A chronic bioluminescent model of experimental visceral leishmaniasis for accelerating drug discovery.

Abstract:

BACKGROUND:Visceral leishmaniasis is a neglected parasitic disease with no vaccine available and its pharmacological treatment is reduced to a limited number of unsafe drugs. The scarce readiness of new antileishmanial drugs is even more alarming when relapses appear or the occurrence of hard-to-treat resistant strains is detected. In addition, there is a gap between the initial and late stages of drug development, which greatly delays the selection of leads for subsequent studies. METHODOLOGY/PRINCIPAL FINDINGS:In order to address these issues, we have generated a red-shifted luminescent Leishmania infantum strain that enables long-term monitoring of parasite burden in individual animals with an in vivo limit of detection of 106 intracellular amastigotes 48 h postinfection. For this purpose, we have injected intravenously different infective doses (104-5x108) of metacyclic parasites in susceptible mouse models and the disease was monitored from initial times to 21 weeks postinfection. The emission of light from the target organs demonstrated the sequential parasite colonization of liver, spleen and bone marrow. When miltefosine was used as proof-of-concept, spleen weight parasite burden and bioluminescence values decreased significantly. CONCLUSIONS:In vivo bioimaging using a red-shifted modified Leishmania infantum strain allows the appraisal of acute and chronic stage of infection, being a powerful tool for accelerating drug development against visceral leishmaniasis during both stages and helping to bridge the gap between early discovery process and subsequent drug development.

journal_name

PLoS Negl Trop Dis

authors

Álvarez-Velilla R,Gutiérrez-Corbo MDC,Punzón C,Pérez-Pertejo MY,Balaña-Fouce R,Fresno M,Reguera RM

doi

10.1371/journal.pntd.0007133

subject

Has Abstract

pub_date

2019-02-14 00:00:00

pages

e0007133

issue

2

eissn

1935-2727

issn

1935-2735

pii

PNTD-D-18-00984

journal_volume

13

pub_type

杂志文章
  • Evaluation of ocular involvement in patients with Hansen's disease.

    abstract::Hansen's disease (HD) belongs to the group of neglected diseases and can cause physical deformities and disabilities, in addition to leading to social discrimination. Ocular involvement in HD is estimated at 70-75% worldwide. About 10-50% suffer from severe ocular symptoms and loss of vision occurs in approximately 5%...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008585

    authors: Pavezzi PD,do Prado RB,Boin Filho PÂ,Gon ADS,Tuma B,Fornazieri MA,Scalone FM,Alves LRM,Montero RH,Casella AMB

    更新日期:2020-09-21 00:00:00

  • IL-33/ST2 correlates with severity of haemorrhagic fever with renal syndrome and regulates the inflammatory response in Hantaan virus-infected endothelial cells.

    abstract:BACKGROUND:Hantaan virus (HTNV) causes a severe lethal haemorrhagic fever with renal syndrome (HFRS) in humans. Despite a limited understanding of the pathogenesis of HFRS, the importance of the abundant production of pro-inflammatory cytokines has been widely recognized. Interleukin 33 (IL-33) has been demonstrated to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003514

    authors: Zhang Y,Zhang C,Zhuang R,Ma Y,Zhang Y,Yi J,Yang A,Jin B

    更新日期:2015-02-06 00:00:00

  • Using detergent-enhanced LAMP for African trypanosome detection in human cerebrospinal fluid and implications for disease staging.

    abstract:OBJECTIVE:Where human African trypanosomiasis (HAT) patients are seen, failure to microscopically diagnose infections by Trypanosoma brucei gambiense in blood smears and/or cerebrospinal fluid (CSF) in the critical early stages of the disease is the single most important factor in treatment failure, a result of delayed...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007631

    authors: Grab DJ,Nikolskaia OV,Courtioux B,Thekisoe OMM,Magez S,Bogorad M,Dumler JS,Bisser S

    更新日期:2019-08-19 00:00:00

  • Use of genomic DNA as an indirect reference for identifying gender-associated transcripts in morphologically identical, but chromosomally distinct, Schistosoma mansoni cercariae.

    abstract:BACKGROUND:The use of DNA microarray technology to study global Schistosoma gene expression has led to the rapid identification of novel biological processes, pathways or associations. Implementation of standardized DNA microarray protocols across laboratories would assist maximal interpretation of generated datasets a...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000323

    authors: Fitzpatrick JM,Protasio AV,McArdle AJ,Williams GA,Johnston DA,Hoffmann KF

    更新日期:2008-01-01 00:00:00

  • Thalidomide in the treatment of erythema nodosum leprosum (ENL) in an outpatient setting: A five-year retrospective analysis from a leprosy referral centre in India.

    abstract::Erythema nodosum leprosum (ENL), or type 2 lepra reaction, is a multi-system immune-mediated complication in patients with multibacillary leprosy, frequently associated with chronicity and recurrences. Management of ENL requires high doses of oral corticosteroids, which may not be universally effective and pose seriou...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008678

    authors: Upputuri B,Pallapati MS,Tarwater P,Srikantam A

    更新日期:2020-10-09 00:00:00

  • Access to Chagas disease treatment in the United States after the regulatory approval of benznidazole.

    abstract::Approximately 300,000 persons in the United States (US) are infected with Trypanosoma cruzi, the protozoan that causes Chagas disease, but less than 1% are estimated to have received antiparasitic treatment. Benznidazole was approved by the US Food and Drug Administration (FDA) for treatment of T. cruzi infection in 2...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008398

    authors: Yoshioka K,Manne-Goehler J,Maguire JH,Reich MR

    更新日期:2020-06-22 00:00:00

  • Spatio-temporal dynamics of dengue in Brazil: Seasonal travelling waves and determinants of regional synchrony.

    abstract::Dengue continues to be the most important vector-borne viral disease globally and in Brazil, where more than 1.4 million cases and over 500 deaths were reported in 2016. Mosquito control programmes and other interventions have not stopped the alarming trend of increasingly large epidemics in the past few years. Here, ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007012

    authors: Churakov M,Villabona-Arenas CJ,Kraemer MUG,Salje H,Cauchemez S

    更新日期:2019-04-22 00:00:00

  • Comparative Ability of Oropsylla montana and Xenopsylla cheopis Fleas to Transmit Yersinia pestis by Two Different Mechanisms.

    abstract:BACKGROUND:Transmission of Yersinia pestis by flea bite can occur by two mechanisms. After taking a blood meal from a bacteremic mammal, fleas have the potential to transmit the very next time they feed. This early-phase transmission resembles mechanical transmission in some respects, but the mechanism is unknown. Ther...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005276

    authors: Hinnebusch BJ,Bland DM,Bosio CF,Jarrett CO

    更新日期:2017-01-12 00:00:00

  • Antagonistic effects of Plasmodium-helminth co-infections on malaria pathology in different population groups in Côte d'Ivoire.

    abstract:INTRODUCTION:Plasmodium spp. and helminths are co-endemic in many parts of the tropics; hence, co-infection is a common phenomenon. Interactions between Plasmodium and helminth infections may alter the host's immune response and susceptibility and thus impact on morbidity. There is little information on the direction a...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007086

    authors: Hürlimann E,Houngbedji CA,Yapi RB,N'Dri PB,Silué KD,Ouattara M,Utzinger J,N'Goran EK,Raso G

    更新日期:2019-01-10 00:00:00

  • The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from Echinococcus granulosus sensu lato and protect mice against secondary cystic echinococcosis.

    abstract:BACKGROUND:Scavenger Receptors (SRs) from the host's innate immune system are known to bind multiple ligands to promote the removal of non-self or altered-self targets. CD5 and CD6 are two highly homologous class I SRs mainly expressed on all T cells and the B1a cell subset, and involved in the fine tuning of activatio...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006891

    authors: Mourglia-Ettlin G,Miles S,Velasco-De-Andrés M,Armiger-Borràs N,Cucher M,Dematteis S,Lozano F

    更新日期:2018-11-30 00:00:00

  • The concentrations of ambient Burkholderia pseudomallei during typhoon season in endemic area of melioidosis in Taiwan.

    abstract:BACKGROUND:Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei with a high case-fatality rate. Epidemiological and animal studies show the possibility of inhalation transmission. However, no B. pseudomallei concentrations in ambient air have been researched. Here, we developed a method to qu...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002877

    authors: Chen YL,Yen YC,Yang CY,Lee MS,Ho CK,Mena KD,Wang PY,Chen PS

    更新日期:2014-05-29 00:00:00

  • Vector competence of Aedes aegypti, Culex tarsalis, and Culex quinquefasciatus from California for Zika virus.

    abstract::Zika virus (ZIKV) has emerged since 2013 as a significant global human health threat following outbreaks in the Pacific Islands and rapid spread throughout South and Central America. Severe congenital and neurological sequelae have been linked to ZIKV infections. Assessing the ability of common mosquito species to tra...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006524

    authors: Main BJ,Nicholson J,Winokur OC,Steiner C,Riemersma KK,Stuart J,Takeshita R,Krasnec M,Barker CM,Coffey LL

    更新日期:2018-06-21 00:00:00

  • Spatiotemporal analysis of mycolactone distribution in vivo reveals partial diffusion in the central nervous system.

    abstract::Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU) disease, is unique amongst human pathogens in its capacity to produce a lipid toxin called mycolactone. While previous studies have demonstrated that bacterially-released mycolactone diffuses beyond infection foci, the spatiotemporal distribution of myco...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008878

    authors: Colucci-Guyon E,Rifflet A,Saint-Auret S,da Costa A,Boucontet L,Laval T,Prehaud C,Blanchard N,Levraud JP,Boneca IG,Demangel C,Guenin-Macé L

    更新日期:2020-12-02 00:00:00

  • Evaluation of methods for detection of asymptomatic individuals infected with Leishmania infantum in the state of Piauí, Brazil.

    abstract:BACKGROUND:Visceral Leishmaniasis in humans presents with fever, anemia, and splenomegaly and can be lethal if not treated. Nevertheless, the majority of Leishmania infantum-infected individuals does not manifest symptoms and remain so provided they are not immunosuppressed. In this work, the performance of different t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007493

    authors: Porcino GN,Carvalho KSS,Braz DC,Costa Silva V,Costa CHN,de Miranda Santos IKF

    更新日期:2019-07-01 00:00:00

  • Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.

    abstract:BACKGROUND:The liver fluke Opisthorchis viverrini is classified as a class I carcinogen due to the association between cholangiocarcinoma and chronic O. viverrini infection. During its feeding activity within the bile duct, the parasite secretes several cathepsin F cysteine proteases that may induce or contribute to th...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000398

    authors: Pinlaor P,Kaewpitoon N,Laha T,Sripa B,Kaewkes S,Morales ME,Mann VH,Parriott SK,Suttiprapa S,Robinson MW,To J,Dalton JP,Loukas A,Brindley PJ

    更新日期:2009-01-01 00:00:00

  • Rabies-induced behavioural changes are key to rabies persistence in dog populations: Investigation using a network-based model.

    abstract::Canine rabies was endemic pre-urbanisation, yet little is known about how it persists in small populations of dogs typically seen in rural and remote regions. By simulating rabies outbreaks in such populations (50-90 dogs) using a network-based model, our objective was to determine if rabies-induced behavioural change...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007739

    authors: Brookes VJ,Dürr S,Ward MP

    更新日期:2019-09-23 00:00:00

  • Autochthonous Chikungunya Transmission and Extreme Climate Events in Southern France.

    abstract:BACKGROUND:Extreme precipitation events are increasing as a result of ongoing global warming, but controversy surrounds the relationship between flooding and mosquito-borne diseases. A common view among the scientific community and public health officers is that heavy rainfalls have a flushing effect on breeding sites,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003854

    authors: Roiz D,Boussès P,Simard F,Paupy C,Fontenille D

    更新日期:2015-06-16 00:00:00

  • Telacebec (Q203)-containing intermittent oral regimens sterilized mice infected with Mycobacterium ulcerans after only 16 doses.

    abstract::Buruli ulcer (BU), caused by Mycobacterium ulcerans, is currently treated with a daily combination of rifampin and either injectable streptomycin or oral clarithromycin. An intermittent oral regimen would facilitate treatment supervision. We first evaluated the bactericidal activity of newer antimicrobials against M. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007857

    authors: Chauffour A,Robert J,Veziris N,Aubry A,Pethe K,Jarlier V

    更新日期:2020-08-31 00:00:00

  • A 23-year retrospective investigation of Salmonella Typhi and Salmonella Paratyphi isolated in a tertiary Kathmandu hospital.

    abstract:BACKGROUND:Salmonella serovars Typhi (S. Typhi) and Paratyphi A (S. Paratyphi A), the causative agents of enteric fever, have been routinely isolated organisms from the blood of febrile patients in the Kathmandu Valley since the early 1990s. Susceptibility against commonly used antimicrobials for treating enteric fever...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006051

    authors: Zellweger RM,Basnyat B,Shrestha P,Prajapati KG,Dongol S,Sharma PK,Koirala S,Darton TC,Dolecek C,Thompson CN,Thwaites GE,Baker SG,Karkey A

    更新日期:2017-11-27 00:00:00

  • Emergence of Rare Species of Nontuberculous Mycobacteria as Potential Pathogens in Saudi Arabian Clinical Setting.

    abstract:BACKGROUND:Clinical relevance of nontuberculous mycobacteria (NTM) is increasing worldwide including in Saudi Arabia. A high species diversity of NTM's has been noticed in a recent study. However, the identification in diagnostic laboratories is mostly limited to common species. The impact of NTM species diversity on c...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005288

    authors: Varghese B,Enani M,Shoukri M,AlThawadi S,AlJohani S,Al-Hajoj S

    更新日期:2017-01-11 00:00:00

  • High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.

    abstract:BACKGROUND:Protozoan parasites of the genus Leishmania cause a large spectrum of clinical manifestations known as Leishmaniases. These diseases are increasingly important public health problems in many countries both within and outside endemic regions. Thus, an accurate differential diagnosis is extremely relevant for ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004485

    authors: Zampieri RA,Laranjeira-Silva MF,Muxel SM,Stocco de Lima AC,Shaw JJ,Floeter-Winter LM

    更新日期:2016-02-29 00:00:00

  • Spatio-temporal factors associated with meningococcal meningitis annual incidence at the health centre level in Niger, 2004-2010.

    abstract:BACKGROUND:Epidemics of meningococcal meningitis (MM) recurrently strike the African Meningitis Belt. This study aimed at investigating factors, still poorly understood, that influence annual incidence of MM serogroup A, the main etiologic agent over 2004-2010, at a fine spatial scale in Niger. METHODOLOGY/PRINCIPAL F...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002899

    authors: Paireau J,Maïnassara HB,Jusot JF,Collard JM,Idi I,Moulia-Pelat JP,Mueller JE,Fontanet A

    更新日期:2014-05-22 00:00:00

  • Detection of venom after antivenom is not associated with persistent coagulopathy in a prospective cohort of Russell's viper (Daboia russelii) envenomings.

    abstract:BACKGROUND:Venom recurrence or persistence in the circulation after antivenom treatment has been documented many times in viper envenoming. However, it has not been associated with clinical recurrence for many snakes, including Russell's viper (Daboia spp.). We compare the recovery of coagulopathy to the recurrence or ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003304

    authors: Maduwage K,O'Leary MA,Scorgie FE,Shahmy S,Mohamed F,Abeysinghe C,Karunathilake H,Lincz LF,Gnanathasan CA,Isbister GK

    更新日期:2014-12-18 00:00:00

  • The associations between water and sanitation and hookworm infection using cross-sectional data from Togo's national deworming program.

    abstract:BACKGROUND:Sustainable control of soil-transmitted helminths requires a combination of chemotherapy treatment and environmental interventions, including access to safe drinking water, sufficient water for hygiene, use of clean sanitation facilities, and handwashing (WASH). We quantified associations between home-, scho...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006374

    authors: Baker JM,Trinies V,Bronzan RN,Dorkenoo AM,Garn JV,Sognikin S,Freeman MC

    更新日期:2018-03-28 00:00:00

  • Rapid mapping of urinary schistosomiasis: An appraisal of the diagnostic efficacy of some questionnaire-based indices among high school students in Katsina State, northwestern Nigeria.

    abstract:BACKGROUND:In sub-Saharan Africa, over 200 million individuals are estimated to be infected with urinary and intestinal schistosomiasis. In a bid to lay a foundation for effective future control programme, this study was carried out with the aim of assessing the diagnostic efficacy of some questionnaire-based rapid ass...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005518

    authors: Atalabi TE,Adubi TO,Lawal U

    更新日期:2017-04-03 00:00:00

  • Mechanisms of pyrethroid resistance in the dengue mosquito vector, Aedes aegypti: target site insensitivity, penetration, and metabolism.

    abstract::Aedes aegypti is the major vector of yellow and dengue fevers. After 10 generations of adult selection, an A. aegypti strain (SP) developed 1650-fold resistance to permethrin, which is one of the most widely used pyrethroid insecticides for mosquito control. SP larvae also developed 8790-fold resistance following sele...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002948

    authors: Kasai S,Komagata O,Itokawa K,Shono T,Ng LC,Kobayashi M,Tomita T

    更新日期:2014-06-19 00:00:00

  • Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products.

    abstract:INTRODUCTION:While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different lif...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000326

    authors: Moreno Y,Geary TG

    更新日期:2008-01-01 00:00:00

  • Inward rectifier potassium (Kir) channels mediate salivary gland function and blood feeding in the lone star tick, Amblyomma americanum.

    abstract:BACKGROUND:Tick feeding causes extreme morbidity and mortality to humans through transmission of pathogens and causes severe economic losses to the agricultural industry by reducing livestock yield. Salivary gland secretions are essential for tick feeding and thus, reducing or preventing saliva secretions into the vert...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007153

    authors: Li Z,Macaluso KR,Foil LD,Swale DR

    更新日期:2019-02-07 00:00:00

  • Biodistribution of degradable polyanhydride particles in Aedes aegypti tissues.

    abstract::Insecticide resistance poses a significant threat to the control of arthropods that transmit disease agents. Nanoparticle carriers offer exciting opportunities to expand the armamentarium of insecticides available for public health and other pests. Most chemical insecticides are delivered by contact or feeding, and fr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008365

    authors: Norris EJ,Mullis AS,Phanse Y,Narasimhan B,Coats JR,Bartholomay LC

    更新日期:2020-09-08 00:00:00

  • Tissue- and sex-specific lipidomic analysis of Schistosoma mansoni using high-resolution atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging.

    abstract::Schistosomes are human pathogens causing the neglected tropical disease schistosomiasis, which occurs worldwide in (sub-)tropical regions. This infectious disease is often associated with poverty, and more than 700 million people are at risk of infection. Exploitation of novel habitats and limited therapeutic options ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008145

    authors: Kadesch P,Quack T,Gerbig S,Grevelding CG,Spengler B

    更新日期:2020-05-13 00:00:00