Abstract:
:RNAi-based strategies provide a great therapeutic potential for treatment of various human diseases including kidney disorders, but face the challenge of in vivo delivery and specific targeting. The chitosan delivery system has previously been shown to target siRNA specifically to the kidneys in mice when administered intravenously. Here we confirm by 2D and 3D bioimaging that chitosan formulated siRNA is retained in the kidney for more than 48 hours where it accumulates in proximal tubule epithelial cells (PTECs), a process that was strongly dependent on the molecular weight of chitosan. Chitosan/siRNA nanoparticles, administered to chimeric mice with conditional knockout of the megalin gene, distributed almost exclusively in cells that expressed megalin, implying that the chitosan/siRNA particle uptake was mediated by a megalin-dependent endocytotic pathway. Knockdown of the water channel aquaporin 1 (AQP1) by up to 50% in PTECs was achieved utilizing the systemic i.v. delivery of chitosan/AQP1 siRNA in mice. In conclusion, specific targeting PTECs with the chitosan nanoparticle system may prove to be a useful strategy for knockdown of specific genes in PTECs, and provides a potential therapeutic strategy for treating various kidney diseases.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Gao S,Hein S,Dagnæs-Hansen F,Weyer K,Yang C,Nielsen R,Christensen EI,Fenton RA,Kjems Jdoi
10.7150/thno.7866subject
Has Abstractpub_date
2014-08-13 00:00:00pages
1039-51issue
10issn
1838-7640pii
thnov04p1039journal_volume
4pub_type
杂志文章相关文献
Theranostics文献大全abstract::Rationale: Chromodomain Y-like 2 (CDYL2) is a member of the CDY gene family involved in spermatogenesis, but its role in human cancer has not been reported. Analyses of publicly available databases demonstrate that CDYL2 is abundantly expressed in breast tumors. However, whether CDYL2 is involved in breast cancer prog...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.43744
更新日期:2020-04-06 00:00:00
abstract::Catalytic nanomaterials with intrinsic enzyme-like activities, called nanozymes, have recently attracted significant research interest due to their unique advantages relative to natural enzymes and conventional artificial enzymes. Among the nanozymes developed, particular interests have been devoted to nanozymes with ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19257
更新日期:2017-06-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in a variety of tumor cells, but not most normal cells. Nevertheless, its therapeutic potential is limited due to the frequent occurrence of resistance in tumor cells, especially hepatocellular carcinoma cell lines. Theref...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.21707
更新日期:2017-10-12 00:00:00
abstract::Stem cell tracking is a highly important subject. Current techniques based on nanoparticle-labeling, such as magnetic resonance imaging, fluorescence microscopy, and micro-computed tomography, are plagued by limitations including relatively low sensitivity or penetration depth, involvement of ionizing irradiation, and...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.5369
更新日期:2013-07-19 00:00:00
abstract::Smart drug delivery system with strategic drug distribution is the future state-of-the-art treatment for any malignancy. To investigate therapeutic potential of such nanoparticle mediated delivery system, we examined the efficacy of dual drug-loaded, pH and thermo liable lipid coated mesoporous iron oxide-based magnet...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15231
更新日期:2016-06-18 00:00:00
abstract::Ferroptosis is a newly discovered form of non-apoptotic cell death in multiple human diseases. However, the epigenetic mechanisms underlying ferroptosis remain poorly defined. First, we demonstrated that lymphoid-specific helicase (LSH), which is a DNA methylation modifier, interacted with WDR76 to inhibit ferroptosis...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19988
更新日期:2017-07-23 00:00:00
abstract::Acute liver failure is characterized by the rapid development of liver dysfunction and remarkably high mortality. Accumulating evidence suggests that soyasaponin possesses potential anti-inflammatory activities. Here, we aimed to investigate the potential role of soyasaponin II in acute liver failure and establish the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.40128
更新日期:2020-02-03 00:00:00
abstract::Rationale: Corneal transplantation is an effective treatment to corneal blindness. However, the immune rejection imperils corneal allograft survival. An interventional modality is urgently needed to inhibit immune rejection and promote allograft survival. In our previous study, subconjunctival injections of bone marro...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31711
更新日期:2020-07-09 00:00:00
abstract::Malignant glioma is one of the most untreatable cancers because of the formidable blood-brain barrier (BBB), through which few therapeutics can penetrate and reach the tumors. Biologics have been booming in cancer therapy in the past two decades, but their application in brain tumor has long been ignored due to the im...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.20578
更新日期:2017-08-15 00:00:00
abstract::Norepinephrine modulates cognitive processes such as working and episodic memory. Pathological changes in norepinephrine and norepinephrine transporter (NET) function and degeneration of the locus coeruleus produce irreversible impairments within the whole norepinephrine system, disrupting cognitive processes. Monitor...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29740
更新日期:2019-01-01 00:00:00
abstract::Model observers play an important role in the optimization and assessment of imaging devices. In this review paper, we first discuss the basic concepts of model observers, which include the mathematical foundations and psychophysical considerations in designing both optimal observers for optimizing imaging systems and...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.5138
更新日期:2013-10-04 00:00:00
abstract::Ocular neovascularization is a pathological sequel of multiple eye diseases. Based on the anatomical site into which the abnormal neovessels grow, ocular neovascularization can be categorized into corneal neovascularization, choroidal neovascularization, and retinal neovascularization. Each category is intractable, an...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.19646
更新日期:2017-07-22 00:00:00
abstract::Rationale: Tumor metastasis is the main cause for cancer-related death. However, the driving molecules of metastasis remain largely unknown. Here, we aim to identify long non-coding RNAs (lncRNAs) critical for human hepatocellular carcinoma (HCC) metastasis. Methods: Microarrays were used to screen a comprehensive set...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.32854
更新日期:2019-06-09 00:00:00
abstract::Cytotoxic T lymphocytes (CTLs) and their gene-engineered cells display great application prospects in tumor immunotherapy. The timing of CTL-induced molecular events in tumor cells is unclear, and we also unknow whether the killing efficiency of CTLs is restrained in the liver, an immunotolerant organ with a high tumo...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44979
更新日期:2021-01-01 00:00:00
abstract::Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48723
更新日期:2020-09-22 00:00:00
abstract::Rationale: Increasing frequency of human exposure to PEG-related products means that healthy people are likely to have pre-existing anti-PEG antibodies (pre-αPEG Ab). However, the influence of pre-αPEG Abs on the pharmacokinetics (PK) and therapeutic efficacy of LipoDox is unknown. Methods: We generated two pre-αPEG A...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22164
更新日期:2018-05-09 00:00:00
abstract::Great progress has been made in the field of tumor immunotherapy in the past decade. However, the therapeutic effects of immune checkpoint blockade (ICB) against ovarian cancer are still limited. Recently, an inhibitor of cyclin-dependent kinases 4 and 6 (CDK4/6i) has been reported to enhance antitumor immunity in pre...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44871
更新日期:2020-08-25 00:00:00
abstract::Systemic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) has become a promising strategy for disease treatment and tissue regeneration. Strategies to enhance the efficiency of BMSC cell therapy are crucial to promote its clinical application. Here, we aimed to improve BMSC cell therapy by inhibiting the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16911
更新日期:2017-01-01 00:00:00
abstract::Circulating tumor cells (CTCs) have shown promising potential as liquid biopsies that facilitate early detection, prognosis, therapeutic target selection and monitoring treatment response. CTCs in most cancer patients are low in abundance and heterogeneous in morphological and phenotypic profiles, which complicate the...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.18588
更新日期:2017-06-25 00:00:00
abstract::Rationale: Within the field of personalized medicine there is an increasing focus on designing flexible, multifunctional drug delivery systems that combine high efficacy with minimal side effects, by tailoring treatment to the individual. Methods: We synthesized a chemically stabilized ~4 nm nucleic acid nanoscaffold,...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.32060
更新日期:2019-04-13 00:00:00
abstract::Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expres...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23547
更新日期:2018-04-15 00:00:00
abstract::Chronic kidney disease (CKD) is a serious healthcare dilemma, associated with specific changes in gut microbiota and circulating metabolome. Yet, the functional capacity of CKD microbiome and its intricate relationship with the host metabolism at different stages of disease are less understood. METHODS:Here, shotgun ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.41725
更新日期:2020-04-06 00:00:00
abstract::Rationale: Busulfan is currently an indispensable anti-cancer drug, particularly for children, but the side effects on male reproduction are so serious that critical drug management is needed to minimize any negative impact. Meanwhile, alginate oligosaccharides (AOS) are natural products with many consequent advantage...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.43189
更新日期:2020-02-10 00:00:00
abstract::Rationale: Glioma stem-like cells (GSCs) contribute to temozolomide (TMZ) resistance in gliomas, although the mechanisms have not been delineated. Methods:In vitro functional experiments (colony formation assay, flow cytometric analysis, TUNEL assay) were used to assess the ability of extracellular vesicles (EVs) from...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47057
更新日期:2021-01-01 00:00:00
abstract::Salivary adenoid cystic carcinoma (SACC) is a peculiar malignant tumor, characterized by its slow but inexorable growth, with a high incidence of lung metastasis and poor prognosis. Here, we show the upregulated expression of EGFR ligand epiregulin in a subset of SACC cells correlates with lung metastasis and unfavora...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19712
更新日期:2017-08-23 00:00:00
abstract::Nanobubble (NB), which simultaneously enhances ultrasound (US) images and access therapeutic platforms, is required for future cancer treatment. Methods: We designed a theranostic agent for novel cancer treatment by using an NB-encapsulated hybrid nanosystem that can be monitored by US and fluorescent imaging and acti...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.38684
更新日期:2020-01-01 00:00:00
abstract::Background: Colorectal cancer (CRC) progression and related mortality are highly associated with metabolic disorders. However, the molecular mechanism involved in the regulation of hyperlipidemia-associated CRC metastasis remains unclear. This study aimed to investigate the effects of angiopoietin-like 4 (ANGPTL4) on ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44744
更新日期:2020-05-30 00:00:00
abstract::Intraoperative imaging with antibodies labeled with both a radionuclide for initial guidance and a near-infrared dye for adequate tumor delineation may overcome the main limitation of fluorescence imaging: the limited penetration depth of light in biological tissue. In this study, we demonstrate the feasibility and sa...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23335
更新日期:2018-03-08 00:00:00
abstract::The ability to selectively destroy cancer cells while sparing normal tissue is highly desirable during the cancer therapy. Here, magnetic targeted photothermal therapy was demonstrated by the integration of MoS2 (MS) flakes and Fe3O4 (IO) nanoparticles (NPs), where MoS2 converted near-infrared (NIR) light into heat an...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.11802
更新日期:2015-05-20 00:00:00
abstract::Talazoparib, a potent PARP inhibitor, has shown promising clinical and pre-clinical activity by inducing synthetic lethality in cancers with germline Brca1/2 mutations. Conventional oral delivery of Talazoparib is associated with significant off-target effects, therefore we sought to develop new delivery systems in th...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18563
更新日期:2017-09-26 00:00:00