Rapid detection of anti-Vaccinia virus neutralizing antibodies.

Abstract:

:Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR.

journal_name

Virol J

journal_title

Virology journal

authors

Kramski M,Drozd A,Lichtfuss GF,Dabrowski PW,Ellerbrok H

doi

10.1186/1743-422X-8-139

subject

Has Abstract

pub_date

2011-03-25 00:00:00

pages

139

issn

1743-422X

pii

1743-422X-8-139

journal_volume

8

pub_type

杂志文章
  • Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan.

    abstract::Six major Hepatitis C virus (HCV) genotypes and hundreds of subtypes have been identified globally. All these genotypes are generally studied for epidemiology, their vaccine development and clinical management. This article comments the frequency distribution of various HCV genotypes circulate in different areas/distr...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-203

    authors: Ali A,Ahmed H,Idrees M

    更新日期:2010-08-26 00:00:00

  • Does the viral subtype influence the biennial cycle of respiratory syncytial virus?

    abstract:BACKGROUND:The epidemic pattern of respiratory syncytial virus (RSV) is quite different in regions of Europe (biennial epidemics in alternating cycles of approximately 9 and 15 months) than in the Western Hemisphere (annual epidemics). In order to determine if these differences are accounted for by the circulation of d...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-133

    authors: Mlinaric-Galinovic G,Vojnovic G,Cepin-Bogovic J,Bace A,Bozikov J,Welliver RC,Wahn U,Cebalo L

    更新日期:2009-09-07 00:00:00

  • Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70.

    abstract:BACKGROUND:Infectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, n...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0702-z

    authors: Zhang W,Cai C,Lin L,Tao YJ,Jin M

    更新日期:2017-02-15 00:00:00

  • Hepatitis B virus in Pakistan: a systematic review of prevalence, risk factors, awareness status and genotypes.

    abstract::In Pakistan, there are estimated 7-9 million carriers of hepatitis B virus (HBV) with a carrier rate of 3-5%. This article reviews the available literature about the prevalence, risk factors, awareness status and genotypes of the HBV in Pakistan by using key words; HBV prevalence, risk factors, awareness status and ge...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/1743-422X-8-102

    authors: Ali M,Idrees M,Ali L,Hussain A,Ur Rehman I,Saleem S,Afzal S,Butt S

    更新日期:2011-03-06 00:00:00

  • Interactions of viruses in Cowpea: effects on growth and yield parameters.

    abstract::The study was carried out to investigate the effects of inoculating three cowpea cultivars: "OLO II", "OLOYIN" and IT86D-719 with three unrelated viruses: Cowpea aphid-borne mosaic virus (CABMV), genus Potyvirus, Cowpea mottle virus (CMeV), genus Carmovirus and Southern bean mosaic virus (SBMV), genus Sobemovirus sing...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-15

    authors: Kareem KT,Taiwo MA

    更新日期:2007-02-08 00:00:00

  • Novel snake papillomavirus does not cluster with other non-mammalian papillomaviruses.

    abstract::Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals. We identified, cloned and sequenced PV DNA from pigmented papilloma-like lesions of a diamond python (Morelia spilota spilota). This represents the first ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-436

    authors: Lange CE,Favrot C,Ackermann M,Gull J,Vetsch E,Tobler K

    更新日期:2011-09-12 00:00:00

  • Hepatitis C virus NS2 and NS3/4A proteins are potent inhibitors of host cell cytokine/chemokine gene expression.

    abstract:BACKGROUND:Hepatitis C virus (HCV) encodes several proteins that interfere with the host cell antiviral response. Previously, the serine protease NS3/4A was shown to inhibit IFN-beta gene expression by blocking dsRNA-activated retinoic acid-inducible gene I (RIG-I) and Toll-like receptor 3 (TLR3)-mediated signaling pat...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-3-66

    authors: Kaukinen P,Sillanpää M,Kotenko S,Lin R,Hiscott J,Melén K,Julkunen I

    更新日期:2006-09-01 00:00:00

  • Drug susceptibility to etravirine and darunavir among Human Immunodeficiency Virus Type 1-derived pseudoviruses in treatment-experienced patients with HIV/AIDS in South Korea.

    abstract:BACKGROUND:In South Korea, about 20 types of antiretroviral drugs are used in the treatment of patients with human immunodeficiency virus/acquired immune deficiency syndrome. Since 2010, raltegravir, etravirine, and darunavir have been spotlighted as new drugs for highly active antiretroviral therapy (HAART)-experience...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0283-7

    authors: Kwon OK,Kim SS,Rhee JE,Kee MK,Park M,Oh HR,Choi JY

    更新日期:2015-04-09 00:00:00

  • Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression.

    abstract:BACKGROUND:Bovine leukemia virus (BLV), which is closely related to human T-cell leukemia virus, is the etiological agent of enzootic bovine leukosis, a disease characterized by a highly prolonged course involving persistent lymphocytosis and B-cell lymphoma. The bovine major histocompatibility complex class II region ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-019-1259-9

    authors: Bai L,Takeshima SN,Sato M,Davis WC,Wada S,Kohara J,Aida Y

    更新日期:2019-12-16 00:00:00

  • Oncolytic activity of a coxsackievirus B3 strain in human endometrial cancer cell lines.

    abstract:BACKGROUND:Endometrial cancer (EC) is one of the most common gynecological malignancies globally. Although progress has been made in surgical and other adjuvant therapies, there is still a great need to develop new approaches to further reduce the incidence and mortality of EC. Oncolytic virotherapy offers a novel prom...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-018-0975-x

    authors: Lin Y,Wang W,Wan J,Yang Y,Fu W,Pan D,Cai L,Cheng T,Huang X,Wang Y

    更新日期:2018-04-10 00:00:00

  • Cellular transcripts of chicken brain tissues in response to H5N1 and Newcastle disease virus infection.

    abstract:BACKGROUND:Highly-pathogenic avian influenza (HPAI) H5N1 and Newcastle disease (ND) viruses are the two most important poultry viruses in the world, with the ability to cause classic central nervous system dysfunction in poultry and migratory birds. To elucidate the mechanisms of neurovirulence caused by these viruses,...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-53

    authors: Balasubramaniam VR,Wai TH,Omar AR,Othman I,Hassan SS

    更新日期:2012-02-23 00:00:00

  • Study on suitable analysis method for HIV-1 non-catalytic integrase inhibitor.

    abstract:BACKGROUND:Integrase (IN) is an essential protein for HIV replication that catalyzes insertion of the reverse-transcribed viral genome into the host chromosome during the early steps of viral infection. Highly active anti-retroviral therapy is a HIV/AIDS treatment method that combines three or more antiviral drugs ofte...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01476-x

    authors: Park KH,Kim M,Bae SE,Lee HJ,Kim KC,Choi BS,Kim YB

    更新日期:2021-01-13 00:00:00

  • Potential effects of telbivudine and entecavir on renal function: a systematic review and meta-analysis.

    abstract:BACKGROUND:To assess the potential effects of telbivudine (LdT) and entecavir (ETV) on renal function in patients with chronic hepatitis B (CHB), we performed a meta-analysis of the relevant data available on these agents to evaluate their effects on the estimated glomerular filtration rate (eGFR) during treatment. ME...

    journal_title:Virology journal

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/s12985-016-0522-6

    authors: Wu X,Cai S,Li Z,Zheng C,Xue X,Zeng J,Peng J

    更新日期:2016-04-09 00:00:00

  • A new example of viral intein in Mimivirus.

    abstract:BACKGROUND:Inteins are "protein introns" that remove themselves from their host proteins through an autocatalytic protein-splicing. After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus. RESULTS:Here we report the identifi...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-2-8

    authors: Ogata H,Raoult D,Claverie JM

    更新日期:2005-02-11 00:00:00

  • Isolation of mixed subtypes of influenza A virus from a bald eagle (Haliaeetus leucocephalus).

    abstract::From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool con...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-174

    authors: Goyal SM,Jindal N,Chander Y,Ramakrishnan MA,Redig PT,Sreevatsan S

    更新日期:2010-07-28 00:00:00

  • Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids.

    abstract::Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, w...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-42

    authors: Vargas M,Martínez-García B,Díaz-Ruíz JR,Tenllado F

    更新日期:2008-03-19 00:00:00

  • Identification of two internal signal peptide sequences: critical for classical swine fever virus non-structural protein 2 to trans-localize to the endoplasmic reticulum.

    abstract:BACKGROUND:The membrane topology and molecular mechanisms for endoplasmic reticulum (ER) localization of classical swine fever virus (CSFV) non-structural 2 (NS2) protien is unclear. We attempted to elucidate the subcellular localization, and the molecular mechanisms responsible for the localization of this protein in ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-236

    authors: Guo KK,Tang QH,Zhang YM,Kang K,He L

    更新日期:2011-05-18 00:00:00

  • Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus.

    abstract::The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubili...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-50

    authors: Hsu HT,Tseng YH,Chou YL,Su SH,Hsu YH,Chang BY

    更新日期:2009-05-07 00:00:00

  • Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus.

    abstract:BACKGROUND:The major structural protein of coronaviruses, the membrane (M) protein, can elicit the formation of protective antibodies, but little information is available about the M protein of porcine epidemic diarrhea virus (PEDV). Identification of epitopes on the PEDV M protein will be helpful in the elucidation of...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-225

    authors: Zhang Z,Chen J,Shi H,Chen X,Shi D,Feng L,Yang B

    更新日期:2012-10-01 00:00:00

  • Geminivirus mixed infection on pepper plants: synergistic interaction between PHYVV and PepGMV.

    abstract:BACKGROUND:PHYVV and PepGMV are plant viruses reported in Mexico and Southern US as causal agents of an important pepper disease known as "rizado amarillo". Mixed infections with PHYVV and PepGMV have been reported in several hosts over a wide geographic area. Previous work suggested that these viruses might interact a...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-104

    authors: Rentería-Canett I,Xoconostle-Cázares B,Ruiz-Medrano R,Rivera-Bustamante RF

    更新日期:2011-03-08 00:00:00

  • Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone.

    abstract:BACKGROUND:Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to prevent chronic infection is important. We reported earlier that a re...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-118

    authors: Chandra PK,Hazari S,Poat B,Gunduz F,Prabhu R,Liu G,Burioni R,Clementi M,Garry RF,Dash S

    更新日期:2010-06-07 00:00:00

  • Rubella, herpes simplex virus type 2 and preeclampsia.

    abstract:BACKGROUND:Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE:The aim of the present study was to investigate the association between maternal seropositivity of rubella, He...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0813-6

    authors: Alshareef SA,Eltom AM,Nasr AM,Hamdan HZ,Adam I

    更新日期:2017-07-26 00:00:00

  • Capsid display of a conserved human papillomavirus L2 peptide in the adenovirus 5 hexon protein: a candidate prophylactic hpv vaccine approach.

    abstract:BACKGROUND:Infection by any one of 15 high risk human papillomavirus (hrHPV) types causes most invasive cervical cancers. Their oncogenic genome is encapsidated by L1 (major) and L2 (minor) coat proteins. Current HPV prophylactic vaccines are composed of L1 virus-like particles (VLP) that elicit type restricted immunit...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0364-7

    authors: Wu WH,Alkutkar T,Karanam B,Roden RB,Ketner G,Ibeanu OA

    更新日期:2015-09-11 00:00:00

  • Systemic virus distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virus.

    abstract:BACKGROUND:Avian influenza virus (AIV) is classified into two pathotypes, low pathogenic (LP) and high pathogenic (HP), based on virulence in chickens.Differences in pathogenicity between HPAIV and LPAIV might eventually be related to specific characteristics of strains, tissue tropism and host responses. METHODS:To s...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-61

    authors: Post J,Burt DW,Cornelissen JB,Broks V,van Zoelen D,Peeters B,Rebel JM

    更新日期:2012-03-06 00:00:00

  • Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens.

    abstract:BACKGROUND:In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0353-x

    authors: Susta L,Diel DG,Courtney S,Cardenas-Garcia S,Sundick RS,Miller PJ,Brown CC,Afonso CL

    更新日期:2015-08-08 00:00:00

  • Nuclear localized Influenza nucleoprotein N-terminal deletion mutant is deficient in functional vRNP formation.

    abstract:BACKGROUND:The influenza RNA dependent RNA polymerase synthesizes viral RNA in the nucleus as functional viral ribonucleoprotein (vRNP) complexes with RNA and nucleoprotein (NP). The N-terminus of NP contains an unconventional nuclear localization signal (NLS) important for initial vRNP nuclear localization but which a...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-155

    authors: Sanchez A,Guerrero-Juarez CF,Ramirez J,Newcomb LL

    更新日期:2014-08-31 00:00:00

  • The dual role of tetraspanin CD63 in HIV-1 replication.

    abstract:BACKGROUND:Previously, we showed that the tetraspanin membrane protein CD63 mediates both early and post-integration stages of the HIV-1 replication cycle. The temporal roles of CD63 were discerned using monoclonal antibodies and small interfering RNAs (siRNAs) to block CD63 function, and determining which of the seque...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-23

    authors: Li G,Endsley MA,Somasunderam A,Gbota SL,Mbaka MI,Murray JL,Ferguson MR

    更新日期:2014-02-08 00:00:00

  • A brief review on molecular, genetic and imaging techniques for HCV fibrosis evaluation.

    abstract:BACKGROUND:Chronic HCV is one of the major causes of morbidity and mortality in the present day world. The assessment of disease progression not only provides useful information for diagnosis and therapeutic supervision judgment but also for monitoring disease. Different invasive and non invasive methods are applied to...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/1743-422X-8-53

    authors: Ahmad W,Ijaz B,Gull S,Asad S,Khaliq S,Jahan S,Sarwar MT,Kausar H,Sumrin A,Shahid I,Hassan S

    更新日期:2011-02-08 00:00:00

  • Evaluation of nanoparticle tracking analysis for total virus particle determination.

    abstract::The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method's accuracy was demonstrated by using known...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-265

    authors: Kramberger P,Ciringer M,Štrancar A,Peterka M

    更新日期:2012-11-12 00:00:00

  • Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.

    abstract::Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral r...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-2-7

    authors: Radonić A,Thulke S,Bae HG,Müller MA,Siegert W,Nitsche A

    更新日期:2005-02-10 00:00:00