.

    abstract:BACKGROUND:In vitro follicle culture (IFC), as applied in the mouse system, allows the growth and maturation of a large number of immature preantral follicles to become mature and competent oocytes. In the human oncofertility clinic, there is increasing interest in developing this technique as an alternative to ovarian...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Saenz-de-Juano MD,Ivanova E,Billooye K,Herta AC,Smitz J,Kelsey G,Anckaert E

    更新日期:2019-12-19 00:00:00

  • Epigenetic modifications in muscle regeneration and progression of Duchenne muscular dystrophy.

    abstract::Duchenne muscular dystrophy (DMD) is a multisystemic disorder that affects 1:5000 boys. The severity of the phenotype varies dependent on the mutation site in the DMD gene and the resultant dystrophin expression profile. In skeletal muscle, dystrophin loss is associated with the disintegration of myofibers and their i...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章,评审


    authors: Rugowska A,Starosta A,Konieczny P

    更新日期:2021-01-19 00:00:00

  • Genetic, epigenetic, and environmental factors controlling oxytocin receptor gene expression.

    abstract:BACKGROUND:The neuropeptide oxytocin regulates mammalian social behavior. Disruptions in oxytocin signaling are a feature of many psychopathologies. One commonly studied biomarker for oxytocin involvement in psychiatric diseases is DNA methylation at the oxytocin receptor gene (OXTR). Such studies focus on DNA methylat...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Danoff JS,Wroblewski KL,Graves AJ,Quinn GC,Perkeybile AM,Kenkel WM,Lillard TS,Parikh HI,Golino HF,Gregory SG,Carter CS,Bales KL,Connelly JJ

    更新日期:2021-01-30 00:00:00

  • Characterisation of DNA methylation changes in EBF3 and TBC1D16 associated with tumour progression and metastasis in multiple cancer types.

    abstract:BACKGROUND:Characteristic DNA methylation differences have been identified between primary and metastatic melanomas at EBF3 and/or TBC1D16 gene loci. To further evaluate whether these epigenetic changes may act more generally as drivers of tumour onset and metastasis, we have investigated DNA methylation changes involv...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Rodger EJ,Chatterjee A,Stockwell PA,Eccles MR

    更新日期:2019-08-05 00:00:00

  • Bronchial biopsy specimen as a surrogate for DNA methylation analysis in inoperable lung cancer.

    abstract:Background:This study was aimed at understanding whether bronchial biopsy specimen can be used as a surrogate for DNA methylation analysis in surgically resected lung cancer. Methods:A genome-wide methylation was analyzed in 42 surgically resected tumor tissues, 136 bronchial washing, 12 sputum, and 8 bronchial biopsy...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Um SW,Kim HK,Kim Y,Lee BB,Kim D,Han J,Kim H,Shim YM,Kim DH

    更新日期:2017-12-20 00:00:00

  • DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer.

    abstract:BACKGROUND:Colorectal cancer (CRC) is the third most commonly diagnosed human malignancy worldwide. Upregulation of inhibitory immune checkpoints by tumor-infiltrating immune cells (TIICs) or their ligands by tumor cells leads to tumor evasion from host immunosurveillance. Changes in DNA methylation pattern and enrichm...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Sasidharan Nair V,Toor SM,Taha RZ,Shaath H,Elkord E

    更新日期:2018-08-06 00:00:00

  • The neuropeptide genes SST, TAC1, HCRT, NPY, and GAL are powerful epigenetic biomarkers in head and neck cancer: a site-specific analysis.

    abstract:Background:Staging and pathological grading systems are convenient but imperfect predictors of recurrence in head and neck squamous cell carcinoma (HNSCC). Identifying biomarkers for HNSCC that will progress and cause death is a critical research area, particularly if the biomarker can be linked to selection of patient...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Misawa K,Mima M,Imai A,Mochizuki D,Misawa Y,Endo S,Ishikawa R,Kanazawa T,Mineta H

    更新日期:2018-04-11 00:00:00

  • MUC1 is associated with TFF2 methylation in gastric cancer.

    abstract:BACKGROUND:Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1-/- mice compared with controls. However, little is known of the molecul...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Ge Y,Ma G,Liu H,Lin Y,Zhang G,Du M,Wang M,Chu H,Zhang H,Zhang Z

    更新日期:2020-03-02 00:00:00

  • Gene domain-specific DNA methylation episignatures highlight distinct molecular entities of ADNP syndrome.

    abstract:BACKGROUND:ADNP syndrome is a rare Mendelian disorder characterized by global developmental delay, intellectual disability, and autism. It is caused by truncating mutations in ADNP, which is involved in chromatin regulation. We hypothesized that the disruption of chromatin regulation might result in specific DNA methyl...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Bend EG,Aref-Eshghi E,Everman DB,Rogers RC,Cathey SS,Prijoles EJ,Lyons MJ,Davis H,Clarkson K,Gripp KW,Li D,Bhoj E,Zackai E,Mark P,Hakonarson H,Demmer LA,Levy MA,Kerkhof J,Stuart A,Rodenhiser D,Friez MJ,Stevenson

    更新日期:2019-04-27 00:00:00

  • Dynamic interplay between locus-specific DNA methylation and hydroxymethylation regulates distinct biological pathways in prostate carcinogenesis.

    abstract:BACKGROUND:Despite the significant global loss of DNA hydroxymethylation marks in prostate cancer tissues, the locus-specific role of hydroxymethylation in prostate tumorigenesis is unknown. We characterized hydroxymethylation and methylation marks by performing whole-genome next-generation sequencing in representative...

    journal_title:Clinical epigenetics

    pub_type: 杂志文章


    authors: Kamdar SN,Ho LT,Kron KJ,Isserlin R,van der Kwast T,Zlotta AR,Fleshner NE,Bader G,Bapat B

    更新日期:2016-03-15 00:00:00