Abstract:
BACKGROUND:A number of gene therapy applications would benefit from vectors capable of expressing multiple genes. In this study we explored the feasibility and efficiency of expressing two or three transgenes in HIV-1 based lentiviral vector. Bicistronic and tricistronic self-inactivating lentiviral vectors were constructed employing the internal ribosomal entry site (IRES) sequence of encephalomyocarditis virus (EMCV) and/or foot-and-mouth disease virus (FMDV) cleavage factor 2A. We employed enhanced green fluorescent protein (eGFP), O6-methylguanine-DNA-methyltransferase (MGMT), and homeobox transcription factor HOXB4 as model genes and their expression was detected by appropriate methods including fluorescence microscopy, flow cytometry, immunocytochemistry, biochemical assay, and western blotting. RESULTS:All the multigene vectors produced high titer virus and were able to simultaneously express two or three transgenes in transduced cells. However, the level of expression of individual transgenes varied depending on: the transgene itself; its position within the construct; the total number of transgenes expressed; the strategy used for multigene expression and the average copy number of pro-viral insertions. Notably, at limiting MOI, the expression of eGFP in a bicistronic vector based on 2A was approximately 4 times greater than that of an IRES based vector. CONCLUSION:The small and efficient 2A sequence can be used alone or in combination with an IRES for the construction of multicistronic lentiviral vectors which can express encoded transgenes at functionally relevant levels in cells containing an average of one pro-viral insert.
journal_name
Virol Jjournal_title
Virology journalauthors
Chinnasamy D,Milsom MD,Shaffer J,Neuenfeldt J,Shaaban AF,Margison GP,Fairbairn LJ,Chinnasamy Ndoi
10.1186/1743-422X-3-14subject
Has Abstractpub_date
2006-03-15 00:00:00pages
14issn
1743-422Xpii
1743-422X-3-14journal_volume
3pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Crimean-Congo Hemorrhagic Fever virus (CCHFV), a member of the genus Nairovirus, family Bunyaviridae, is a tick-borne pathogen causing severe disease in humans. To better understand the CCHFV life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-42
更新日期:2005-04-25 00:00:00
abstract:BACKGROUND:Chronic HCV is one of the major causes of morbidity and mortality in the present day world. The assessment of disease progression not only provides useful information for diagnosis and therapeutic supervision judgment but also for monitoring disease. Different invasive and non invasive methods are applied to...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-8-53
更新日期:2011-02-08 00:00:00
abstract:BACKGROUND:Influenza virus continues to cause significant hospitalization rates in infants and young children. A 2-dose regime of trivalent inactivated vaccine is required to generate protective levels of hemagglutination inhibiting (HAI) antibodies. A vaccine preparation with enhanced immunogenicity is therefore desir...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-251
更新日期:2011-05-21 00:00:00
abstract:BACKGROUND:Sheeppox (SPP) and goatpox (GTP) caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively of the genus Capripoxvirus in the family Poxviridae, are severely afflicting small ruminants' production systems in Africa and Asia. In endemic areas, SPP and GTP are controlled using vaccination with live...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-0969-8
更新日期:2018-04-02 00:00:00
abstract::Six major Hepatitis C virus (HCV) genotypes and hundreds of subtypes have been identified globally. All these genotypes are generally studied for epidemiology, their vaccine development and clinical management. This article comments the frequency distribution of various HCV genotypes circulate in different areas/distr...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-203
更新日期:2010-08-26 00:00:00
abstract:BACKGROUND:Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it. METHOD:In this study, we attempted to eluci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0654-8
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE:The aim of the present study was to investigate the association between maternal seropositivity of rubella, He...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0813-6
更新日期:2017-07-26 00:00:00
abstract::During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-le...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-131
更新日期:2009-08-24 00:00:00
abstract::From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool con...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-174
更新日期:2010-07-28 00:00:00
abstract:BACKGROUND:Human papillomavirus (HPV) bivalent and quadrivalent vaccines have been widely implemented in worldwide organized immunization programs. A nonavalent HPV vaccine is now available in several countries. The objective was to describe the fraction of squamous non-invasive high-grade cervical intraepithelial lesi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0879-1
更新日期:2017-11-06 00:00:00
abstract:BACKGROUND:The Hepatitis B Virus X protein (HBx) plays a major role in hepatocellular carcinoma (HCC) development, however, its contribution to tumor invasion and metastasis has not been established so far. Heat shock protein 90 alpha (HSP90alpha) isoform is an ATP-dependent molecular chaperone that maintains the activ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-45
更新日期:2010-02-20 00:00:00
abstract::Duck hepatitis virus (DHV-1) is a worldwide distributed picornavirus that causes acute and fatal disease in young ducklings. Recently, the complete genome of DHV-1 has been determined and comparative sequence analysis has shown that possesses the typical picornavirus organization but exhibits several unique features. ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-147
更新日期:2011-03-31 00:00:00
abstract:BACKGROUND:The global spread of the highly pathogenic avian influenza H5N1 virus has stimulated interest in a better understanding of the mechanisms of H5N1 dispersal, including the potential role of migratory birds as carriers. Although wild birds have been found dead during H5N1 outbreaks, evidence suggests that othe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-71
更新日期:2008-06-04 00:00:00
abstract:BACKGROUND:The threat of recurring influenza pandemics caused by new viral strains and the occurrence of escape mutants necessitate the search for potent therapeutic targets. The dependence of viruses on cellular factors provides a weak-spot in the viral multiplication strategy and a means to interfere with viral multi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-108
更新日期:2010-05-26 00:00:00
abstract:BACKGROUND:The effect of antiviral therapy in chronic hepatitis B (CHB) on reducing the risk of long-term complications (LTCs) remains unclear so far. To study whether long-term nucleos(t)ide analogues therapy can reduce the risk of long-term complications. METHODS:We searched MEDLINE, EMBASE, OVID, the Cochrane Centr...
journal_title:Virology journal
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1743-422X-8-72
更新日期:2011-02-15 00:00:00
abstract:AIM:To evaluate the efficacy and safety of Entecavir (ETV) plus adefovir (ADV) for chronic hepatitis B (CHB) patients after multiple nucleos(t)ide analogue (NAs) failure treatment. METHODS:Hepatitis B e antigen (HBeAg)-positive patients who had a suboptimal response or developed resistance to two or more previous NAs ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-162
更新日期:2013-05-25 00:00:00
abstract:BACKGROUND:In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0353-x
更新日期:2015-08-08 00:00:00
abstract:BACKGROUND:Using a murine model of parainfluenza virus infection (mPIV1 or Sendai virus; SeV), we compared the inflammatory responses to lethal and sub-lethal infections in inbred DBA/2 mice. METHODS:Mice were intranasally inoculated with either 1.6×10(3) or 1.6×10(5) infectious units (IU) of SeV or diluent control. C...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-357
更新日期:2013-12-21 00:00:00
abstract::Earlier, we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than empty/intermediate capsids. Some of the packaged viral RNAs appear t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-16
更新日期:2009-02-05 00:00:00
abstract:BACKGROUND:Human papillomaviruses (HPVs), a group of non-enveloped small viruses with double-stranded circular DNA which lead to multiple skin diseases such as benign warts, are commonly seen in clinics. The current HPV detection systems aim mainly at mucosal HPVs, however, an efficient clinical approach for cutaneous ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01373-3
更新日期:2020-07-09 00:00:00
abstract::Retroviral transduction involves integrase-dependent linkage of viral and host DNA that leaves an intermediate that requires post-integration repair (PIR). We and others proposed that PIR hijacks the host cell double-strand DNA break (DSB) repair pathways. Nevertheless, the geometry of retroviral DNA integration diffe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-11
更新日期:2008-01-22 00:00:00
abstract:BACKGROUND:Enzootic nasal tumor virus (ENTV) is a betaretrovirus of sheep (ENTV-1) and goats (ENTV-2) associated with neoplastic transformation of epithelial cells of the ethmoid turbinate. Confirmation of the role of ENTV in the pathogenesis of enzootic nasal adenocarcinoma (ENA) has yet to be resolved due to the inab...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0795-4
更新日期:2017-07-26 00:00:00
abstract:BACKGROUND:Herpesviruses are ubiquitous pathogens that infect and cause recurrent disease in multiple animal species. Feline herpesvirus-1 (FHV-1), a member of the alphaherpesvirus family, causes respiratory illness and conjunctivitis, and approximately 80% of domestic cats are latently infected. Oral administration of...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-34
更新日期:2014-02-22 00:00:00
abstract:BACKGROUND:Tembusu virus is a newly emerging flavivirus that caused egg-drop syndrome in ducks in China. TMUV envelope protein is a major structural protein locates at the surface of tembusu virus particle. During tembusu virus infection, envelope protein plays a pivotal role in induction of neutralizing antibody. Howe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1052-1
更新日期:2018-09-17 00:00:00
abstract:BACKGROUND:Rabbit haemorrhagic disease virus (RHDV), as the pathogeny of Rabbit haemorrhagic disease, can cause a highly infectious and often fatal disease only affecting wild and domestic rabbits. Recent researches revealed that it, as one number of the Caliciviridae, has some specialties in its genome, its reproducti...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-494
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. C...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-45
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice. METHODS:A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and imm...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0597-0
更新日期:2016-11-14 00:00:00
abstract:BACKGROUND:On 19 February 2013, the first patient infected with a novel influenza A H7N9 virus from an avian source showed symptoms of sickness. More than 349 laboratory-confirmed cases and 109 deaths have been reported in mainland China since then. Laboratory-confirmed, human-to-human H7N9 virus transmission has not b...
journal_title:Virology journal
pub_type: 历史文章,杂志文章
doi:10.1186/1743-422X-11-185
更新日期:2014-11-03 00:00:00
abstract:BACKGROUND:Ebola viruses (EBOVs) cause severe hemorrhagic fever with a high mortality rate. At present, there are no licensed vaccines or efficient therapies to combat EBOV infection. Previous studies have shown that both humoral and cellular immune responses are crucial for controlling Ebola infection. CD8+ T cells pl...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-111
更新日期:2012-06-13 00:00:00
abstract:BACKGROUND:The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent primarily in reno-urinary tract, reactivating only in case of a compromised immune system. The seroepi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0752-2
更新日期:2017-04-24 00:00:00