Abstract:
:Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
journal_name
Virol Jjournal_title
Virology journalauthors
Vargas M,Martínez-García B,Díaz-Ruíz JR,Tenllado Fdoi
10.1186/1743-422X-5-42subject
Has Abstractpub_date
2008-03-19 00:00:00pages
42issn
1743-422Xpii
1743-422X-5-42journal_volume
5pub_type
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