Abstract:
BACKGROUND:This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice. METHODS:A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.2 mL PCV2 cells virulent strain SD (106.0 TCID50/mL) after 4 weeks. Average daily gain, blood antibody levels, microscopic changes and viremia were detected to estimate the effect of DNA vaccine. RESULTS AND DISCUSSION:The results showed that compared to those of the control mice, groups immunized with pVAX1-ORF2 and 18CpG-pVAX1-ORF2 could induce PCV2-specific antibodies. The PCV2-specific antibodies level of 18 CpG-pVAX1-ORF2 groups was higher significantly than other groups and decreased slowly along with time. There was no distinct pathological damage and viremia occurring in mice that inoculated with CpG motif DNA vaccines. The results demonstrated that the DNA vaccine containing 18 CpG could build up resistibility immunity and reduce immune organ damage on mice.
journal_name
Virol Jjournal_title
Virology journalauthors
Li J,Yu J,Xu S,Shi J,Xu S,Wu X,Fu F,Peng Z,Zhang L,Zheng S,Yuan X,Cong X,Sun W,Cheng K,Du Y,Wu J,Wang Jdoi
10.1186/s12985-016-0597-0subject
Has Abstractpub_date
2016-11-14 00:00:00pages
185issue
1issn
1743-422Xpii
10.1186/s12985-016-0597-0journal_volume
13pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Influenza virus continues to cause significant hospitalization rates in infants and young children. A 2-dose regime of trivalent inactivated vaccine is required to generate protective levels of hemagglutination inhibiting (HAI) antibodies. A vaccine preparation with enhanced immunogenicity is therefore desir...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-251
更新日期:2011-05-21 00:00:00
abstract::Adenovirus has a wide tissue tropism. The virus attaches to the surface of cells via the fiber protein knob binding to the Coxsackie and Adenovirus receptor known as CAR. Virus entry inside cells is facilitated by integrins αVβ3 and αVβ5. Mice platelets are shown to be the predominant Ad binding blood cell type and th...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-456
更新日期:2011-09-30 00:00:00
abstract:BACKGROUND:Human enterovirus (EV) and parechovirus (HPeV) are significant causes of encephalitis and meningitis in children. The aim of this study was to determine the prevalence, type and viral RNA concentration of EV and HPeV in cerebrospinal fluid (CSF) samples in an unselected cohort of patients <18 years admitted ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0427-9
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:To assess the potential effects of telbivudine (LdT) and entecavir (ETV) on renal function in patients with chronic hepatitis B (CHB), we performed a meta-analysis of the relevant data available on these agents to evaluate their effects on the estimated glomerular filtration rate (eGFR) during treatment. ME...
journal_title:Virology journal
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12985-016-0522-6
更新日期:2016-04-09 00:00:00
abstract::Most viruses take advantage of endocytic pathways to gain entry into host cells and initiate infections. Understanding of virus entry via endocytosis is critically important for the design of antiviral strategies. Virus entry via endocytosis is a complex process involving hundreds of cellular proteins. The entire proc...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-10-177
更新日期:2013-06-05 00:00:00
abstract::The original version of the acknowledgement unfortunately contained a mistake. ...
journal_title:Virology journal
pub_type: 已发布勘误
doi:10.1186/s12985-020-01439-2
更新日期:2020-11-09 00:00:00
abstract:BACKGROUND:The Capripoxvirus, Lumpy skin disease virus (LSDV) has a restricted host-range and is being investigated as a novel HIV-1 vaccine vector. LSDV does not complete its replication cycle in non-ruminant hosts. METHODS:The safety of LSDV was tested at doses of 104 and 106 plaque forming units in two strains of i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-265
更新日期:2011-05-30 00:00:00
abstract:BACKGROUND:Understanding how an organism replicates and assembles a multi-segmented genome with fidelity previously measured at 100% presents a model system for exploring questions involving genome assortment and RNA/protein interactions in general. The virus family Reoviridae, containing nine genera and more than 200 ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-1
更新日期:2006-01-03 00:00:00
abstract:BACKGROUND:The COVID-19 causing coronavirus is an enveloped RNA virus that utilizes an enzyme RNA dependent RNA polymerase for its replication. Favipiravir (FVP) triphosphate, a purine nucleoside analog, inhibits that enzyme. We have conducted this systematic review and meta-analysis on efficacy and safety of the drug ...
journal_title:Virology journal
pub_type: 杂志文章,meta分析
doi:10.1186/s12985-020-01412-z
更新日期:2020-09-24 00:00:00
abstract::Fatal influenza A virus infection is a major threat to public health throughout the world. Lung macrophages and neutrophils have critical roles for both the pathogenesis and viral clearance of fatal viral infections. These are complicated by the interaction of innate immunity and adaptive immunity against viral infect...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-57
更新日期:2014-03-26 00:00:00
abstract:BACKGROUND:The recently sequenced 218 kb genome of morphologically atypical Bacillus thuringiensis phage 0305phi8-36 exhibited only limited detectable homology to known bacteriophages. The only known relative of this phage is a string of phage-like genes called BtI1 in the chromosome of B. thuringiensis israelensis. Th...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-97
更新日期:2007-10-05 00:00:00
abstract::Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-139
更新日期:2011-03-25 00:00:00
abstract:BACKGROUND:Varicella (chickenpox) exhibits a characteristic epidemiological pattern which is associated with climate. In general, primary infections in tropical regions are comparatively less frequent among children than in temperate regions. This peculiarity regarding varicella-zoster virus (VZV) infection among certa...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-370
更新日期:2011-07-27 00:00:00
abstract:BACKGROUND:Panicum streak virus (PanSV; Family Geminiviridae; Genus Mastrevirus) is a close relative of Maize streak virus (MSV), the most serious viral threat to maize production in Africa. PanSV and MSV have the same leafhopper vector species, largely overlapping natural host ranges and similar geographical distribut...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-194
更新日期:2009-11-10 00:00:00
abstract:BACKGROUND:To understand the prevalence and characteristics of human cytomegalovirus (HCMV) infection in children in the Weifang area, and to provide information for its prevention and treatment. METHODS:A comprehensive survey was performed from 2009 to 2012 in 7582 children from birth to 6 years of age hospitalized i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-42
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Most of the non-B HIV-1 subtypes are predominant in Sub-Saharan Africa and India although they have been found worldwide. In the last decade, immigration from these areas has increased considerably in Spain. The objective of this study was to evaluate the prevalence of non-B subtypes circulating in a cohort ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-416
更新日期:2011-08-26 00:00:00
abstract:BACKGROUND:Otitis media (OM) affects ≥80% of children before the age of three. OM can arise following co-infection with influenza A virus (IAV) and the bacterium Streptococcus pneumoniae. We have previously shown that H3 IAV strains (such as Udorn/72) induced a higher rate of bacterial OM than H1 strains (such as PR8/3...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-128
更新日期:2013-04-23 00:00:00
abstract::Presumptive phage particles associated with Xylella fastidiosa strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from CsCl equilibrium centrifugation dens...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-75
更新日期:2008-06-06 00:00:00
abstract:BACKGROUND:Recently, a new subset of CD4(+)T helper cell termed Follicular helper T cells (Tfh), which play a pivotal role in B cell activation and differentiation in lymphoid structures, has been reported to participate in some certain autoimmune diseases. But whether Tfh cells are involved in the pathogenesis of VMC ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0257-9
更新日期:2015-02-11 00:00:00
abstract:BACKGROUND:Cervical cancer is a major public health problem worldwide. While Romania has the highest incidence of cervical cancer in Europe, the prevalence of HPV has not been evaluated. We report the first data on HPV prevalence and type distribution in Northeast Romania. METHODS:HPV prevalence and genotype distribut...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-558
更新日期:2011-12-22 00:00:00
abstract:BACKGROUND:Due to potential advantages, human adenoviral vectors have been evaluated pre-clinically as recombinant vaccine vectors against several cancers and infectious diseases, including human immunodeficiency virus (HIV) infection. The V3 loop of HIV-1 glycoprotein 120 (gp120) contains important neutralizing epitop...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-112
更新日期:2014-06-16 00:00:00
abstract:BACKGROUND:Longitudinal mark-recapture studies of rodents in two sites in the Mbaracayú Biosphere Reserve in the Interior Atlantic Forest of eastern Paraguay have revealed a complex and intriguing pattern of hantaviruses harbored by rodents in this area. Full-length sequencing and phylogenetic analyses were conducted f...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-399
更新日期:2011-08-12 00:00:00
abstract:BACKGROUND:Multi-resistant Achromobacter xylosoxidans has been recognized as an emerging pathogen causing nosocomially acquired infections during the last years. Phages as natural opponents could be an alternative to fight such infections. Bacteriophages against this opportunistic pathogen were isolated in a recent stu...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-14
更新日期:2014-01-27 00:00:00
abstract:BACKGROUND:Human cytomegalovirus (HCMV) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. HCMV immediate early (IE) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (ORF) UL82, and this transactivation activity...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-65
更新日期:2009-05-27 00:00:00
abstract::Porcine circoviruses are circular single-stranded DNA viruses that infect swine and wild boars. Two species of porcine circoviruses exist. Porcine circovirus type 1 is non pathogenic contrary to porcine circovirus type 2 which is associated with the disease known as Post-weaning Multisystemic Wasting Syndrome. Porcine...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-6-60
更新日期:2009-05-18 00:00:00
abstract:BACKGROUND:Rhabdoviridae is one of the most ecologically diverse families of RNA viruses which can infect a wide range of vertebrates and invertebrates. Bats, among mammals, are pointed to harbor a significantly higher proportion of unknown or emerging viruses with zoonotic potential. Herein, we report the isolation of...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-0949-z
更新日期:2018-02-17 00:00:00
abstract:BACKGROUND:Citrus sudden death (CSD), a disease that rapidly kills orange trees, is an emerging threat to the Brazilian citrus industry. Although the causal agent of CSD has not been definitively determined, based on the disease's distribution and symptomatology it is suspected that the agent may be a new strain of Cit...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-9
更新日期:2008-01-16 00:00:00
abstract::Members of the genus Cypovirus (family Reoviridae) are common pathogens of insects. These viruses have linear dsRNA genomes divided into 10-11 segments, which have generally been assumed to be monocistronic. Here, bioinformatic evidence is presented for a short overlapping coding sequence (CDS) in the cypovirus genome...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-62
更新日期:2008-05-20 00:00:00
abstract:BACKGROUND:During October 2010, Egypt reported an outbreak of acute hemorrhagic conjunctivitis (AHC). A total of 1831 cases were reported from three governorates; 1703 cases in El Daqahliya, 92 cases in Port Said, and 36 in Damietta. The purpose of this study was to identify and characterize the causative agent associa...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-96
更新日期:2013-03-25 00:00:00
abstract:BACKGROUND:Porcine circovirus type-2b (PCV2b) is recognized as the etiological agent of the various clinical manifestations of porcine circovirus-associated disease (PCVAD). Previous studies have demonstrated effectiveness of chimeric PCV1-2 vaccines against PCV2b challenge. In this study, the efficacy of inactivated a...
journal_title:Virology journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12985-015-0338-9
更新日期:2015-07-30 00:00:00