Abstract:
BACKGROUND:HIV-1 entry into cells is a multifaceted process involving target cell CD4 and the chemokine receptors, CXCR4 or CCR5. The lipid composition of the host cell plays a significant role in the HIV fusion process as it orchestrates the appropriate disposition of CD4 and co-receptors required for HIV-1 envelope glycoprotein (Env)-mediated fusion. The cell membrane is primarily composed of sphingolipids and cholesterol. The effects of lipid modulation on CD4 disposition in the membrane and their role in HIV-1 entry have extensively been studied. To focus on the role of lipid composition on chemokine receptor function, we have by-passed the CD4 requirement for HIV-1 Env-mediated fusion by using a CD4-independent strain of HIV-1 Env. RESULTS:Cell fusion mediated by a CD4-independent strain of HIV-1 Env was monitored by observing dye transfer between Env-expressing cells and NIH3T3 cells bearing CXCR4 or CCR5 in the presence or absence of CD4. Chemokine receptor signaling was assessed by monitoring changes in intracellular [Ca2+] mobilization induced by CCR5 or CXCR4 ligand. To modulate target membrane cholesterol or sphingolipids we used Methyl-beta-cyclodextrin (MbetaCD) or 1-phenyl-2-hexadecanoylamino-3-morpholino-1-propanol (PPMP), respectively. Treatment of the target cells with these agents did not change the levels of CD4 or CXCR4, but reduced levels of CCR5 on the cell surface. Chemokine receptor signalling was inhibited by cholesterol removal but not by treatment with PPMP. HIV-1 Env mediated fusion was inhibited by >50% by cholesterol removal. Overall, PPMP treatment appeared to slow down the rates of CD4-independent HIV-1 Env-mediated Fusion. However, in the case of CXCR4-dependent fusion, the differences between untreated and PPMP-treated cells did not appear to be significant. CONCLUSION:Although modulation of cholesterol and sphingolipids has similar effects on CD4-dependent HIV-1 Env-mediated fusion, sphingolipid modulation had little effect on CD4-independent HIV-1 Env-mediated fusion. Chemokine receptor function remained intact following treatment of cells with PPMP. Therefore such treatment may be considered a more suitable agent to inhibit CD4 dependent HIV-1 infection.
journal_name
Virol Jjournal_title
Virology journalauthors
Ablan S,Rawat SS,Viard M,Wang JM,Puri A,Blumenthal Rdoi
10.1186/1743-422X-3-104subject
Has Abstractpub_date
2006-12-22 00:00:00pages
104issn
1743-422Xpii
1743-422X-3-104journal_volume
3pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:The mechanisms through which the avian influenza virus H5N1 modulate the host's innate immune defense during invasion, remains incompletely understood. RIG-I as a pattern recognition receptor plays an important role in mediating innate immune response induced by influenza virus. So, modulating RIG-I might be...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-145
更新日期:2012-08-03 00:00:00
abstract::Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct l...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-165
更新日期:2009-10-12 00:00:00
abstract:BACKGROUND:The pattern recognition receptors (PPRs) are the earliest phase of the host defense against pathogens in genital epithelium, and toll-like receptors (TLRs) are best characterized PPRs mediating innate immune responses. Herpes simplex virus type 2 (HSV-2), a member of herpesviridae family, causes one of the m...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1087-3
更新日期:2018-11-12 00:00:00
abstract:BACKGROUND:Hepatitis C virus roots a chronic liver disease. Currently approved treatment strategy includes administration of alpha interferon and ribavirin combined therapy for 24-48 weeks. One of the predictor of sustained virological response is an early virological response to treatment characterized as rapid respon...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-253
更新日期:2011-05-23 00:00:00
abstract:BACKGROUND:Duck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected ducks is essential to control DuCV effectively. Therefore, this study aims to develop of an assay for DuCV to be highly specific, sensitive, and simple without a...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-76
更新日期:2014-04-29 00:00:00
abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01353-7
更新日期:2020-06-22 00:00:00
abstract::Intracellular influenza virus nucleoprotein (NP) is characterized by a high efficiency of homo-polymers formation, however their antigenic structure is still incompletely known. Herein, we report that RNase-resistant intracellular NP homo-polymers have a highly ordered conformational antigenic epitope, which depends o...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-37
更新日期:2008-02-29 00:00:00
abstract::Members of the genus Cypovirus (family Reoviridae) are common pathogens of insects. These viruses have linear dsRNA genomes divided into 10-11 segments, which have generally been assumed to be monocistronic. Here, bioinformatic evidence is presented for a short overlapping coding sequence (CDS) in the cypovirus genome...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-62
更新日期:2008-05-20 00:00:00
abstract:BACKGROUND:Nuclear factor-kappaB (NF-κB) is an inducible transcription factor that plays a key role in inflammation and immune responses, as well as in the regulation of cell proliferation and survival. Previous studies by our group and others have demonstrated that porcine reproductive and respiratory syndrome virus (...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-83
更新日期:2012-04-30 00:00:00
abstract:BACKGROUND:Subviral particles of hepatitis B virus (HBV) composed of L protein deletion variants with the 48 N-terminal amino acids of preS joined to the N-terminus of S protein (1-48preS/S) induced broadly neutralizing antibodies after immunization of mice with a Semliki Forest virus vector. A practical limitation for...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-63
更新日期:2013-02-25 00:00:00
abstract:BACKGROUND:The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. METHODS:Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. RESULTS:Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0701-0
更新日期:2017-02-08 00:00:00
abstract:BACKGROUND:Genome-wide RNAi screening has been widely used to identify host proteins involved in replication and infection of different viruses, and numerous host factors are implicated in the replication cycles of these viruses, demonstrating the power of this approach. However, discrepancies on target identification ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0420-3
更新日期:2015-11-24 00:00:00
abstract:BACKGROUND:Influenza viruses are characterized by their highly variable surface proteins HA and NA. The third surface protein M2 is a nearly invariant protein in all Influenza A strains. Despite extensive studies in other animal models, this study is the first to describe the use of recombinant M2 protein and a peptide...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-206
更新日期:2013-06-24 00:00:00
abstract:BACKGROUND:The human immunodeficiency virus type 1(HIV-1) is a complex retrovirus and the causative agent of acquired immunodeficiency syndrome (AIDS). The HIV-1 Vpu protein is an oligomeric integral membrane protein essential for particle release, viral load and CD4 degradation. In silico models show Vpu to form penta...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-81
更新日期:2007-08-29 00:00:00
abstract:BACKGROUND:Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifier hosts of JEV. Emerging evidence indicates that host microRNAs (miRNAs) play key roles in modulating viral infection and pathogenesis. However, mechanistic studies deli...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0562-y
更新日期:2016-06-21 00:00:00
abstract:BACKGROUND:For years, the understanding of the pathogenetic mechanisms that underlie honey bee viral diseases has been severely hindered because of the lack of a cell culture system for virus propagation. As a result, it is very imperative to develop new methods that would permit the in vitro pathogenesis study of hone...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-221
更新日期:2009-12-11 00:00:00
abstract:BACKGROUND:Both regulatory T cells (Tregs) and PD-1/PD-L1 pathway were critically involved in HCV viral persistence. However, the association between them was not well investigated. Herein, we aimed to investigate the distributional profiles of Tregs subsets and association between PD-1 expression on these subsets and ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-279
更新日期:2011-06-07 00:00:00
abstract:BACKGROUND:Jamestown Canyon virus (JCV), family Bunyaviridae, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-136
更新日期:2011-03-24 00:00:00
abstract:BACKGROUND:HIV-1 subtype B' isolates have been predominantly circulating in China. Their intra- and inter-subtype neutralization sensitivity to autologous and heterologous plasmas has not been well studied. RESULTS:Twelve HIV-1 B' clinical isolates obtained from patients were tested for their intra- and inter-subtype ...
journal_title:Virology journal
pub_type: 杂志文章,多中心研究
doi:10.1186/1743-422X-10-10
更新日期:2013-01-05 00:00:00
abstract:BACKGROUND:Norovirus is one of the most common causes of nonbacterial gastroenteritis in humans. Rapid spread by contaminated food and person-to-person transmission through the fecal-oral route are characteristics of norovirus epidemiology and result in high morbidity in vulnerable patient populations. Therefore, detec...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-260
更新日期:2011-05-26 00:00:00
abstract:BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-38
更新日期:2012-02-15 00:00:00
abstract:BACKGROUND:Classical swine fever (CSF) caused by virulent strains of Classical swine fever virus (CSFV) is a haemorrhagic disease of pigs, characterized by disseminated intravascular coagulation, thrombocytopoenia and immunosuppression, and the swine endothelial vascular cell is one of the CSFV target cells. In this re...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-4
更新日期:2010-01-11 00:00:00
abstract:BACKGROUND:The mint family (Lamiaceae) produces a wide variety of constituents with medicinal properties. Several family members have been reported to have antiviral activity, including lemon balm (Melissa officinalis L.), sage (Salvia spp.), peppermint (Mentha×piperita L.), hyssop (Hyssopus officinalis L.), basil (Oci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-188
更新日期:2011-04-23 00:00:00
abstract::Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-79
更新日期:2012-04-11 00:00:00
abstract:BACKGROUND:The aquatic birnavirus infectious pancreatic necrosis virus (IPNV) causes infectious pancreatic necrosis (IPN), a severe disease in farmed salmonid fish. IPNV has a very broad host range and infects many different species of fish as well as molluscs and crustaceans. Investigation of the host reservoir of a v...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-228
更新日期:2012-10-05 00:00:00
abstract:BACKGROUND:Persistent infection of human papillomavirus (HPV) types 16 and 18 causes cervical cancer. To better understand immune responses to the prophylactic vaccine, HPV 16/18 L1 virus-like particles (HPV-VLPs), we investigated B cell epitopes of HPV16 L1-derived peptides. METHODS:Sera from mice immunized with HPV-...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-199
更新日期:2012-09-14 00:00:00
abstract:BACKGROUND:Torquetenovirus (TTV) belongs to Anelloviridae family, infects nearly all people indefinitely without causing overt disease establishing a fine and successful interaction with the host. Increasing evidence have shown some human viruses exploit extracellular vesicles thereby helping viral persistence in the h...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1055-y
更新日期:2018-09-20 00:00:00
abstract:BACKGROUND:During the last two decades, structural biology analyses have shown that viruses infecting hosts far apart in evolution share similar architectural features, prompting a new virus classification based on structural lineages. Until recently, only a few prokaryotic viruses had been described for one of the lin...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-018-1097-1
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:Influenza A virus genomes are comprised of 8 negative strand single-stranded RNA segments and are thought to encode 11 proteins, which are all translated from mRNAs complementary to the genomic strands. Although human, swine and avian influenza A viruses are very similar, cross-species infections are usually...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-198
更新日期:2009-11-16 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV) causes significant morbidity and mortality globally primarily due to its ability to cause hepatitis, liver cirrhosis and hepatocellular carcinoma. The Kenya National Blood Transfusion Services screens for Hepatitis B antibodies using the chemiluminescent microparticle immunoassay meth...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01447-2
更新日期:2020-11-13 00:00:00