Abstract:
BACKGROUND:Previous studies on bladder cancer have shown nodal involvement to be an independent indicator of prognosis and survival. This study aimed at developing an objective method for detection of nodal metastasis from molecular profiles of primary urothelial carcinoma tissues. METHODS:The study included primary bladder tumor tissues from 60 patients across different stages and 5 control tissues of normal urothelium. The entire cohort was divided into training and validation sets comprised of node positive and node negative subjects. Quantitative expression profiling was performed for a panel of 70 genes using standardized competitive RT-PCR and the expression values of the training set samples were run through an iterative machine learning process called genetic programming that employed an N-fold cross validation technique to generate classifier rules of limited complexity. These were then used in a voting algorithm to classify the validation set samples into those associated with or without nodal metastasis. RESULTS:The generated classifier rules using 70 genes demonstrated 81% accuracy on the validation set when compared to the pathological nodal status. The rules showed a strong predilection for ICAM1, MAP2K6 and KDR resulting in gene expression motifs that cumulatively suggested a pattern ICAM1 > MAP2K6 > KDR for node positive cases. Additionally, the motifs showed CDK8 to be lower relative to ICAM1, and ANXA5 to be relatively high by itself in node positive tumors. Rules generated using only ICAM1, MAP2K6 and KDR were comparably robust, with a single representative rule producing an accuracy of 90% when used by itself on the validation set, suggesting a crucial role for these genes in nodal metastasis. CONCLUSION:Our study demonstrates the use of standardized quantitative gene expression values from primary bladder tumor tissues as inputs in a genetic programming system to generate classifier rules for determining the nodal status. Our method also suggests the involvement of ICAM1, MAP2K6, KDR, CDK8 and ANXA5 in unique mathematical combinations in the progression towards nodal positivity. Further studies are needed to identify more class-specific signatures and confirm the role of these genes in the evolution of nodal metastasis in bladder cancer.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Mitra AP,Almal AA,George B,Fry DW,Lenehan PF,Pagliarulo V,Cote RJ,Datar RH,Worzel WPdoi
10.1186/1471-2407-6-159subject
Has Abstractpub_date
2006-06-16 00:00:00pages
159issn
1471-2407pii
1471-2407-6-159journal_volume
6pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:The associations between microRNAs and lung cancer have received increasing attention. This study assess the association between polymorphisms in miR-135a-2, miR-219-2 and miR-211 genes and the risk of lung cancer, as well as the gene-environment interaction between these polymorphisms and cooking oil fume e...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2784-1
更新日期:2016-09-23 00:00:00
abstract:BACKGROUND:Gonadotropin-releasing hormone (GnRH) and its receptor (GnRHR) are both expressed by a number of malignant tumors, including those of the breast. In the latter, both behave as potent inhibitors of invasion. Nevertheless, the signaling pathways whereby the activated GnRH/GnRHR system exerts this effect have n...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-550
更新日期:2012-11-23 00:00:00
abstract:BACKGROUND:There are three main therapeutic strategies, namely expectant management (dilation and curettage only), prophylactic chemotherapy and prophylactic total hysterectomy for treating older women with complete hydatidiform mole (CHM). However, the scientific community has so far, not unanimously accepted the abov...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3749-8
更新日期:2017-11-09 00:00:00
abstract:BACKGROUND:Uterine leiomyosarcoma (ULMS) is an aggressive form of soft tissue tumors. The molecular heterogeneity and pathogenesis of ULMS are not well understood. METHODS:Expression profiling data were used to determine the possibility and optimal number of ULMS molecular subtypes. Next, clinicopathological character...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3568-y
更新日期:2017-09-11 00:00:00
abstract:BACKGROUND:To the best of our knowledge, no study has reported mediastinal shift accompanied with obstructive atelectasis due to bulky primary esophageal tumor components treated with adaptive radiotherapy and concurrent chemotherapy. CASE PRESENTATION:Here we report the case of a 65-year-old male patient diagnosed wi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6505-4
更新日期:2020-01-06 00:00:00
abstract:BACKGROUND:The efficacy of immune checkpoint blockade in the treatment of microsatellite instability (MSI)-high tumors was recently reported. Therefore, the acquisition of histological specimens is desired in cases of unresectable solid pancreatic lesions (UR SPLs). This study investigated the efficacy of endoscopic ul...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07588-5
更新日期:2020-11-11 00:00:00
abstract:BACKGROUND:Therapeutic regulation of PPARdelta activity using selective agonists has been proposed for various disorders. However, the consequences of altered peroxisome proliferator-activated receptor delta (PPARdelta) activity in the context of intestinal tumourigenesis remain somewhat unclear. Contradictory evidence...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-6-113
更新日期:2006-05-03 00:00:00
abstract:BACKGROUND:Patients undergoing surgery for esophageal cancer have a high risk for postoperative deterioration of lung function and pulmonary complications. This is partly due to one-lung ventilation during thoracotomy. This often accounts for prolonged stay on intensive care units, delayed postoperative reconvalescence...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-017-3400-8
更新日期:2017-06-14 00:00:00
abstract:BACKGROUND:Cancer stem cells (CSCs) are thought to play important roles in therapy-resistance. In this study, we induced cancer stem-like cells from hepatocellular carcinoma (HCC) cell lines using a unique medium, and examined their potential for resistance to anti-cancer drugs. METHODS:The human HCC cell lines SK-HEP...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-722
更新日期:2014-09-27 00:00:00
abstract:BACKGROUND:Women with BRCA1 or BRCA2 mutations have a substantially increased risk of breast and ovarian cancer compared with the general population. Therefore, prophylactic mastectomy (PM) and bilateral salpingo-oophorectomy (BSO) have been proposed as risk-reduction strategies for BRCA1/2 mutation carriers. We aimed ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-101
更新日期:2008-04-14 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate epigenetic status of cyclin A1 in human papillomavirus-associated cervical cancer. Y. Tokumaru et al., Cancer Res 64, 5982-7 (Sep 1, 2004)demonstrated in head and neck squamous-cell cancer an inverse correlation between cyclin A1 promoter hypermethylation and TP53 mutati...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-6-55
更新日期:2006-03-08 00:00:00
abstract:BACKGROUND:The objective of this study is to investigate the role and experience of early stage non-small cell lung cancer (NSCLC) patient in decision making process concerning treatment selection in the current clinical practice. METHODS:Stage I-II NSCLC patients (surgery 55 patients, SBRT 29 patients, median age 68)...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-3986-5
更新日期:2018-01-15 00:00:00
abstract:BACKGROUND:Alterations in the redox balance are involved in the origin, promotion and progression of cancer. Inter-individual differences in the oxidative stress regulation can explain a part of the variability in cancer susceptibility.The aim of this study was to evaluate if polymorphisms in genes codifying for the di...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-861
更新日期:2014-11-21 00:00:00
abstract:BACKGROUND:Merkel cell carcinoma (MCC) is a rare cutaneous neoplasm with increasing incidence, aggressive behavior and poor prognosis. Somatostatin receptors (SSTR) are expressed in MCC and represent a potential target for both imaging and treatment. METHODS:To non-invasively assess SSTR expression in MCC using PET an...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-268
更新日期:2014-04-17 00:00:00
abstract:BACKGROUND:Diabetes is related with increased cancer mortality across multiple cancer types. Its role in lung cancer mortality is still unclear. We aim to determine the prognostic value of fasting plasma glucose (FPG) and diabetes mellitus in patients with locally advanced non-small cell lung cancer (NSCLC) treated wit...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5370-5
更新日期:2019-02-21 00:00:00
abstract:BACKGROUND:Phase 3 studies suggest that induction chemotherapy (ICT) of cisplatin and 5-fluorouracil plus docetaxel (TPF) is effective but toxic for patients with squamous-cell carcinoma of the head and neck (SCCHN). Dose-dense chemotherapy may yield favorable outcomes compared with standard-dose chemotherapy, yet the ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07347-6
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:It has been suggested that the B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) gene plays an oncogenic role in several types of human cancer, but the status of Bmi-1 amplification and expression in ovarian cancer and its clinical/prognostic significance are unclear. METHODS:The methods of im...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-133
更新日期:2010-04-08 00:00:00
abstract:BACKGROUND:Malignant glioma is a common primary tumor of the central nervous system. Brevican, an abundant extracellular matrix component in the adult brain, plays a critical role in the process of glioma. The mechanisms for the highly invasive behavior of gliomas are still poorly understood. The aim of this study was ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-607
更新日期:2012-12-19 00:00:00
abstract:BACKGROUND:Myeloid sarcoma (MS), also known as chloroma, is an extramedullary manifestation of malignant primitive myeloid cells. Previously, only small studies investigated clinical and imaging features of MS. The purpose of this study was to elucidate clinical and imaging features of MS based upon a multicenter patie...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/s12885-019-6357-y
更新日期:2019-11-27 00:00:00
abstract:BACKGROUND:The Nijmegen Breakage Syndrome is a chromosomal instability disorder characterized by microcephaly, growth retardation, immunodeficiency, and increased frequency of cancers. Familial studies on relatives of these patients indicated that they also appear to be at increased risk of cancer. METHODS:In a candid...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-9-181
更新日期:2009-06-12 00:00:00
abstract:BACKGROUND:Methylentetrahydrofolate reductase (MTHFR) plays a major role in folate metabolism and consequently could be an important factor for the efficacy of a treatment with 5-fluorouracil. Our aim was to evaluate the prognostic and predictive value of two well characterized constitutional MTHFR gene polymorphisms f...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-14-58
更新日期:2014-02-03 00:00:00
abstract:BACKGROUND:A universal hallmark of cancer cells is the change in their glycosylation phenotype. One of the most frequent alterations in the normal glycosylation pattern observed during carcinogenesis is the enhancement of α(1,6)linked fucose residues of glycoproteins, due to the up-regulation of the α(1,6)fucosyltransf...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-508
更新日期:2011-12-07 00:00:00
abstract:: ...
journal_title:BMC cancer
pub_type: 社论,历史文章
doi:10.1186/s12885-018-5020-3
更新日期:2018-11-12 00:00:00
abstract:BACKGROUND:Following the recommendation of lung cancer screening in the US, screening committees in several European countries are reviewing the evidence for implementing national programmes. However, inadequate participation from high-risk groups poses a potential barrier to its effectiveness. The present study examin...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4430-6
更新日期:2018-05-02 00:00:00
abstract:BACKGROUND:Lymphotoxin-alpha (LTA) is a pro-inflammatory cytokine with anti-tumor activity. The objective of this study was to determine whether LTA polymorphisms influence the presence of cancer. METHODS:LTA polymorphisms C804A (rs1041981, T60N) and T495C (rs2229094, C13R) were determined in 1,536 consecutive autopsy...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-235
更新日期:2008-08-13 00:00:00
abstract:BACKGROUND:It is well known that osteopontin (OPN) plays an important role in tumor progression and that a high OPN expression level in several tumor entities correlates with poor prognosis in cancer patients. However, little is known about the prognostic relevance of the OPN mRNA splice variants. METHODS:We analyzed ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-131
更新日期:2012-04-02 00:00:00
abstract:BACKGROUND:To obtain high-yield histological samples by targeted prostate cancer (PCa) biopsy is the current trend compared with transrectal ultrasound (TRUS)-guided systematic histological biopsy, which is regarded as the gold standard for prostate cancer (PCa) diagnosis. In this paper, we present a targeted PCa imagi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06801-9
更新日期:2020-05-14 00:00:00
abstract:BACKGROUND:Glioblastoma multiforme (GBM) is the most aggressive of the primary brain tumors, with a grim prognosis despite intensive treatment. In the past decades, progress in research has not significantly increased overall survival rate. METHODS:The in vitro antineoplastic effect and mechanism of action of Casiopei...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-156
更新日期:2012-04-27 00:00:00
abstract:BACKGROUND:Lifestyle factors, including food and nutrition, physical activity, body composition and reproductive factors, and single nucleotide polymorphisms (SNPs) are associated with breast cancer risk, but few studies of these factors have been performed in the Japanese population. Thus, the goals of this study were...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-13-565
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Estrogen receptor positive breast cancers have high recurrence rates despite tamoxifen therapy. Breast cancer stem/progenitor cells (BCSCs) initiate tumors, but expression of estrogen (ER) or progesterone receptors (PR) and response to tamoxifen is unknown. Interleukin-6 (IL-6) and interleukin-8 (IL-8) may i...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-733
更新日期:2014-09-30 00:00:00