Abstract:
:Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cross-reactive, non-neutralizing antibodies can enhance infection of Fc receptor bearing cells and, potentially, exacerbate disease. The actual binding sites of human antibody on the DENV particle are not well defined. We characterized the specificity and neutralization potency of polyclonal serum antibodies and memory B-cell derived monoclonal antibodies (hMAbs) from 2 individuals exposed to primary DENV infections. Most DENV-specific hMAbs were serotype cross-reactive and weakly neutralizing. Moreover, many hMAbs bound to the viral pre-membrane protein and other sites on the virus that were not preserved when the viral envelope protein was produced as a soluble, recombinant antigen (rE protein). Nonetheless, by modifying the screening procedure to detect rare antibodies that bound to rE, we were able to isolate and map human antibodies that strongly neutralized the homologous serotype of DENV. Our MAbs results indicate that, in these two individuals exposed to primary DENV infections, a small fraction of the total antibody response was responsible for virus neutralization.
journal_name
PLoS Negl Trop Disjournal_title
PLoS neglected tropical diseasesauthors
de Alwis R,Beltramello M,Messer WB,Sukupolvi-Petty S,Wahala WM,Kraus A,Olivarez NP,Pham Q,Brien JD,Tsai WY,Wang WK,Halstead S,Kliks S,Diamond MS,Baric R,Lanzavecchia A,Sallusto F,de Silva AMdoi
10.1371/journal.pntd.0001188subject
Has Abstractpub_date
2011-06-01 00:00:00pages
e1188issue
6eissn
1935-2727issn
1935-2735pii
PNTD-D-11-00001journal_volume
5pub_type
杂志文章abstract::Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU) disease, is unique amongst human pathogens in its capacity to produce a lipid toxin called mycolactone. While previous studies have demonstrated that bacterially-released mycolactone diffuses beyond infection foci, the spatiotemporal distribution of myco...
journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,评审
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journal_title:PLoS neglected tropical diseases
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doi:10.1371/journal.pntd.0002408
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journal_title:PLoS neglected tropical diseases
pub_type: 临床试验,杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,多中心研究
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0002556
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,meta分析,评审
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更新日期:2019-02-11 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2020-04-13 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pntd.0006212.]. ...
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,已发布勘误
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更新日期:2019-01-31 00:00:00
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