Abstract:
:The structural modification of existing AMPs is an effective strategy to develop antimicrobial agents with high-efficiency, low-cost and low-toxicity antimicrobial agents. Methods: Here, we truncated 14-amino-acids at the N-terminus of MSI-78 to obtain MSI and further modified MSI to obtain four peptide analogs: MSI-1, MSI-2, MSI-3 and MSI-4. These peptide mutants were evaluated regarding their antibacterial activity against various sensitive or resistant bacteria; toxicity against mammalian cells or mice; and stability against violent pH, temperature variations and high NaCl concentrations. Finally, we also elucidated the possible mechanisms underlying its mode of action. Results: The results showed that MSI-1 and MSI-3 displayed activity that was superior to that of MSI-78 with MICs of 4-16 μg/ml and MBCs of 8-64 μg/ml, respectively, especially against drug-resistant bacteria, due to the increase in percent helicity and amphiphilicity. However, MSI-3, with higher hydrophobicity and antibacterial activity, had a relatively higher hemolysis rate and toxicity than MSI-1. MSI-1 exerted rapid bactericidal activity and effectively improved the survival rate and wound closure in penicillin-resistant E. coli-infected mice by eliminating bacterial counts in mouse organs or subeschar, further inhibiting the systemic dissemination of bacteria. Additionally, MSI-1 displayed perfect stability against violent pH, temperature variations and high NaCl concentrations and has the ability to circumvent the development of drug resistance. In terms of the mode of action, we found that at the super-MIC level, MSI-1 exhibited direct antimicrobial activity by disrupting the integrity of the bacterial cell membrane, while at the sub-MIC level, it bound to bacterial DNA to inhibit DNA replication and protein expression and ultimately disrupted bacterial biological function. Conclusions: This novel peptide MSI-1 could be a potential candidate for drug development against infection induced by drug-resistant bacteria.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Ma L,Xie X,Liu H,Huang Y,Wu H,Jiang M,Xu P,Ye X,Zhou Cdoi
10.7150/thno.39157subject
Has Abstractpub_date
2020-01-01 00:00:00pages
1373-1390issue
3issn
1838-7640pii
thnov10p1373journal_volume
10pub_type
杂志文章相关文献
Theranostics文献大全abstract::Sepsis is a major cause of patient mortality and morbidity from bacterial infections. Although neutrophils are known to be important in the development of sepsis, how distinctive neutrophil subtypes regulate inflammatory processes involved in septicemia remains unclear. Preconditioning protects organisms against subse...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.37139
更新日期:2019-08-14 00:00:00
abstract::Arachidonic acid (AA) is a polyunsaturated fatty acid present at high concentrations in the ovarian cancer (OC) microenvironment and associated with a poor clinical outcome. In the present study, we have unraveled a potential link between AA and macrophage functions. Methods: AA-triggered signal transduction was studi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.52442
更新日期:2021-01-01 00:00:00
abstract::Prostate-specific membrane antigen (PSMA) targeted PET has a high detection rate for biochemical recurrence (BCR) of prostate cancer (PCa). Nevertheless, even at high prostate-specific antigen (PSA) levels (> 3 ng/ml), a relevant number of PSMA-PET scans are negative, mainly due to PSMA-negative PCa. Our objective was...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44584
更新日期:2020-05-15 00:00:00
abstract::Bacteria-based anticancer therapies aim to overcome the limitations of current cancer therapy by actively targeting and efficiently removing cancer. To achieve this goal, new approaches that target and maintain bacterial drugs at sufficient concentrations during the therapeutic window are essential. Here, we examined ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16135
更新日期:2016-06-20 00:00:00
abstract::Biological cancer therapies, such as oncolytic, or replication-selective viruses have advantages over traditional therapeutics as they can employ multiple different mechanisms to target and destroy cancers (including direct cell lysis, immune activation and vascular collapse). This has led to their rapid recent clinic...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3724
更新日期:2012-01-01 00:00:00
abstract::Non-invasive PET imaging of CXCR4 expression in cancer and inflammation as well as CXCR4-targeted radioligand therapy (RLT) have recently found their way into clinical research by the development of the theranostic agents [68Ga]PentixaFor (cyclo(D-Tyr1-D-[NMe]Orn2(AMBS-[68Ga]DOTA)-Arg3-Nal4-Gly5) = [68Ga]DOTA-AMBS-CPC...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45537
更新日期:2020-07-09 00:00:00
abstract::This is the initial report of an α-based pre-targeted radioimmunotherapy (PRIT) using 225Ac and its theranostic pair, 111In. We call our novel tumor-targeting DOTA-hapten PRIT system "proteus-DOTA" or "Pr." Herein we report the first results of radiochemistry development, radiopharmacology, and stoichiometry of tumor ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48810
更新日期:2020-09-14 00:00:00
abstract::Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in var...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22952
更新日期:2018-02-07 00:00:00
abstract::Reactive oxygen species (ROS)-based photodynamic therapy (PDT) has a widespread application in cancer therapy. Nevertheless, the efficiency of PDT is far from satisfactory. One major impediment is the overexpression of glutathione (GSH) in tumor cells, which could deplete the level of PDT-generated ROS. Herein, we dev...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42633
更新日期:2020-04-15 00:00:00
abstract::Optical sensing plays an important role in theranostics due to its capability to detect hint biochemical entities or molecular targets as well as to precisely monitor specific fundamental psychological processes. Rare-earth (RE) doped upconversion nanoparticles (UCNPs) are promising for these endeavors due to their un...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.5305
更新日期:2013-03-26 00:00:00
abstract::Conventional immunosuppressants cause side effects and do not prevent the recurrence of autoimmune diseases. Moreover, they may not inhibit autoimmunity mediated by pathogenic memory T-cells. Dihydroartemisinin (DHA) has been shown to regulate autoimmunity. However, it remains unknown whether DHA impacts psoriasis and...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45211
更新日期:2020-08-21 00:00:00
abstract::The unique features of noble-metal nanostructures (NMNs) are leading to unprecedented expansion of research and exploration of their application in therapeutics, diagnostics and bioimaging fields. With the ever-growing applications of NMNs, both therapeutic and environmental NMNs are likely to be exposed to tissues an...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.42569
更新日期:2020-04-27 00:00:00
abstract:PURPOSE:The exact detection and delineation of the intraprostatic tumour burden is crucial for treatment planning in primary prostate cancer (PCa). We compared 68Ga-HBED-CC-PSMA PET/CT with multiparametric MRI (mpMRI) for diagnosis and tumour delineation in patients with primary PCa based on slice by slice correlation ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16638
更新日期:2017-01-01 00:00:00
abstract::Rationale: The incidence of hepatocellular carcinoma is rising worldwide. It is predicted that nearly half of the early-stage hepatocellular carcinoma (E-HCC) patients will develop recurrence. Dysregulated pH, a hallmark of E-HCC, is correlated with poor prognosis. The acidic microenvironment has been shown to promote...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30958
更新日期:2019-03-16 00:00:00
abstract::Protein therapeutics is playing an increasingly critical role in treatment of human diseases. However, current vectors are captured by the digestive endo-lysosomal system, which results in an extremely low fraction (<2%) of protein being released in the cytoplasm. This paper reports a drug-delivering-drug platform (HA...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23804
更新日期:2018-06-06 00:00:00
abstract::Rationale: Tumor microenvironment interacts with tumor cells to regulate their stemness properties through various cytokines and cytokine receptors. Previous studies revealed the possible role of interleukin 20 receptor subunit alpha (IL20RA) signaling in the progression of several types of tumors. However, its regula...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45280
更新日期:2021-01-01 00:00:00
abstract::Background: Tobacco smoke (TS) critically contributes to the development of lung cancer; however, the underlying molecular mechanisms remain unclear. The induction of cancer stem cells (CSCs) by TS represents an early event in tumor initiation. The lung cancer-related gene ΔNp63α is highly expressed in epithelial tiss...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33812
更新日期:2019-07-09 00:00:00
abstract::Developing ultrasensitive contrast agents for the early detection of malignant tumors in liver is highly demanded. Constructing hepatic tumors specific targeting probes could provide more sensitive imaging information but still faces great challenges. Here we report a novel approach for the synthesis of ultra-small Fe...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.14280
更新日期:2016-07-15 00:00:00
abstract::Background: Programmed cell death protein 1 (PD1) inhibitors have revolutionized cancer therapy, yet many patients fail to respond. Thus, the identification of accurate predictive biomarkers of therapy response will improve the clinical benefit of anti-PD1 therapy. Method: We assessed the baseline serological autoanti...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45816
更新日期:2020-05-16 00:00:00
abstract::CUB-domain containing protein 1 (CDCP1) is a cancer associated cell surface protein that amplifies pro-tumorigenic signalling by other receptors including EGFR and HER2. Its potential as a cancer target is supported by studies showing that anti-CDCP1 antibodies inhibit cell migration and survival in vitro, and tumor g...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30736
更新日期:2020-01-12 00:00:00
abstract::Intraoperative fluorescence imaging is particularly well-suited for surgical applications due to its inherently high sensitivity, resolution, and ability to provide images in real-time. To date, the intraoperative observation of fluorescence has largely been subjective. With the need to show objective evidence in orde...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.27384
更新日期:2018-10-22 00:00:00
abstract::Luminal androgen receptor (LAR) breast cancer accounts for 10% of all triple-negative breast cancers (TNBC). Anti-androgen therapy for this subtype is in development, but yields only partial clinical benefits. In this study, we aimed to characterize the genomic alterations of LAR TNBC, to analyze activation of the PI3...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.36182
更新日期:2020-01-01 00:00:00
abstract::Rationale: Triple-negative breast cancer (TNBC) is characterized by the absence of estrogen receptor alpha (ER-α), human epidermal growth factor receptor 2 (HER2) and progesterone receptor (PR) expression, but the effect of lacking the three factors on TNBC is unclear. Whether loss of the three factors contributes to ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30701
更新日期:2019-02-20 00:00:00
abstract::Many drug controlled release methods have been integrated in multifunctional nanoparticles, such as pH-, redox-, temperature-, enzyme-, and light-responsive release. However, few report is associated with the ROS responsive drug controlled release. Herein, a thioketal linker-based ROS responsive drug (camptothecin con...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.14101
更新日期:2016-02-05 00:00:00
abstract::Metastasis is a major cause of death in patients with colorectal cancer (CRC). Cysteine-rich protein 2 (CSRP2) has been recently implicated in the progression and metastasis of a variety of cancers. However, the biological functions and underlying mechanisms of CSRP2 in the regulation of CRC progression are largely un...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45674
更新日期:2020-09-02 00:00:00
abstract::Rationale: Peripheral nerve injury (PNI) is a great challenge for regenerative medicine. Nerve autograft is the gold standard for clinical PNI repair. Due to its significant drawbacks, artificial nerve guidance conduits (NGCs) have drawn much attention as replacement therapies. We developed a combinatorial NGC consist...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.50825
更新日期:2021-01-01 00:00:00
abstract::Tumor hypoxia is an important reason for the limited therapeutic efficacy of photodynamic therapy (PDT) because of the oxygen requirement of the therapeutic process. PDT consumes tissue oxygen and destroys tumor vasculature, further hampering its own efficacy in promoting tumor deterioration. Therefore, overcoming the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.36988
更新日期:2019-09-20 00:00:00
abstract::Gold nanoparticles (AuNPs) have been reported to promote osteogenic differentiation of mesenchymal stem cells and osteoblasts, but little is known about their effects on human periodontal ligament progenitor cells (PDLPs). In this study, we evaluated the effects of AuNPs with various diameters (5, 13 and 45 nm) on the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17252
更新日期:2017-03-06 00:00:00
abstract::High-dose radiotherapy effects are regulated by acute tumour endothelial cell death followed by rapid tumour cell death instead of canonical DNA break damage. Pre-treatment with ultrasound-stimulated microbubbles (USMB) has enabled higher-dose radiation effects with conventional radiation doses. This study aimed to co...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19010
更新日期:2018-01-01 00:00:00
abstract::This article aims at alerting scientists working with nanoparticles or microparticles about the specific adverse reactions due to the intravascular pulmonary macrophages present in pigs and other cloven-hoof animals, but not in humans. The history of a 25-year old study of an ultrasound contrast agent is used to illus...
journal_title:Theranostics
pub_type: 评论,杂志文章
doi:10.7150/thno.22420
更新日期:2017-10-23 00:00:00