Abstract:
:Cell-derived microparticles (MPs) are nano-sized vesicles released by activated cells in the extracellular milieu. They act as vectors of biological activity by carrying membrane-anchored and cytoplasmic constituents of the parental cells. Although detection and characterization of cell-derived MPs may be of high diagnostic and prognostic values in a number of human diseases, reliable measurement of their size, number and biological activity still remains challenging using currently available methods. In the present study, we developed a protocol to directly image and functionally characterize MPs using high-resolution laser-scanning confocal microscopy. Once trapped on annexin-V coated micro-wells, we developed several assays using fluorescent reporters to measure their size, detect membrane antigens and evaluate proteolytic activity (nano-zymography). In particular, we demonstrated the applicability and specificity of this method to detect antigens and proteolytic activities of tissue-type plasminogen activator (tPA), urokinase and plasmin at the surface of engineered MPs from transfected cell-lines. Furthermore, we were able to identify a subset of tPA-bearing fibrinolytic MPs using plasma samples from a cohort of ischemic stroke patients who received thrombolytic therapy and in an experimental model of thrombin-induced ischemic stroke in mice. Overall, this method is promising for functional characterization of cell-derived MPs.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Briens A,Gauberti M,Parcq J,Montaner J,Vivien D,Martinez de Lizarrondo Sdoi
10.7150/thno.13757subject
Has Abstractpub_date
2016-03-02 00:00:00pages
610-26issue
5issn
1838-7640pii
thnov06p0610journal_volume
6pub_type
杂志文章相关文献
Theranostics文献大全abstract::Purpose: Colitis-associated colorectal cancer (CAC) poses substantial challenges for effective treatment. Currently, there is a considerable need for the development of orally bioavailable dosage forms that enable the safe and effective delivery of therapeutic drugs to local diseased lesions in the gastrointestinal tr...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.38081
更新日期:2019-10-12 00:00:00
abstract::Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous in their clinical behavior and require therapies specially tailored according to staging, grading, origin and expression of peptide receptors. Despite extensive scientific efforts, the therapy options are still not satisfactory. The main reasons are due...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.7851
更新日期:2014-01-29 00:00:00
abstract::Development of a rapid and sensitive method for Aβ(1-42) aggregation detection is of great importance to overcome the limitations of conventional techniques. In this study, we developed a label-free paper-based electrochemiluminescence sensor for amyloid-β aggregation detection toward potential diagnosis of Alzheimer'...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23483
更新日期:2018-03-11 00:00:00
abstract::Rationale: Corneal transplantation is an effective treatment to corneal blindness. However, the immune rejection imperils corneal allograft survival. An interventional modality is urgently needed to inhibit immune rejection and promote allograft survival. In our previous study, subconjunctival injections of bone marro...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31711
更新日期:2020-07-09 00:00:00
abstract::Rationale: Subjects unable to sustain β-cell compensation develop type 2 diabetes. Early growth response-1 protein (EGR-1), implicated in the regulation of cell differentiation, proliferation, and apoptosis, is induced by diverse metabolic challenges, such as glucose or other nutrients. Therefore, we hypothesized that...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.40664
更新日期:2020-03-04 00:00:00
abstract::Phenytoin, an antiepileptic drug, has been widely used for wound healing. Inspired by previous studies, phenytoin silver (PnAg), a sparingly soluble silver nanocompound, was synthesized which exhibited good therapeutic efficacy in tissue repair with low toxicity (LD50 >5 g/kg). In vivo studies showed that PnAg could a...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17073
更新日期:2017-01-05 00:00:00
abstract::Blood vessels are conduits distributed throughout the body, supporting tissue growth and homeostasis by the transport of cells, oxygen and nutrients. Endothelial cells (ECs) form the linings of the blood vessels, and together with pericytes, are essential for organ development and tissue homeostasis through producing ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.45422
更新日期:2020-05-01 00:00:00
abstract::Background: Among head and neck squamous cell carcinomas (HNSCCs), hypopharyngeal squamous cell carcinoma (HPSCC) has the worst prognosis. Iron metabolism, which plays a crucial role in tumor progression, is mainly regulated by alterations to genes and post-transcriptional processes. The recent discovery of the N6-met...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.51231
更新日期:2020-10-26 00:00:00
abstract::Near-infrared (NIR) fluorescence imaging has relied on fluorophores that emit in the 700-900 nm NIR-Ia or 1,000-1,700 nm NIR-II window for generating deep-tissue images. Up until now, there have been few fluorophores developed for the 900-1,000 nm NIR-Ib window. This is largely because NIR-Ib light is thought to be st...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26539
更新日期:2018-07-16 00:00:00
abstract::Background: Colorectal cancer (CRC) progression and related mortality are highly associated with metabolic disorders. However, the molecular mechanism involved in the regulation of hyperlipidemia-associated CRC metastasis remains unclear. This study aimed to investigate the effects of angiopoietin-like 4 (ANGPTL4) on ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44744
更新日期:2020-05-30 00:00:00
abstract::The structural modification of existing AMPs is an effective strategy to develop antimicrobial agents with high-efficiency, low-cost and low-toxicity antimicrobial agents. Methods: Here, we truncated 14-amino-acids at the N-terminus of MSI-78 to obtain MSI and further modified MSI to obtain four peptide analogs: MSI-1...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.39157
更新日期:2020-01-01 00:00:00
abstract::Targeted therapy and immunotherapy have become mainstream in cancer treatment. However, only patient subsets benefit from these expensive therapies, and often responses are short-lived or coincide with side effects. A growing modality in precision oncology is the development of theranostics, as this enables patient se...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.34941
更新日期:2019-10-15 00:00:00
abstract::To enhance effective drug accumulation in drug-resistant tumors, a site-specific drug-releasing polypeptide system (PEG-Phis/Pasp-DOX/CA4) was exploited in response to tumor extracellular and intracellular pH. This system could firstly release the embedded tumor vascular inhibitor (CA4) to transiently 'normalize' vasc...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.11821
更新日期:2015-04-28 00:00:00
abstract::RNAi-based strategies provide a great therapeutic potential for treatment of various human diseases including kidney disorders, but face the challenge of in vivo delivery and specific targeting. The chitosan delivery system has previously been shown to target siRNA specifically to the kidneys in mice when administered...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.7866
更新日期:2014-08-13 00:00:00
abstract::Purpose: Breast cancer imaging methods lack diagnostic accuracy, in particular for patients with dense breast tissue, and improved techniques are critically needed. The purpose of this study was to evaluate antibody-indocyanine green (ICG) conjugates, which undergo dynamic absorption spectrum shifts after cellular end...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18217
更新日期:2017-04-03 00:00:00
abstract::Incorporating a biomimetic coating and integrating osteoinductive biomolecules into basic bone substitutes are two common strategies to improve osteogenic capabilities in bone tissue engineering. Currently, the underlying mechanism of osteoporosis (OP)-related deficiency of osteogenesis remains unclear, and few treatm...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29566
更新日期:2019-01-30 00:00:00
abstract::Targeted radionuclide therapy, which is based on the selective delivery of a sufficient radiation dose to tumors without significantly affecting normal tissues, is a promising therapeutic approach for the treatment of a wide variety of malignancies. Integrins, a family of cell adhesion molecules, play key roles during...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno/v01p0201
更新日期:2011-03-01 00:00:00
abstract::Recently, researchers identified a distinct vessel subtype called type H vessels that couple angiogenesis and osteogenesis. We previously found that type H vessels are reduced in ovariectomy (OVX)-induced osteoporotic mice, and preosteoclasts are able to secrete platelet-derived growth factor-BB (PDGF-BB) to stimulate...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22144
更新日期:2018-03-28 00:00:00
abstract::Rationale: Tumor angiogenesis promotes tumor development, progression, growth, and metastasis. Metronomic chemotherapy involves the frequent administration of low-dose chemotherapeutic agents to block angiogenic activity and reduce side effects. Methods: MDA-MB-231 cells were treated with various concentrations of art...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33353
更新日期:2019-09-17 00:00:00
abstract::Zinc-finger proteins (ZFPs) are the largest transcription factor family in mammals, involved in the regulation of multiple physiologic processes including cell differentiation, proliferation, apoptosis and neoplastic transformation. Approximately one-third of ZFPs are Krüppel-associated box domain (KRAB)-ZFPs. Methods...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35861
更新日期:2020-01-12 00:00:00
abstract::Rationale: As the central hallmark of liver fibrosis, transdifferentiation of hepatic stellate cells (HSCs), the predominant contributor to fibrogenic hepatic myofibroblast responsible for extracellular matrix (ECM) deposition, is characterized with transcriptional and epigenetic remodeling. We aimed to characterize t...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.46360
更新日期:2021-01-01 00:00:00
abstract::Rationale: The overall success rate of prostate cancer (PCa) diagnosis and therapy has been improved over the years. However, genomic and phenotypic heterogeneity remains a major challenge for effective detection and treatment of PCa. Efforts to better classify PCa into functional subtypes and elucidate the molecular ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33292
更新日期:2019-05-24 00:00:00
abstract::Using non-covalent interactions between nucleic acids (DNA, siRNA, miRNA, and mRNA) with peptides or nucleopeptides is a promising strategy to construct supramolecular assemblies for gene delivery and therapy. Comparing to conventional strategies for gene delivery, the assemblies of peptides or nucleopeptides provide ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.31854
更新日期:2019-05-18 00:00:00
abstract::Intraoperative fluorescence imaging is particularly well-suited for surgical applications due to its inherently high sensitivity, resolution, and ability to provide images in real-time. To date, the intraoperative observation of fluorescence has largely been subjective. With the need to show objective evidence in orde...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.27384
更新日期:2018-10-22 00:00:00
abstract::During the last few decades, cell-based anti-tumor immunotherapy emerged and it has provided us with a large amount of knowledge. Upon chemokines recognition, immune cells undergo rapid trafficking and activation in disease milieu, with immune cells chemotaxis being accompanied by activation of diverse intercellular s...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.45219
更新日期:2020-05-15 00:00:00
abstract::Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48723
更新日期:2020-09-22 00:00:00
abstract::Pretargeting is a multi-step process that first has an unlabeled bispecific antibody (bsMAb) localize within a tumor by virtue of its anti-tumor binding site(s) before administering a small, fast-clearing radiolabeled compound that then attaches to the other portion of the bsMAb. The compound's rapid clearance signifi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3582
更新日期:2012-01-01 00:00:00
abstract::RNA molecules (e.g., siRNA, microRNA, and mRNA) have shown tremendous potential for immunomodulation and cancer immunotherapy. They can activate both innate and adaptive immune system responses by silencing or upregulating immune-relevant genes. In addition, mRNA-based vaccines have recently been actively pursued and ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.35568
更新日期:2020-01-01 00:00:00
abstract::Recently, self-assembling small dendrimers into supramolecular dendritic systems offers an alternative strategy to develop multifunctional nanoplatforms for biomedical applications. We herein report a dual-responsive supramolecular PEGylated dendritic system for efficient platinum-based drug delivery and near-infrared...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15081
更新日期:2016-06-05 00:00:00
abstract::Recent studies have highlighted the biological significance of RNA N6-methyladenosine (m6A) modification in tumorigenicity and progression. However, it remains unclear whether m6A modifications also have potential roles in immune regulation and tumor microenvironment (TME) formation. Methods: In this study, we curated...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.52717
更新日期:2021-01-01 00:00:00